Human ifn γ

Manufactured by BioLegend

Sourced in United States

Human IFN-γ is a recombinant protein that functions as the cytokine interferon-gamma. It is used in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Human il 2, by BioLegend (2 mentions) Il 17, by BioLegend (1 mentions) Ifn β, by PBL Assay Science (1 mentions) Elisa for mouse ifn γ, by BD (1 mentions) Mouse ifn γ elisa, by BioLegend (1 mentions) Anti flag antibody, by BioLegend (1 mentions) Dynabeads human t activator cd3 cd28 for t cell expansion and activation, by Thermo Fisher Scientific (1 mentions) Zombie uv dye, by BioLegend (1 mentions) Alexa fluor 488 goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555 goat anti rat igg antibody, by BioLegend (1 mentions)

Close spelling variants of this product

ELISA MAX Deluxe Set Human IFN-γ Human ELISA kits for IFN-γ FITC anti-human IFN-γ antibody Anti-human IFN-γ antibody PE-Cy7 anti-human IFN-γ LEGEND MAX™ Human IFN-γ ELISA Kit LEGEND MAX human IFN-γ kit PE/Cyanine7 anti-human IFN-γ antibody PerCP anti-human IFN-γ ELISA MAX Standard Set Human IFN-γ

5 protocols using human ifn γ

Quantification of Secreted Proteins and Antibody Binding

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the concentration of secreted proteins after stimulation, human IFN-γ (#430101; BioLegend) and human IL-2 (#431801; BioLegend) detection kits were used. To quantify anti-CD45 and anti-SLAMF6 antibody binding, polystyrene high binding microplates (Corning) were coated with immobilized CD45 (#14197-H08H; SinoBiological) or SLAMF6 (#11945-H08H; SinoBiological) recombinant ectodomain proteins, respectively. To quantify anti-CD3 and/or anti-SLAMF6 antibody binding to CD3, immobilized SLAMF6 KO Jurkat T cell lysates were used as antigen bait. Primary antibody binding was detected using a secondary HRP goat antibody recognizing human Fc (#SSA001-200; Sino-Biologic).

Gartshteyn Y., Askanase A.D., Song R., Bukhari S., Dragovich M., Adam K, & Mor A. (2022). SLAMF6 compartmentalization enhances T cell functions. Life Science Alliance, 6(2), e202201533.

+ Open protocol

+ Expand

Cytokine Quantification in Cell Cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell culture supernatants were analyzed by ELISA for mouse IFN-γ (BD Bioscience), IL-9 (BioLegend), IL-17 (BioLegend) or IFN-β (PBL Assay Science) or for human IFN-γ (BioLegend) or human IL-9 (BioLegend) according to the manufacturer’s instructions.

Benoit-Lizon I., Jacquin E., Rivera Vargas T., Richard C., Roussey A., Dal Zuffo L., Martin T., Melis A., Vinokurova D., Shahoei S.H., Baeza Garcia A., Pignol C., Giorgiutti S., Carapito R., Boidot R., Végran F., Flavell R.A., Ryffel B., Nelson E.R., Soulas-Sprauel P., Lawrence T, & Apetoh L. (2022). CD4 T cell-intrinsic STING signaling controls the differentiation and effector functions of TH1 and TH9 cells. Journal for Immunotherapy of Cancer, 10(1), e003459.

+ Open protocol

+ Expand

Mature moDC Cytokine and Surface Marker Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mature moDC cell culture supernatants were tested for the levels of IL-12 (mouse IL-12 ELISA cat# 433607 and human IL-12 cat# 431704) and IFNγ (mouse IFNγ ELISA cat# 430804 and human IFNγ cat# 430104) using enzyme-linked immunosorbent assay (ELISA) kits (BioLegend). Mature DCs were stained immediately for flow cytometry. moDC differentiation and maturation was determined via labeling with fluorescence-conjugated antibodies specific for MHC class I (cat# 311403) and class II (cat# 361706), CD80 (cat# 305219), CD252 (cat# 326307), CD40 (cat# 334309), CD86 (cat# 305421), CD83 (cat# 305305), CCR7 (cat# 353203), CCR5 (cat# 313707), CCR6 (cat# 353415), CCR3 (cat# 310707) and CD205 (cat# 342203) (BioLegend). The expression of these cell surface markers was determined by flow cytometry and analyzed using FlowJo software (TreeStar).

Penafuerte C., Feldhammer M., Mills J.R., Vinette V., Pike K.A., Hall A., Migon E., Karsenty G., Pelletier J., Zogopoulos G, & Tremblay M.L. (2017). Downregulation of PTP1B and TC-PTP phosphatases potentiate dendritic cell-based immunotherapy through IL-12/IFNγ signaling. Oncoimmunology, 6(6), e1321185.

+ Open protocol

+ Expand

Multiparameter Analysis of Immune Cells

Cited 4 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

Anti-human PD-L1/CD274, -PE and –APC (Clone# 29E.2A3, catalog#329745, 329706, 329708), anti-FLAG antibody (Clone# L5, catalog#637304), Alexa Fluor 555 goat anti-rat IgG antibody (Catalog#405420), Alexa Fluor 467 goat anti-rat IgG antibody(Catalog#405416), human IFNγ (Catalog#570208), human IFNα (Catalog#592704), human IL-2 (Catalog#589104), and Zombie UV dye (Catalog#77474) were purchased from Biolegend (San Diego, CA, USA). Human IFNβ (Catalog#8499-1F) was purchased from R&D Systems (Minneapolis, MN 55413). Heat Inactivated human AB serum was purchased from Innovative Research, Inc (Catalog# IPLA-SERAB-27146, MI 48377, USA). Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation was purchased from ThermoFisher Scientific (Catalog# 11131D, Waltham, MA, USA). The CF33-hNIS-Δ (CF33-hNIS-ΔF14.5L) virus [24 (link), 25 (link)], CF33-GFP [ref.26 (link)], and the CF33-hNIS-antiPDL1 virus [23 (link)] were generated in our lab as previously described. Anti-sodium/Iodide Symporter (hNIS) monoclonal antibody was purchased from EMD Millipore Corp (Catalog# MAB3564, Billerica, MA, USA). Goat anti-rabbit IgG (H+L) Alexa Fluor 555 antibody (Catalog# A21434) and Goat anti-mouse Alexa Fluor 488 antibody (Catalog# A11029) were purchased from Invitrogen Corporation (Carlsbad, CA, USA).

Zhang Z., Yang A., Chaurasiya S., Park A.K., Lu J., Kim S.I., Warner S.G., Yuan Y.C., Liu Z., Han H., Von Hoff D., Fong Y, & Woo Y. (2021). CF33-hNIS-antiPDL1 Virus Primes Pancreatic Ductal Adenocarcinoma for Enhanced anti-PD-L1 Therapy. Cancer gene therapy, 29(6), 722-733.

+ Open protocol

+ Expand

Quantification of Secreted Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

To determine the concentration of secreted proteins after stimulation, human and mouse IL-2 (Biolegend), human IFN-γ (Biolegend), and mouse Granzyme B (Invitrogen) kits were used according to the manufacturer’s protocols. Cells were stimulated as indicated.

Peled M., Dragovich M.A., Adam K., Strazza M., Tocheva A.S., Vega I.E, & Mor A. (2018). EF Hand domain family member D2 is required for T cell cytotoxicity. Journal of immunology (Baltimore, Md. : 1950), 201(9), 2824-2831.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!