Log In Sign up
The largest database of trusted experimental protocols

Pathogen universal protocol

Manufactured by Roche
Visit Supplier
Sourced in Switzerland

The Pathogen Universal protocol is a laboratory equipment product designed for the detection and identification of a wide range of pathogens. It provides a standardized approach to sample preparation and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Magna pure 96, by Roche (1 mentions) Magna pure 96 dna and viral na large volume kit, by Roche (1 mentions) Miseq desktop sequencer, by Illumina (1 mentions)

2 protocols using pathogen universal protocol

1

Vaginal Sample Processing for Molecular Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
In the HMC laboratory, the vaginal samples were frozen within 24 h and afterwards transported to the external laboratory for molecular analysis. DNA was extracted from 200 μl samples and eluted in a final volume of 100 μl with the MagNA Pure 96 instrument using the MagNA Pure 96 DNA and Pathogen Universal small Volume Kit and the Pathogen Universal protocol (Roche Diagnostics, Basel, Switzerland). Appropriate positive and negative control samples were included in testing, which were evaluated in the BV qPCR.
van den Tweel M.M., van den Munckhof E.H., van der Zanden M., Molijn A., van Lith J.M, & Boers K.E. (2024). The Vaginal Microbiome Changes During Various Fertility Treatments. Reproductive Sciences, 31(6), 1593-1600.
+ Open protocol
+ Expand
2

Microbial 16S rRNA Gene Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Nucleic acids were extracted from 500 μL STGG medium and eluted in a final volume of 100 μL with the MagNA Pure 96 instrument using the MagNA Pure 96 DNA and Viral NA Large Volume kit and the Pathogen Universal protocol (Roche Diagnostics, Basel, Switzerland). Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene was performed as described elsewhere [20 (link)]. Briefly, a fragment of ~ 464 bp of the V3-V4 region of the 16S rRNA gene was amplified and sequenced with the MiSeq desktop sequencer (Illumina, San Diego, USA).
van den Munckhof E.H., Hafkamp H.C., de Kluijver J., Kuijper E.J., de Koning M.N., Quint W.G, & Knetsch C.W. (2020). Nasal microbiota dominated by Moraxella spp. is associated with respiratory health in the elderly population: a case control study. Respiratory Research, 21, 181.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!