Photothrombotic stroke was induced in all rats, as described previously [4 (link)]. Briefly, the rats were fixed in a stereotactic frame under deep anesthesia (isoflurane 2.5%). A template with an aperture encompassing the right sensorimotor cortex (5 mm anterior to 5 mm posterior and 0.5 mm to 5.5 mm lateral to the bregma) was put on the exposed skull. A cold light source (Olympus KL1500LCD, Mainz, Germany) was positioned over the aperture. Rose Bengal (Sigma-Aldrich, Darmstadt, Germany) in NaCl 0.9% was intravenously injected and thereafter the determined cerebral region was illuminated for 15 min. During the whole procedure, body temperature was maintained at 37 ± 0.5 °C by a feedback-controlled heating system.
Kl1500lcd
Manufactured by Olympus
Sourced in Germany
The KL1500LCD is a compact and versatile LED light source designed for use with microscopes. It provides adjustable illumination intensity and color temperature to optimize visual clarity and contrast. The KL1500LCD features an LCD display for easy control and monitoring of the light output.
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5 protocols using kl1500lcd
1
Photothrombotic Stroke Induction in Rats
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Photothrombotic Stroke Induction in Rats
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All animals were subjected to photothrombotic stroke as described elsewhere in detail [9 (link)]. Under deep anesthesia (isoflurane 2.5% (cp-pharma, Burgdorf, Germany), rats were placed in a stereotactic frame. A template with an aperture covering the right sensorimotor cortex was put on the exposed skull (5 mm anterior to 5 mm posterior and 0.5 mm to 5.5 mm lateral to the bregma). Rose Bengal (Sigma, Darmstadt, Germany) in NaCl 0.9% was administered intravenously and then the sensorimotor cortex was illuminated with a cold light source (Olympus KL1500LCD, Main, Germany) for 15 min.
3
Photothrombotic Stroke Induction in Rats
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A photothrombotic lesion was inflicted within the right cerebral cortex of all animals as described recently [44 (link)]. Briefly, after induction of anesthesia (isoflurane 2.5%), rats were fixed in a stereotactic frame and the skull was exposed. Aluminum foil with an aperture was put on the head using the coordinates [45 ] encompassing the right sensorimotor cortex (5 mm anterior to 5 mm posterior and 0.5 to 5.5 mm lateral to the Bregma). Rose Bengal (Sigma-Aldrich, St. Louis, MO, USA) dissolved in 0.9% NaCl was injected intravenously. Thereafter, the sensorimotor cortex was illuminated for 15 min with a cold light source (Olympus KL1500LCD, Mainz, Germany) which was positioned over the aluminum foil. Body temperature was maintained at 37 ± 0.5 °C by a feedback-controlled heating system during the whole surgery.
4
Geometric Morphometric Analysis of Tsetse Fly Wings
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Forty-one individual G. p. palpalis flies collected at the border region in Cameroon had wings in a state suitable for morphometric analysis. Wings were secured on a microscope slide using a drop of 1:100 diluted Faure’sche solution (33.3 mL distilled water, 13.3% v/v glycerol, 20 g gum arabicum, 33.3% v/v chloralhydrate) on the wing root. They were covered with a cover glass and dried overnight. Pictures were taken using a Olympus SZX16 binocular (Olympus, Hamburg, Germany) with upward light (Olympus KL1500 LCD, Olympus, Hamburg, Germany) employing 10× magnification and scale bar added using Cell^F program (Olympus, Hamburg, Germany). Nine landmarks were selected for analysis using the CLIC package [36 ] as described previously [37 (link)]. COO was used to digitalize the landmarks. Procrustes superimposition (centring of configuration of landmarks, scaling, rotation) and comparison of centroid sizes was done using MOG. Samples were segregated according to their genetic group or sex.
5
Pathogenicity Assay for Solanum lycopersicum
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Pathogenicity assays were conducted using Solanum lycopersicum (‘Moneymaker’, Kiepenkerl Bruno Nebelung). Surface sterilization of the seeds was performed as previously described [59 (link),60 (link)]. Ten-day-old seedlings were infected via root dipping in 50 mL of a 1 × 107 spores per ml suspension, grown for 21 days under long-day conditions and evaluated according to the protocol described previously [60 (link),68 (link)]. Briefly, plant height, weight, and longest leaf length were measured and compared with the means of water-inoculated (mock) plants, each set as 100%. Each parameter was characterized as either healthy (≥80%; (1)) or as a mild (60–79%; (2)), strong (40–59%; (3)) or very strong symptom (≤39%; (4)). The mean disease level of the three parameters determined the disease score of the individual plant. Plants with a mean disease level of 1–1.99, 2–2.99, 3–3.99 or 4 were rated as healthy, as having weak symptoms, as having strong symptoms, or as having very strong symptoms, respectively. Stacking diagrams show the relative number of plants assigned to the respective symptom category. Hypocotyl discoloration was observed by binocular microscopy (SZX12-ILLB2-200, illuminated with KL1500 LCD, Olympus).
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