Crp latex hs

Venepuncture blood samples were taken, with the subjects in the supine position using vacuum tubes. Serum was obtained after clotting for 30 to 60 minutes at room temperature and centrifuging for 10 minutes at 2000g. All samples were stored at -80°C. The serum samples were analysed on a Hitachi 911 multianalyser, Roche, Mannheim, FRG. Apolipoprotein A-1 (APOA-1) and Apolipoprotein B (APO B) were analysed in serum using an immunoturbidimetry method and high sensitivity CRP (hs-CRP) was analysed using a latex enhanced immunoturbidimetry method CRP (Latex) HS, both from Roche/Boehringer Mannheim, FRG. LDL and HDL cholesterol were measured by direct homogeneous assays based on detergent treatment of the serum, N-geneous^™ HDL-c and N-geneous^™ LDL reagents, respectively, from Genzyme Corporation (Cambridge, MA, USA). Venous samples were drawn with a minimum of stasis in siliconized evacuated Stabilyte^™ tubes (Biopool^®, Umeå, Sweden) for plasma samples. The tPA, PAI-1 and tPA-PAI-1 complex concentrations in Stabilyte plasma [22 (link)] were determined using enzyme-linked immunosorbent assays using reagent kits from Biopool^® (Umeå, Sweden). All coefficients of variation were under 7.5%.

Blood samples were taken after a supine rest of 30 minutes. The inflammation parameters of sTNF-R1 and IL-6 were analyzed by the Department of Medicine III, University Clinics Halle (Saale). After a 10-min centrifugation (20°C, 1,500 rpm, Acc = 9, Dcc = 3), the plasma was collected and stored at −80°C. The cytokines were determined using commercially available sandwich enzyme-linked immunosorbent assays (ELISAs: IL-6, Opteia, BD Biosciences, Heidelberg, Germany; TNF-R1, Boehringer Mannheim, Mannheim, Germany).
The determination of CRP was undertaken by the Institute of Laboratory Medicine, Clinical Chemistry and Molecular Diagnostics at the Leipzig University Clinics. The laboratory has been accredited according to the accreditation norms ISO 15180 and ISO 17025. Serum levels of high-sensitivity CRP (hsCRP) were measured using a high-sensitivity immunoturbidimetric method (CRP [Latex] HS, Roche, Mannheim, Germany) on a Hitachi autoanalyzer (Roche Diagnostics, Mannheim, Germany).