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Snap tag t7 2

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The SNAP-tag-T7-2 is a protein fusion tag that can be used for the detection and purification of recombinant proteins expressed in E. coli. The SNAP-tag is a 20 kDa protein that can be fused to the target protein and then reacted with specific synthetic substrates for various applications.

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SNAP-tag

2 protocols using snap tag t7 2

1

IPTG-Induced Ena and Dia Purification

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Dia or Ena were induced with 0.5 mM isopropylthio-β-galactoside (IPTG) for 16 hr at 16°C and purified from Talon Metal Affinity Resin. Ena was gel purified on S20010/300GL and Dia was dialyzed against formin buffer and stored at −80°C. SNAP tagging used SNAP-tag-T7-2(NEB) with a flexible linker (GGSGGS) between tag and start codon, and labeling was per manufacturer.
Bilancia C.G., Winkelman J.D., Tsygankov D., Nowotarski S.H., Sees J.A., Comber K., Evans I., Lakhani V., Wood W., Elston T.C., Kovar D.R, & Peifer M. (2014). Enabled Negatively Regulates Diaphanous-Driven Actin Dynamics In Vitro and In Vivo. Developmental Cell, 28(4), 394-408.
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2

Purification and SNAP-tagging of Ena and Dia

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Dia or Ena were induced with 0.5mM IPTG 16h at 16°C, and purified from Talon Metal Affinity Resin. Ena was gel purified on S20010/300GL and Dia was dialyzed against formin buffer, and stored at −80°C. SNAP-tagging used SNAP-tag-T7-2(NEB) with a flexible linker (GGSGGS) between tag and start codon, and labeling was per manufacturer.
Bilancia C.G., Winkelman J.D., Tsygankov D., Nowotarski S.H., Sees J.A., Comber K., Evans I., Lakhani V., Wood W., Elston T.C., Kovar D.R, & Peifer M. (2014). Enabled Negatively Regulates Diaphanous-Driven Actin Dynamics In Vitro and In Vivo. Developmental Cell, 28(4), 394-408.
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