Fei teneo lv scanning electron microscope

For SEM analysis lenses were isolated from the eyes of normal mice and mice with EAU and the vitreous gently removed from the lenses prior to fixation in sodium cacodylate buffer containing 2.5% of glutaraldehyde and 1% of paraformaldehyde. Three control and 6 uveitic lenses were used per time point analyzed. The samples were rinsed and then postfixed in 1% osmium tetroxide. Serial dehydration in ethyl alcohol was followed by critical point drying (Autosamdri‐931, Tousimis, Rockville, MD, USA). A thin layer of gold was sputtered onto the surface of the samples (Cressington 208HR, Ted Pella Inc, Redding, CA, USA) before imaging them in the FEI Teneo LV Scanning Electron Microscope (Thermo Fisher, Hillsboro, OR, USA). Imaging conditions were 2.0 KV with a working distance of 8.7 mm using the Everhart Thornley detector.

Lenses were fixed in 2.5% of glutaraldehyde and 1% of paraformaldehyde in sodium cacodylate buffer. After rinsing, the samples were postfixed in 1% of osmium tetroxide. A serial dehydration in ethyl alcohol was followed by critical point drying (Autosamdri‐931, Tousimis, Rockville, MD). A thin layer of gold was sputtered onto the surface of the samples (Cressington 208HR, Ted Pella Inc, Redding, CA) before imaging in the FEI Teneo LV Scanning Electron Microscope (Thermo Fisher, Hillsboro, OR). Imaging conditions were 2.0 KV with a working distance of 8.7 mm using the Everhart Thornley detector.