Ab193355

Anti‐PlexinB2 antibody used for Western blotting analysis was purchased from Abcam (ab193355; dilution 1:500). EGFR antibody was provided by Enzo Life Sciences (ALX‐804‐064‐C100; dil. 1:500), while anti‐phospho‐EGFR‐specific antibodies (directed to p‐Tyr1068; ab5644; dil. 1:500) were from Abcam. Anti‐vinculin (V4505; dil. 1:1,000) and anti‐VSV‐G (clone P5D4; dil. 1:1,000) were provided by Sigma‐Aldrich. Anti‐phospho‐p44/42‐MAPK (Erk1/2; Thr202/Tyr204; #4370; dil. 1:1,000), anti‐p44/42‐MAPK (Erk1/2; 137F5; #4695; dil. 1:1,000), and anti‐phospho‐S6 ribosomal protein (Ser240/244; #2215; dil. 1:1,000) antibodies were from Cell Signaling.

Antibodies used for western blot analysis (WB) or immunofluorescence (IF) were as follows:
anti-β-actin, Sigma A1978 (host species: mouse), dilution 1:10,000 for WB
anti-EGFR, Cell Signaling Technology 4267 (rabbit), 1:1000 for WB
anti-human nuclear antigen (HNA), Millipore MAB1281 (mouse), 1:250 for IF
anti-integrin β1 (human-specific clone TS2/16), Santa Cruz sc-53711 (mouse), 1:300 for IF
anti-laminin, Millipore MAB1905 (rat), 1:200 for IF
anti-MBP, CST 78896 (rabbit), 1:100 for IF
anti-MET, Cell Signaling Technology 8198 (rabbit), 1:1000 for WB
anti-pMLC2 (Ser19), Cell Signaling Technology 3671 (rabbit), 1:100 for IF
anti-PDGFRα, Cell Signaling Technology 3174 (rabbit), 1:1000 for WB
anti-PECAM-1/CD31, BD Biosciences 553370 (rat), 1:300 for IF
anti-Plexin-B1, R&D systems AF3749 (goat), 1:400 for WB
anti-Plexin-B2, extracellular domain, R&D systems AF5329 (sheep), 1:400 for WB, 1:100 for IF
anti-Plexin-B2, intracellular domain, Abcam ab193355 (rabbit), 1:1000 for WB
anti-Plexin-B3, R&D systems AF4958 (sheep), 1:400 for WB
anti-Sema4B, Proteintech 16934-1-AP (rabbit), 1:400 for WB
anti-Sema4C, LSBio C150056 (sheep), 1:400 for WB
anti-YAP/TAZ, Santa Cruz sc101199 (mouse), 1:100 for IF.
For isotype control IF staining, we used serum-purified IgG fractions from the same host species as the primary antibody at similar concentrations (Chromepure IgG, Jackson ImmunoResearch).