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Tsc2 4308

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TSC2 #4308 is a primary antibody that recognizes the TSC2 protein. TSC2 is a tumor suppressor protein that is a component of the TSC1/TSC2 complex, which functions as a negative regulator of the mTOR signaling pathway. This antibody can be used for applications such as western blotting and immunoprecipitation to detect and study the TSC2 protein.

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Lab products found in correlation

Bca quantification, by Thermo Fisher Scientific (1 mentions) S6 2317, by Cell Signaling Technology (1 mentions) Total 4e bp1 9644, by Cell Signaling Technology (1 mentions) Criterion xt, by Bio-Rad (1 mentions) Cleaved caspase 3 9664, by Cell Signaling Technology (1 mentions) β actin 4970, by Cell Signaling Technology (1 mentions) P53 2524, by Cell Signaling Technology (1 mentions) P ampkαt172, by Cell Signaling Technology (1 mentions) P p38 t180 y182, by Cell Signaling Technology (1 mentions) Pakt t308, by Cell Signaling Technology (1 mentions) P tsc2 s939, by Cell Signaling Technology (1 mentions) P tsc2 t1462, by Cell Signaling Technology (1 mentions) Phospho 4ebp1 t37 46, by Cell Signaling Technology (1 mentions) Tbc1d7, by Cell Signaling Technology (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Pakt s473, by Cell Signaling Technology (1 mentions) Abl 93, by Developmental Studies Hybridoma Bank (1 mentions) Gapdh, by Abcam (1 mentions) Vhl sc 5575, by Santa Cruz Biotechnology (1 mentions) Irdye 680, by LI COR (1 mentions)

3 protocols using tsc2 4308

1

Western Blot Analysis of Cell Signaling

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Cells lysates were collected using Laemmli buffer (0.05 M Tris-HCl at pH 6.8, 1% SDS, 10% glycerol, 0.1% β-mercaptoethanol), quantified using BCA quantification (Thermo Fisher Scientific) and resolved using Criterion XT pre-cast gels (BioRad) followed by transfer to PVDF membranes. Antibodies used are as follows: pS6S240/244 #5264, S6 #2317, p53 #2524, p4E-BP1T37/46 #2855, total 4E-BP1 #9644, cleaved caspase-3 #9664, cleaved PARP #9544, pAKTT473 #4060, TSC2 #4308, β-actin #4970; all from Cell Signaling Technology and used at 1:1000 dilutions. All uncropped western blots can be found in Supplementary Fig. 9.
Bowling S., Di Gregorio A., Sancho M., Pozzi S., Aarts M., Signore M., D. Schneider M., Martinez-Barbera J.P., Gil J, & Rodríguez T.A. (2018). P53 and mTOR signalling determine fitness selection through cell competition during early mouse embryonic development. Nature Communications, 9, 1763.
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2

Antibody validation for protein analysis

Cited 3 times
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Antibodies against phospho-S6K(T389) (#9205), S6K (#9202), phospho-4E-BP1(T37/46) (#9459), 4E-BP1 (#9452), p-Akt(T308) (#9275), p-Akt(S473) (#9271), Akt (#9272), p-AMPKα(T172) (#2535), AMPKα (#2532), p-p38(T180/Y182) (#9216), p38 (#9212), p-TSC2(T1462) (#3611), p-TSC2(S939) (#3615), TSC2 (#4308), TSC1 (#4906) and TBC1D7 (#14949) proteins were purchased from Cell Signaling Technology. An antibody against HIF-1α (GTX127309) was purchased from GeneTex. Antibodies detecting mouse LAMP2 (ABL-93) and human LAMP2 (A4B4) were obtained from Developmental Studies Hybridoma Bank. Monoclonal antibodies recognizing human and mouse α-tubulin (#T9026), FLAG-tag sequence (#F1804) and the peroxisomal marker PMP70 (#SAB4200181) were purchased from Sigma. All antibodies were used at 1:1,000 dilution for western blotting, except for the total TSC2, p-4E-BP1, total 4E-BP1 and total p38 antibodies that were used at 1:2,000. For immunofluorescence experiments, the TSC2 (validated in this study and in previous reports4 (link)18 (link)), TSC1 (validated in this study), LAMP2 and PMP70 (validated in ref. 29 (link)) antibodies were used at 1:200 dilution.
Demetriades C., Plescher M, & Teleman A.A. (2016). Lysosomal recruitment of TSC2 is a universal response to cellular stress. Nature Communications, 7, 10662.
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3

Western Blot Analysis of Cell Signaling

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Cells or tissues were lysed in RIPA buffer with protease and phosphatase inhibitors and lysates were processed using standard methods. We used the following primary antibodies: GAPDH (ab9485, 1:2000; Abcam), VHL (sc-5575, 1:250; Santa Cruz), HIF1a (NB100-105, 1:500; Novus Biologicals), TSC1 (ab32936, 1:500; Abcam), TSC2 (4308, 1:1000; Cell Signalling). Secondary antibodies conjugated to IRDye 680 or 800 were used (Li-Cor). Fluorescent signals were quantified using the Odyssey Infrared Imaging System (Li-Cor).
Espana-Agusti J., Tuveson D.A., Adams D.J, & Matakidou A. (2015). A minimally invasive, lentiviral based method for the rapid and sustained genetic manipulation of renal tubules. Scientific Reports, 5, 11061.
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