The effect of different concentrations of copper on Caco-2 proliferation was determined using real-time cellular analysis (RTCA). After 24 h stimulation, cells (8 × 103 cells/well) were seeded in several E-Plate 16 dishes (ACEA Biosciences Inc., CA, United States) for proliferation assays. The plates were kept in the cell incubator at 37°C with 5% CO2 for 3–4 days. The cell index and growth curves were automatically recorded on the xCELLigence RTCA System (ACEA Biosciences Inc., CA, United States).
E plate 16 dishes
Manufactured by Agilent Technologies
Sourced in United States
The E-Plate 16 is a multi-well cell culture plate designed for real-time monitoring of cellular processes. It features 16 individual wells that allow for simultaneous analysis of multiple experimental conditions.
Automatically generated - may contain errors
4 protocols using e plate 16 dishes
1
Copper's Effects on Caco-2 Proliferation
2
LINC00673 Effects on Cell Proliferation
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The effect of LINC00673 on cell proliferation was determined using real-time cellular analysis (RTCA). Twenty-four hours after transfection, cells (5×103 cells/well) were seeded in several E-Plate 16 dishes (ACEA Biosciences Inc, CA, USA) for proliferation assays. The plates were kept in the cell incubator at 37 °C with 5% CO2 for 4–6 days. The cell index and growth curves were automatically recorded on the xCELLigence RTCA System (ACEA Biosciences Inc, CA, USA).
3
Real-Time Cell Proliferation Assay
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Cell proliferation was analyzed using RTCA [19 (link)]. First, 50 μl of cell culture medium was added to E-Plate 16 dishes (ACEA Biosciences Inc, CA, USA), and the background impedance was measured and displayed as the cell index. Then, cells were seeded in these dishes at a density of 5,000 cells per well, and 200 μl of complete medium was added. After 24 h, cells were treated with 60 μM Res. The cell index, representing the change in impedance and the proliferation of cells, was then obtained.
4
Cell Proliferation, Colony Formation, and Apoptosis Assays
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Cell proliferation was determined using real-time cellular analysis (RTCA). Twenty-four hours after transfection, cells (6 × 103 cells/well) were seeded in several E-Plate 16 dishes (ACEA Biosciences Inc, CA, USA) for proliferation assays. The plates were kept in the cell incubator at 37°C with 5% CO2 for 6 days. The cell index and growth curves were automatically recorded on the xCELLigence RTCA System (ACEA Biosciences Inc, CA, USA). For colony formation assay, cells transfected with siRNAs were plated in 6-well plates at 700 cells/well then cultured for 2 weeks. The cells were fixed and stained for 30 min in 35% methanol solution with 1% crystal violet; then, a number of foci >100 cells were counted. For apoptosis analyses, Annexin V/PI staining of cells was carried out. At 48 hours after transfection, cells were harvested, washed, and stained with FITC Annexin V Apoptosis Detection kit (Beyotime, China). And then, apoptosis cells were determined by a flow cytometer (Guava easyCyte HT, Millipore). All assays were performed in triplicate.
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