Rabbit anti neun antibody

Manufactured by Thermo Fisher Scientific

The Rabbit anti-NeuN antibody is a primary antibody used to detect the NeuN (Neuronal Nuclei) protein, which is a biomarker for mature neurons. This antibody can be used in various immunodetection techniques, such as immunohistochemistry and immunocytochemistry, to identify and quantify neuronal populations.

Automatically generated - may contain errors

Lab products found in correlation

Recombinant egf, by R&D Systems (1 mentions) Rabbit anti ki67 antibody, by Thermo Fisher Scientific (1 mentions) Mouse anti brdu antibody, by Thermo Fisher Scientific (1 mentions) Fibroblast growth factor (fgf), by R&D Systems (1 mentions) Rabbit anti map2, by Merck Group (1 mentions) 710 laser scanning confocal microscope, by Zeiss (1 mentions) Rabbit anti sox2 antibody, by Merck Group (1 mentions)

2 protocols using rabbit anti neun antibody

Immunohistochemical Analysis of Neural Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rabbit anti-Ki67 antibody (Cat #PA1–21520; dilution 1:500 for IF), Mouse anti-BrdU antibody (Cat #MS-1058-P0; dilution 1:500 for IF), Rabbit anti-NeuN antibody (Cat #711054; dilution 1:500 for IF), Rabbit anti-Nestin antibody (Cat # PA5–11887; dilution 1:500 for IF), anti-DCX antibody (Cat # 48–1200; dilution 1:500 for IF), and anti-BMP4 antibody (Cat # PA5–27288; dilution 1:500 for IF) were purchased from ThermoFisher. Rabbit anti-MAP2 (Cat # AB5622; dilution 1:500 for IF) was obtained from EMD Millipore. Mouse anti-PPARα antibody (Cat # sc-398394; dilution 1:500 for IF) was bought from Santa Cruz Biotechnology. BrdU, chemically known as 5-Bromo-2′-deoxyuridine (Cat #B5002–1G) was purchased from Sigma. Recombinant EGF (Cat # 236-EG) and FGF (Cat #233-FB) were purchased from R&D system.

Roy A., Kundu M., Chakrabarti S., Patel D.R, & Pahan K. (2021). Oleamide, a sleep-inducing supplement, upregulates doublecortin in hippocampal progenitor cells via PPARα. Journal of Alzheimer's disease : JAD, 84(4), 1747-1762.

+ Open protocol

+ Expand

Neural Progenitor and Neuron Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

One brain section of ACN-Ctr and ACN-KO mice at P7 or at 1 mo of age was selected for the analysis of tdTomato reporter expression in neural progenitor cells and mature neurons, respectively. The following primary antibodies were used: rabbit anti-SOX2 antibody (1:800; Millipore), rabbit anti-NeuN antibody (1:200, Thermo Fisher). Immunolabeling was performed as previously described (Meneghini et al., 2010) ; then, sections were scanned with a Zeiss 710 confocal laser scanning microscope. Sox2 and NeuN positive cells were analyzed in their entire z-axis to assess the co-expression with the tdTomato reporter.

Tapella L., Soda T., Mapelli L., Bortolotto V., Bondi H., Ruffinatti F.A., Dematteis G., Stevano A., Dionisi M., Ummarino S., Di Ruscio A., Distasi C., Grilli M., Genazzani A.A., D'Angelo E., Moccia F, & Lim D. (2020). Deletion of calcineurin from GFAP-expressing astrocytes impairs excitability of cerebellar and hippocampal neurons through astroglial Na⁺ /K⁺ ATPase. Glia, 68(3).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!