Anti flag ab205606

The following specific antibodies were used in this study: Anti‐γH2AX (#2577), anti‐γ‐H2AX (#9718) and anti‐GAPDH (#51332), anti‐H4K8ac (#2594), anti‐ATM (#2873), anti‐ATM (#92356), anti‐pATM (#5883S), anti‐ATR (#13934), anti‐pATR (#2853), anti‐CHK1(#2360), anti‐pCHK1 (#2348), anti‐CHK2 (#6334), anti‐pCHK2 (#2197), anti‐FLAG (8146), anti‐MYC (71D10) and anti‐TIP60 (#12058) were all purchased from Cell Signaling Technology (Boston, MA). anti‐TIP60 (#10398) was purchased from Abnova. anti‐ATM (#ET‐1606‐20) was purchased from Human Technology. Anti‐FAM135B (SAB2104963) was purchased from Sigma‐Aldrich (Merck Darmstadt, Germany). Anti‐FLAG (ab205606) and anti‐53BP1 (ab36823) antibodies were acquired from Abcam (England); anti‐GST (HT601) was acquired from Santa Cruz (Dallas, USA); bleomycin (BLM, radiomimetic drug), etoposide (ETO) and cisplatin (CDDP, a chemotherapy drug that can cause DNA double‐chain rupture as a DNA damage inducer) purchased from Selleck (America). We describe all the antibodies and drugs in Tables S1 and S2.

Reagents included: cell Signaling Senescence β-Galactosidase Staining Kit (CST, Boston, USA); fetal bovine serum, DMEM/F12 medium, RPMI medium1640, and DMEM, penicillin and PBS (GE™ Hyclone, Utah, USA); Doxycycline (Sigma, St. Louis, Missouri, USA); Cell Counting Kit-8 (Dojindo Molecular Technologies, Kumamoto, Japan); Lipofectamine 3000 (Invitrogen, California, USA); dual-specific luciferase assay kit (Promega, Wisconsin, USA); fibroblast growth factor (FGF) and epidermal growth factor (EGF) (PeproTech, New Jersey, USA). Antibodies including anti-ZNF24 (ab254636), anti-GAPDH (ab76523), anti-CyclinD1 (ab16663), anti-CyclinB1 (ab32053), anti-CyclinE1 (ab33911), anti-cMYC (ab71676), anti-C-Jun (ab15475), anti-fra1 (ab252421), anti-HA (ab236632), anti-flag (ab205606), anti-β-catenin (ab32503), anti-β-actin (ab8227), anti-TCF1 (ab188865), anti-LEF1 (ab2324), anti-Cleaved Caspase-9 (ab2324), and anti-Cleaved Caspase-3 (ab2302) were purchased from Abcam Co., Ltd, Cambridge, UK.