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Crystallography screens

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Crystallography screens are laboratory equipment used for the preparation and screening of protein crystal samples. They provide a controlled environment for the crystallization process, which is a crucial step in determining the three-dimensional structure of proteins and other biomolecules.

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Lab products found in correlation

Protease inhibitor tablet, by Merck Group (5 mentions) Dithiothreitol (dtt), by GoldBio (5 mentions) Restriction enzymes, by Integrated DNA Technologies (3 mentions) Ampicillin amp, by GoldBio (2 mentions) Ampicillin, by GoldBio (1 mentions) 13c6 d glucose, by Cambridge Isotopes (1 mentions)

5 protocols using crystallography screens

1

Purification and Characterization of Phosphatase

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Dithiothreitol (DTT) and ampicillin (AMP) were purchased from GoldBio. Restriction enzymes and primers were purchased from Integrated DNA Technologies. Protease-inhibitor tablets were purchased from Sigma-Aldrich. All other buffers and reagents were purchased from Sigma-Aldrich or Fisher. The substrate p-nitrophenyl phosphate (pNPP) was synthesized using published methods.24 (link) Crystallography screens, trays, and coverslips were purchased from Hampton Research.
Shen R., Crean R.M., Olsen K.J., Corbella M., Calixto A.R., Richan T., Brandão T.A., Berry R.D., Tolman A., Loria J.P., Johnson S.J., Kamerlin S.C, & Hengge A.C. (2022). Insights into the importance of WPD-loop sequence for activity and structure in protein tyrosine phosphatases. Chemical Science, 13(45), 13524-13540.
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2

Enzyme Substrate Synthesis Protocol

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Dithiothreitol (DTT) and
ampicillin (AMP)
were purchased from GoldBio. Restriction enzymes were purchased from
Integrated DNA Technologies. Protease-inhibitor tablets were purchased
from Sigma-Aldrich. All other buffers and reagents were purchased
from Sigma-Aldrich or Fisher. The substrates p-nitrophenyl
phosphate (pNPP)27 (link) and p-nitrophenyl phosphorothioate (pNPPS)28 (link) were synthesized using published methods. Crystallography
screens, trays, and coverslips were purchased from Hampton Research.
Shen R., Crean R.M., Johnson S.J., Kamerlin S.C, & Hengge A.C. (2021). Single Residue on the WPD-Loop Affects the pH Dependency of Catalysis in Protein Tyrosine Phosphatases. JACS Au, 1(5), 646-659.
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3

SsoPTP Expression and Purification

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Dithiothreitol (DTT) and ampicillin (AMP) were purchased from GoldBio. Restriction enzymes were purchased from Integrated DNA Technologies. Protease-inhibitor tablets were purchased from Sigma-Aldrich. All other buffers and reagents were purchased from Sigma-Aldrich or Fisher. The substrate p-nitrophenyl phosphate (pNPP) was synthesized using published methods.18 (link) Crystallography screens, trays, and coverslips were purchased from Hampton Research. For labeled protein expression, deuterium oxide (D2O), 15N-ammonium chloride, and 13C6-D-glucose were purchased from Cambridge Isotope Laboratories (Tewksbury, MA).
An E. coli codon-optimized SsoPTP gene was obtained from General Biosystems. The gene was cloned into a pET-45b (+) vector using NcoI and SacI restriction sites. These restriction sites do not include the histidine tag and allow for tagless SsoPTP purification.
Pinkston J., Jo J., Olsen K.J., Comer D., Loria J.P., Johnson S.J, & Hengge A.C. (2021). Significant Loop Motions in the SsoPTP Protein Tyrosine Phosphatase Allow for Dual General Acid Functionality. Biochemistry, 60(38), 2888-2901.
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4

Synthesis and Characterization of Novel Substrates

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Dithiothreitol (DTT) and ampicillin (AMP) were purchased from GoldBio. Restriction enzymes were purchased from Integrated DNA Technologies. Protease-inhibitor tablets were purchased from Sigma-Aldrich. All other buffers and reagents were purchased from Sigma-Aldrich or Fisher. The substrates p-nitrophenyl phosphate (pNPP) 26 and p-nitrophenyl phosphorothioate (pNPPS) 27 were synthesized using published methods. Crystallography screens, trays, and coverslips were purchased from Hampton Research.
Shen R., Crean R., Johnson S., Kamerlin S.C.L., & Hengge A.C. (2021). A Single Residue on the WPD-Loop Affects the pH Dependency of Catalysis in Protein Tyrosine Phosphatases.
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5

Synthesis and Characterization of Phosphate Substrates

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Dithiothreitol (DTT) and ampicillin (AMP) were purchased from GoldBio. Restriction enzymes were purchased from Integrated DNA Technologies. Protease-inhibitor tablets were purchased from Sigma-Aldrich. All other buffers and reagents were purchased from Sigma-Aldrich or Fisher. The substrates p-nitrophenyl phosphate (pNPP) 27 and p-nitrophenyl phosphorothioate (pNPPS) 28 were synthesized using published methods. Crystallography screens, trays, and coverslips were purchased from Hampton Research.
Shen R., Crean R., Johnson S., Kamerlin S.C.L., & Hengge A.C. (2021). A Single Residue on the WPD-Loop Affects the pH Dependency of Catalysis in Protein Tyrosine Phosphatases.
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