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Foxo3

Manufactured by Enzo Life Sciences
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FoxO3 is a protein that functions as a transcription factor. It plays a role in the regulation of cellular processes such as cell cycle, apoptosis, and metabolism.

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Lab products found in correlation

Enhanced chemiluminescent substrate, by Merck Group (2 mentions) Fast mhc, by Abcam (2 mentions) Memcode reversible protein stain kit, by Thermo Fisher Scientific (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) Chemidoc xr, by Bio-Rad (2 mentions) Image lab, by Bio-Rad (2 mentions)

2 protocols using foxo3

1

Western Blot Analysis of Myotube Proteins

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Myotube proteins were collected in radio-immunoprecipitation assay (RIPA) buffer containing a protease inhibitor cocktail (Roche Complete). Equal amounts of total protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes. Equal loading and transfer were confirmed by MemCode Reversible Protein Stain Kit (Thermo Scientific). Membranes were incubated overnight with primary antibodies from Cell Signaling (FoxO3, #2497), Enzo Life Sciences (19S Rpt5/S6a, #PW8770; 19S Rpt6, #PW9265; 20S β1 subunit, #PW8140; 20S β5 subunit, #PW8895), Developmental Studies Hybridoma Bank (Embryonic MHC, #F1.652; type I MHC, #BA-F8), or Abcam (Fast MHC, #ab7784). Secondary antibodies, conjugated to horseradish peroxidase, were detected using an enhanced chemiluminescent substrate (Millipore). Band intensities were captured using a Bio-Rad Chemi Doc XRS and analyzed using Image Lab (BioRad) or ImageJ64 (NIH) software.
Bollinger L.M., Powell J.J., Houmard J.A., Witczak C.A, & Brault J.J. (2015). Skeletal muscle myotubes of the severely obese exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux. Obesity (Silver Spring, Md.), 23(6), 1185-1193.
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2

Western Blot Analysis of Myotube Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Myotube proteins were collected in radio-immunoprecipitation assay (RIPA) buffer containing a protease inhibitor cocktail (Roche Complete). Equal amounts of total protein were separated by SDS-PAGE and transferred to polyvinylidene difluoride (PVDF) membranes. Equal loading and transfer were confirmed by MemCode Reversible Protein Stain Kit (Thermo Scientific). Membranes were incubated overnight with primary antibodies from Cell Signaling (FoxO3, #2497), Enzo Life Sciences (19S Rpt5/S6a, #PW8770; 19S Rpt6, #PW9265; 20S β1 subunit, #PW8140; 20S β5 subunit, #PW8895), Developmental Studies Hybridoma Bank (Embryonic MHC, #F1.652; type I MHC, #BA-F8), or Abcam (Fast MHC, #ab7784). Secondary antibodies, conjugated to horseradish peroxidase, were detected using an enhanced chemiluminescent substrate (Millipore). Band intensities were captured using a Bio-Rad Chemi Doc XRS and analyzed using Image Lab (BioRad) or ImageJ64 (NIH) software.
Bollinger L.M., Powell J.J., Houmard J.A., Witczak C.A, & Brault J.J. (2015). Skeletal muscle myotubes of the severely obese exhibit altered ubiquitin-proteasome and autophagic/lysosomal proteolytic flux. Obesity (Silver Spring, Md.), 23(6), 1185-1193.
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