Hrp affinipure goat anti mouse igg

Manufactured by Jackson ImmunoResearch

HRP-AffiniPure goat anti-mouse IgG is a secondary antibody conjugate used for the detection of mouse immunoglobulin G (IgG) in various immunoassay techniques. The antibody is affinity-purified and conjugated to horseradish peroxidase (HRP), enabling it to be used as a detection reagent in applications such as ELISA, Western blotting, and immunohistochemistry.

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Lab products found in correlation

4 protocols using hrp affinipure goat anti mouse igg

Immunofluorescence and Western Blot Analysis

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Mouse anti-SAS6, rabbit anti-cortactin, and mouse anti-γ-tubulin: Santa Cruz; rabbit anti-STIL and mouse anti-TRIO: Abcam; rabbit anti-GEF-H1 and rabbit anti-integrin β1 (total form), mouse anti-GAPDH, rabbit anti-GOLPH2, rabbit anti-β-actin, rabbit anti-paxillin, mouse anti-γ-tubulin, and rabbit anti-NMIIA: GeneTex; mouse anti-α-tubulin and mouse anti-acetylated tubulin: Sigma-Aldrich; rat anti-α-tubulin: ABD; mouse anti-paxillin: BD; mouse anti-Rac1 and mouse anti-integrin β1 (active form): Millipore; rabbit anti-TRIO, Alexa Fluor 488 phalloidin, Alexa Fluor 488-anti-rat IgG, Alexa Fluor 488-anti-rabbit IgG, Alexa Fluor 488-anti-mouse IgG, Alexa Fluor 568-anti-mouse IgG, Alexa Fluor 568-anti-rabbit IgG, Alexa Fluor 680-anti-rabbit IgG, and DAPI: Thermo Fisher; and HRP-AffiniPure mouse anti-rabbit IgG and HRP-AffiniPure goat anti-mouse IgG: Jackson ImmunoResearch.

Cheng H.W., Hsiao C.T., Chen Y.Q., Huang C.M., Chan S.I., Chiou A, & Kuo J.C. (2019). Centrosome guides spatial activation of Rac to control cell polarization and directed cell migration. Life Science Alliance, 2(1), e201800135.

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Antibodies and Dilutions for Immunofluorescence and Western Blotting

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The sources of antibodies and the dilutions used in experiments were as follows: rabbit anti-β-PIX (Millipore 07-1450, Darmstadt, Germany; dilution for Western blotting: 1/1000; dilution for immunofluorescence: 1/500); mouse anti-paxillin (BD Bioscience 610052, San Jose, California, USA; dilution for immunofluorescence: 1/1000); mouse anti-GAPDH (GeneTex GTX627408, Hsinchu, Taiwan; dilution for Western blotting: 1/1000); DNase I (Sigma-Aldrich SAB2702031, Saint Louis, Missouri, USA; dilution for immunofluorescence: 1:1000); Alexa Fuor 488-antimouse IgG (Jackson ImmunoResearch 715-546-151/J1, West Grove, Philadelphia, USA; dilution for immunofluorescence: 1/300); Alexa Fuor 568-antimouse IgG (Invitrogen A11031; dilution for immunofluorescence: 1/300); Alexa Fuor 568-anti-rabbit IgG (Invitrogen A11036; dilution for immunofluorescence: 1/300); Alexa Fuor 647-antimouse IgG (Jackson ImmunoResearch 715-606-151/J1; dilution for immunofluorescence: 1/300). Alexa Fluor 488 phalloidin (A12379; dilution for immunofluorescence: 1/400) and Alexa Fluor 568 phalloidin (A12380; dilution for immunofluorescence: 1/400) were obtained from Invitrogen. HRP-AffiniPure goat antimouse IgG (115-035-174; dilution for Western blotting: 1/5000) and HRP-AffiniPure goat anti-rabbit IgG (211-032-171; dilution for Western blotting: 1/5000) were obtained from Jackson ImmunoResearch.

Yu H.W., Chen Y.Q., Huang C.M., Liu C.Y., Chiou A., Wang Y.K., Tang M.J, & Kuo J.C. (2015). β-PIX controls intracellular viscoelasticity to regulate lung cancer cell migration. Journal of Cellular and Molecular Medicine, 19(5), 934-947.

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Integrin-Mediated Cellular Adhesion Analysis

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Mouse anti-paxillin (BD 610052); rat anti-active integrin β1 (9EG7; BD 553715); rabbit-anti-Tom20 (Santa Cruz sc-11415); mouse-anti-MYH1/2 (Santa Cruz sc-53088); rabbit anti-fibronectin (Santa Cruz sc-9068); rabbit anti-GAPDH (GeneTex GTX100118); rabbit anti-β-actin (GeneTex GTX100313); rabbit anti-integrin β1 (GeneTex GTX128839); rabbit anti-paxillin (GeneTex GTX125891); rabbit anti-pY397-FAK (GeneTex GTX129840); rabbit anti-kinesin-1 (Abcam AB167429); rabbit anti-GFP (Abcam AB290); mouse anti-α-tubulin (Sigma T5168); mouse anti-MYH2 (Thermo Fisher 14-6503-80); rabbit anti-FAK (Thermo Fisher AHO0502); Alexa Fluor 488 phalloidin (Thermo Fisher A12379); Alexa Fluor 568 phalloidin (Thermo Fisher A12380); Alexa Fluor 488-anti-rabbit IgG (Thermo Fisher A11034); Alexa Fluor 488-anti-mouse IgG (Thermo Fisher A11029); Alexa Fluor 488-anti-rat IgG (Thermo Fisher A11006); Alexa Fluor 568-anti-rabbit IgG (Thermo Fisher A11036); Alexa Fluor 568-anti-mouse IgG (Thermo Fisher A11031); DAPI (Thermo Fisher D1306); Mitotracker Red (Thermo Fisher M7512); HRP-AffiniPure mouse anti-rabbit IgG (Jackson ImmunoResearch 211-032-171); and HRP-AffiniPure goat anti-mouse IgG (Jackson ImmunoResearch 115-035-174).

Lin J.W., Huang Y.M., Chen Y.Q., Chuang T.Y., Lan T.Y., Liu Y.W., Pan H.W., You L.R., Wang Y.K., Lin K.H., Chiou A, & Kuo J.C. (2021). Dexamethasone accelerates muscle regeneration by modulating kinesin-1-mediated focal adhesion signals. Cell Death Discovery, 7, 35.

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Characterization of Focal Adhesion Proteins

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The sources of the antibodies and their dilutions were as follows: mouse anti-paxillin (BD Bioscience 610052; dilution for immunofluorescence: 1/1000); rabbit anti-fibronectin (Santa Cruz Sc-9068; dilution for Western blotting: 1/500); rabbit anti-FAK (invitrogen AHO0502; dilution for Western blotting: 1/100); rabbit anti-FAK-pY397 (GeneTex GTX129840; dilution for Western blotting: 1/500); rabbit anti-integrin · 1 (CD29) (GeneTex GTX128839; dilution for Western blotting: 1/1000); mouse anti-integrin β1 (B44) (Millipore MAB2259Z; dilution for Western blotting: 1/100); mouse anti-α-tubulin (Sigma T1568; dilution for Western blotting: 1/4000); Alexa Fluor 488 phalloidin (Invitrogen A12379; dilution for immunofluorescence: 1/300); Alexa Fluor 568-anti-mouse IgG (Invitrogen A11031; dilution for immunofluorescence: 1/300); HRP-AffiniPure goat anti-mouse IgG (Jackson ImmunoResearch 115-035-174; dilution for immunofluorescence: 1/50000); and HRP-AffiniPure mouse anti-rabbit IgG (Jackson ImmunoResearch 211-032-171; dilution for immunofluorescence: 1/50000).

Hsiao C.T., Cheng H.W., Huang C.M., Li H.R., Ou M.H., Huang J.R., Khoo K.H., Yu H.W., Chen Y.Q., Wang Y.K., Chiou A, & Kuo J.C. (2017). Fibronectin in cell adhesion and migration via N-glycosylation. Oncotarget, 8(41), 70653-70668.

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