Mouse blood was withdrawn from the retro-orbital sinus using a Microvette EDTA-coated capillary blood collection tubes (Fisher Scientific, Pittsburgh, PA). The blood (120 μl) was immediately centrifuged at 1500 × g for 15 min at 4 °C. Fifty microliter of plasma was recovered and stored at −80 °C for analysis of oxidized amino acids. Mouse aortas from wild-type and GLA null mice transfections were stored in antioxidant solution buffer A at −80 °C. Well-minced mouse aortas were homogenized with a Tissue Tearor (Biospec Products, Bartlesville, OK) and lysed in 1% Triton X-100 lysate buffer. The clarified aorta lysate was analyzed with the help of immunoblotting after BCA assay.
Microvette edta coated capillary blood collection tubes
Manufactured by Thermo Fisher Scientific
Microvette EDTA-coated capillary blood collection tubes are designed for the collection of capillary blood samples. The tubes are coated with EDTA, an anticoagulant, to prevent blood clotting during the collection process.
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Lab products found in correlation
2 protocols using microvette edta coated capillary blood collection tubes
1
Oxidized Amino Acids in Mouse Aorta
2
Plasma Oxidation and Aorta Analysis
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