Dm4000 b led microscope system

The tumor specimens were fixed in formalin, embedded in paraffin, cut into 4 μm sections, and stained with hematoxylin and eosin (Yuanye Biotechnology, Shanghai, China). A DM4000 B LED microscope system (Leica Microsystems, Germany) and a DFC 420C 5 M digital microscope camera (Leica Microsystems) were used to examine the slides and take pictures.

Kidney tissues were fixed in 10% neutral buffered formalin and embedded in paraffin. Four-micron sections were cut for haematoxylin and eosin (HE, Yuanye Biotechnology, Shanghai, China) and Masson’s trichrome (Yuanye Biotechnology) staining according to the manufacturer’s protocol. Slides were examined and pictures were taken using a DM4000B LED Microscope System (Leica Microsystems, Germany) and a DFC 420C 5 M Digital Microscope Camera (Leica Microsystems). Tubulointerstitial damage and the degree of interstitial collagen deposition were graded as described previously [25 (link)].

Briefly, 4% paraformaldehyde was added to Stattic-treated PANC-1 and BxPc-3 cells for 30 min for fixation, and the cells were permeabilized with 0.1% Triton X-100 (Solarbio, Dalian, China) for 10 min, and then the cells were blocked with ready-to-use normal goat serum (BOSTER, Wuhan, China) to eliminate nonspecific fluorescence. After washing away goat serum, the cells were hybridized with primary antibodies (Table S1) at 4 °C for 24 h, and then with a fluorescent secondary antibody (DyLight 488-green or 594-red, ProteinTech, Wuhan, China). Finally, the cells were stained with DAPI (Solarbio, Dalian, China), and images were captured using a DM4000 B LED microscope system (Leica Microsystems, Wetzlar, Germany).