A total volume of 500 mL of Colloidal gold nanoparticles with a mean diameter of 20 nm were produced by reduction of 5 mL of 1.0% Chloroauric acid (Sangon, A602523, China) with 0.1 g of 1.0% sodium citrate according to a previously described method [35 (link)]. Briefly, beakers and flasks were washed with ultrapure water for six times and dried before use. The ultrapure water (500 mL) was heated to boiling point, and then 5 mL of 1.0% Chloroauric acid solution were added quickly into the water under constantly stirring. A total 0.1 g of 1.0% sodium citrate was then added to the water. When the color of the solution changed from blue to bright red, the solution was heated for another 5 mins, and the cooled colloidal gold solution was stored at 4 °C.
Chloroauric acid
Manufactured by Sangon
Sourced in China
Chloroauric acid is a chemical compound with the formula HAuCl4. It is a yellow crystalline solid that is commonly used in laboratory applications as a precursor for the synthesis of gold nanoparticles and other gold-containing materials.
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3 protocols using chloroauric acid
1
Synthesis of 20 nm Colloidal Gold Nanoparticles
2
Gold Nanoparticle Synthesis Protocol
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Chloroauric acid was purchased from Sangon Biotech Co., Ltd. (Shanghai, China). Cysteamine and sodium borohydride were provided by Macklin Biochemical Co., Ltd. (Shanghai, China). Tetramethyl-benzidine (TMB) solution was obtained from Sigma-Aldrich (St. Louis, MO). Other chemical reagents were of analytical reagent grade. All solutions were prepared using ultrapure water with a resistivity of 18.2 MΩ cm.
3
Synthesis of Gold Nanoparticles
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Chloroauric acid was provided by Sangon Biotech (Shanghai) Co., Ltd. Trisodium citrate was obtained from Sigma-Aldrich Co. LLC. Luria-Bertani (L-B) broth power was purchased from Guangdong Huankai Microbial Sci. & Tech. Co., Ltd. All solutions were prepared using ultrapure water from a Milli-Q water purification system.
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