Operetta cls confocal microscope

Manufactured by PerkinElmer

The Operetta CLS confocal microscope is a high-content imaging system designed for a wide range of applications in life science research. The system combines high-resolution confocal imaging with automated image acquisition and analysis capabilities, allowing researchers to capture detailed images and perform quantitative analysis of cellular and subcellular structures.

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Lab products found in correlation

Prism 6, by GraphPad (1 mentions) Columbus image analysis system, by PerkinElmer (1 mentions) Alexa fluor 546 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Ab236284, by Abcam (1 mentions) Hoechst 33342, by Thermo Fisher Scientific (1 mentions) 6 well plate, by Corning (1 mentions)

2 protocols using operetta cls confocal microscope

High-throughput Imaging of Intracellular Parasites

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For the L. donovani and T. cruzi bioassays, images of 4 fields/well, which correspond to a 45% coverage of the well, were acquired with the Operetta CLS confocal microscope (Perkin Elmer) at 20× magnification. The images were analysed with the Columbus^TM software (Perkin Elmer) and the number of mammalian cells and intracellular parasites (recognized by the DRAQ5^TM nuclei staining) were quantified to estimate EC₅₀ and CC₅₀ values (expressed as mean ± one SD), respectively, from DRC plots fitted to a dose-response (variable slope) equation. GraphPad Prism6 (GraphPad Software, San Diego, CA) was used for the analysis of quantitative data.

Benítez D., Franco J., Sardi F., Leyva A., Durán R., Choi G., Yang G., Kim T., Kim N., Heo J., Kim K., Lee H., Choi I., Radu C., Shum D., No J.H, & Comini M.A. (2022). Drug-like molecules with anti-trypanothione synthetase activity identified by high throughput screening. Journal of Enzyme Inhibition and Medicinal Chemistry, 37(1), 912-929.

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Immunostaining of iPSC-derived Endothelial Cells

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iPSCs were cultured in 6-well plates (Corning) and were differentiated into endothelial cells as described earlier. At day 3 cells were fixed in 4% paraformaldehyde (PFA) at room temperature for 20 min, permeabilized and immunostained following standard protocol using anti-Factor VIII antibody (Abcam, ab236284, 1:200) and fluorescently labeled secondary antibody (Invitrogen, Alexa fluor 546 goat anti-rabbit, 1:500). Additionally, nuclei were labeled with Hoechst 33342 (Invitrogen, H3570, 1:5000). Images of cells were automatically captured using an automated Operetta CLS confocal microscope (PerkinElmer) at 20× magnification. Subsequent image capturing was performed using the PerkinElmer Columbus Image Analysis System as previously described^{63 (link)}.

Lansing F., Mukhametzyanova L., Rojo-Romanos T., Iwasawa K., Kimura M., Paszkowski-Rogacz M., Karpinski J., Grass T., Sonntag J., Schneider P.M., Günes C., Hoersten J., Schmitt L.T., Rodriguez-Muela N., Knöfler R., Takebe T, & Buchholz F. (2022). Correction of a Factor VIII genomic inversion with designer-recombinases. Nature Communications, 13, 422.

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