Buffer reagents

All materials were used as received, unless otherwise noted. Protein G-conjugated magnetic microbeads were purchased from ThermoFisher Scientific, and buffer reagents were purchased from Sigma. Human anti-EPCAM antibody was purchased from Novus Biologicals (NB100-65094). Anti-lamin B2 and anti-beta actin antibodies were purchased from Abcam (ab8983 and ab8226, respectively).

Glyceryl triacetate, buffer reagents, β-glycerophosphate, erythro-9-(2-hydroxy-3-nonyl) adenine, α, β-methyleneadenosine 5’-diphosphate, and 2-mercaptoethanol were purchased from Sigma (Sigma, St. Louis, MO, USA). A mouse anti-human CD73 antibody and a horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG antibody were obtained from AbD Serotec (AbD Serotec, Raleigh, NC, USA). A HRP-conjugated goat anti-mouse IgG antibody was purchased from Jackson ImmunoResearch (Jackson ImmunoResearch, Westgrove, PA, USA). A mouse anti-adenosine receptor A_2A antibody was obtained from Upstate (Upstate, Billerica, MA, USA). A goat anti-adenosine kinase antibody, mouse anti-α tubulin antibody, HRP-conjugated donkey anti-goat IgG antibody, and HRP-conjugated goat anti-mouse IgM antibody were obtained from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Absolute ethanol was from Pharmco (Pharmco, Brookfield, CT, USA).

Mouse recombinant HGF and human recombinant BMP9 were purchased from R&D Systems (Minneapolis, MN, USA). p38α/β MAPK inhibitor SB203580 was from Calbiochem (La Jolla, CA, USA). TGF-β activated kinase (TAK)1 inhibitor (5Z)-7-oxozeaenol was from Tocris Bioscience (Bristol, UK). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and trypsin-EDTA were from Gibco-Invitrogen (Barcelona, Spain). Penicillin, streptomycin, propidium iodide, DNA oligos, and buffer reagents were from Sigma-Aldrich (Tres Cantos, Madrid, Spain). HEPES and bovine serum albumin (fraction V, fatty-acid free) from Panreac AppliChem (Castellar del Valles, Barcelona, Spain). Nucleospin RNA kit (Macherey-Nagel) from Cultek (Madrid, Spain). SuperScript III RNase H Reverse Transcriptase was from Invitrogen. Oligo-dT was from Roche Diagnostics (Sant Cugat del Valles, Barcelona, Spain). ECL reagent is from Thermo-Fisher Scientific (Madrid, Spain). Caspase-3 substrate was obtained from PharMingen (San Diego, CA, USA). Primary antibodies used in this study are listed in Table S2.

Human IgG was obtained from I.V.-Globulin SN injection product from GC Pharma (Yongin, Korea). Acetic acid glacial was purchased from Duksan (Ansan, Korea). Tergazyme was purchased from Alconox (White Plains, NY, USA). All other reagents including buffer reagents and chemicals, unless indicated otherwise, were obtained from Sigma-Aldrich (St. Louis, MO, USA).

Buffer reagents were from Sigma-Aldrich and gel electrophoresis reagents were from Bio-Rad. Nitrocellulose membranes and enhanced chemiluminescence reagent were from Perkin-Elmer and Pierce, respectively. Anti-Clathrin [X22], anti-VAMP2 and anti-AP180 were from Abcam. Anti-CALM and anti-β-Actin was from Sigma. Anti-GFP was from Roche. All horseradish peroxidase-conjugated secondary antibodies were from DAKO. Dulbecco’s Modified Eagle’s Medium (DMEM), fetal bovine serum (FBS), phosphate buffered saline (PBS), Trypsin-EDTA and Alexa Fluor 594 labelled transferrin were from Invitrogen. Paraformaldehyde was from Scharlau Chemie. All reagents were of analytical grade unless specified otherwise.