Msd r plex

Urine biomarkers for this study were selected by an expert panel of CKD Biomarkers Consortium members in the preinvestigation stage based on prior work. Urine was collected at baseline and centrifuged, and the supernatant was aliquoted with unique barcodes.¹⁶ (link) Aliquots were kept in continuous laboratory storage at −80 °C until biomarker measurements were made. Personnel conducting biomarker measurements were blinded to clinical outcomes. Urine KIM-1, MCP-1, YKL-40, and EGF were measured on the Luminex platform with a laboratory-developed multiplex assay (Luminex Corporation, Austin, TX). UMOD was measured on the MSD R-PLEX (Meso Scale Diagnostics, LLC, Rockville, MD). Urine α1m was measured on a Siemens BNII nephelometer (Siemens, Inc., Munich, Germany). All measurements except α1m were made in duplicate, and mean values were used in the analyses. If intra-assay coefficient of variation exceeded 15%, the assay was repeated. All UMOD assays were performed with a single lot, which is notable because lot-to-lot variation of UMOD measurements on the MSD R-PLEX can be up to 50%.

Urine KIM-1, MCP-1, YKL-40, and EGF were measured on the Luminex platform with a laboratory-developed multiplex assay (Luminex Corporation, Austin, TX). UMOD was measured on the MSD R-PLEX (Meso Scale Diagnostics, LLC., Rockville, MD). Urine α1m was measured on a Siemens BNII nephelometer (Siemens, Inc., Munich, Germany). Urine aliquots were kept in continuous laboratory storage at −80°C until they were thawed for biomarker measurements. Personnel conducting biomarker measurements were blinded to clinical outcomes. All measurements, except α1m, were made in duplicate, and mean values were used in analyses. If the intra-assay coefficient of variation exceeded 15%, the assay was repeated.