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Icr mice

Manufactured by Charles River Laboratories
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Sourced in China, Japan, United States, France

ICR mice are a widely used mouse model in biomedical research. They are outbred, multipurpose mice that exhibit genetic variability, making them suitable for a variety of research applications.

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162 protocols using icr mice

1

Pharmacokinetics of Compound 15 in Mice

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Animals: ICR mice (male, 4–6 weeks old) were purchased from Beijing Vital River Laboratory Animal Technology (Beijing, China). Animals ate food and water freely and were given a daily schedule of dark–light cycle. Before tests, they were housed for at least 12 hours.
The pharmacokinetic analysis of 15 was conducted in male ICR mice which were purchased from Beijing Vital River Laboratory Animal Technology Co. Ltd. The animals were administered a single dose of 2 mg/kg 15 by i.v. after fasting overnight. Blood was collected and centrifuged immediately to isolate plasma. The plasma concentrations were determined using high performance liquid chromatography with HPLC analysis on a Shimadzu Prominence-i LC-2030C 3D system. Pharmacokinetic parameters were estimated by non-compartmental model using WinNonlin 8.2.
Xi J.J., Cao Y., He R.Y., Zhang J.K., Zhao Y.M., Tong Q., Bao J.F., Dong Y.C., Zhuang R.X., Huang J.S., Chen Y, & Liu S.R. (2023). Design, Synthesis and Biological Evaluation of Glycosylated Derivatives of Silibinin as Potential Anti-Tumor Agents. Drug Design, Development and Therapy, 17, 2063-2076.
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2

Ethical ICR Mouse Husbandry Protocol

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ICR mice (30∼34 g, 6∼7 weeks) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) with experimental animal use license SYXK (Lu) 2018 0008. All mice were housed in the specific pathogen free (SPF) feeding system under 12 h/12 h light and dark cycle under a standardized temperature at 20–26 °C and relative humidity at 40%–70% with 5 mice per cage, and were free access to water. All experimental animals were performed in strict compliance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. Procedures were approved by the Ethics Committee for Experimental Animals at State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine (approved on July 3rd, 2020; No. NH-IACUC-2020–047) for minimizing animal suffering. Mice in each cage are distinguished by different colored and marked labels.
Tang Y., Sun L., Zhao Y., Yao J., Feng Z., Liu Z., Zhang G, & Sun C. (2023). UHPLC-ESI-QE-Orbitrap-MS based metabolomics reveals the antioxidant mechanism of icaritin on mice with cerebral ischemic reperfusion. PeerJ, 11, e14483.
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3

Bufalin's Anti-Tumor Effects in Xenograft Mice

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Four-week-old male ICR mice were purchased from the VITAL RIVER Laboratories (Beijing, China). All ICR mice were maintained under pathogen-free and 12 h light/dark cycle conditions, and were free to get food and water. 200 μL of H22 suspension cells (3 × 106) was injected into the flanks of mice to generate xenografts. After subcutaneous inoculation of H22 cells for 3 days, tumors reached approximately 200 mm3 in size. The mice were randomly divided into 4 groups (6 mice in each group): a model group, a positive control group, and bufalin low- and high-dose groups. The mice were treated with bufalin (4 mg/kg), cyclophosphamide (25 mg/kg) and 0.9% saline solution respectively by intraperitoneal injection daily for 14 days. The body weights were recorded every day. The two perpendicular diameters of the tumors were measured and tumor volume was calculated by using the formula V = length (mm) × width (mm)2/2. After 14 days of treatment, mice were sacrificed via cervical dislocation. Then, tumors and organs (heart, liver, spleen, lung, and kidney) were weighted and fixed with 4% paraformaldehyde for further studies. All daily animal care and the experimental procedures were approved by Institutional Animal Care and Use Committee of Peking University (License No. LA2022505). All animal studies have followed the ARRIVE guidelines.
Liu T.T., Yang H., Zhuo F.F., Yang Z., Zhao M.M., Guo Q., Liu Y., Liu D., Zeng K.W, & Tu P.F. (2022). Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy. eBioMedicine, 86, 104353.
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4

EV71 Infection in Neonatal Mice

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The specific-pathogen-free ICR mice were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China), and maintained in a pathogen-free environment (23 ± 2 °C and 55% ± 5% humidity). All mice experiments were approved by the institutional animal care and use committee of the Ocean University of China (OUC-YY-202,201,001). One breeding ICR mouse could give birth to 12–13 pups, which were kept in a single group. Three-day-old neonatal ICR mice (n = 10–12 per group) were intraperitoneally challenged with EV71 at a dose of 105 TCID50 per mice as reported (Zhang et al., 2017b ) followed by an intramuscular injection of PC (5 or 10 mg/kg), Guanidine Hydrochloride (10 mg/kg) or PBS alone. Drugs were administered 12 h, 24 h and 48 h post-infection. The mice were monitored daily for 7 days to assess survival rate, clinical score and body weight. Signs of disease were evaluated by using a graded clinical score (0, health; 1, lethargy; 2, hind limb weakness; 3, single limb paralysis; 4, double limb paralysis; 5, dying or death).
Sun J., Ma X., Sun L., Zhang Y., Hao C, & Wang W. (2023). Inhibitory effects and mechanisms of proanthocyanidins against enterovirus 71 infection. Virus Research, 329, 199098.
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5

Behavioral Evaluation of ICR Mice

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Adult male Institute of Cancer Research (ICR) mice, weighing 18–20 g, were provided by the Vital River Laboratories (Qualified No. SYXK 2016-0011, Beijing, China). All animals were housed in plastic cages in a controlled environment at the temperature of 23 ± 2 °C and 60 ± 5% humidity with an alternating 12 h light, 12 h dark cycle, with access to water and diet ad libitum. All behavioral evaluations were performed during the day (8:00 a.m. to 8:00 p.m.). The animal experiments were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals and performed under the approval and supervision of the Animal Ethics Committee of the Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences (Registration Number: #IMPLAD2017030715, Approval Date: 7 March 2017).
Wang S., Wang C., Yu Z., Wu C., Peng D., Liu X., Liu Y., Yang Y., Guo P, & Wei J. (2018). Agarwood Essential Oil Ameliorates Restrain Stress-Induced Anxiety and Depression by Inhibiting HPA Axis Hyperactivity. International Journal of Molecular Sciences, 19(11), 3468.
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6

Neonatal ICR Mouse Protocol

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Institute of Cancer Research (ICR) mice (within 24 h after birth) were purchased from Vital River Laboratory Animal Technology Co., Ltd. in Beijing, China (certificate number, SCXK [Jing] 2016–0006). All of the experiments involving animals were carried out in accordance with the National Institutes of Health Guide for Care and Use of Laboratory Animals [45 ], and the Institutional Animal Care and Use Committee of Beijing University of Chinese Medicine approved the protocol (BUCM-4-2018050701-2065, 07 May 2018). All efforts were made to minimize animal suffering and reduce the number of animals used.
Xu M., Ma Q., Fan C., Chen X., Zhang H, & Tang M. (2019). Ginsenosides Rb1 and Rg1 Protect Primary Cultured Astrocytes against Oxygen-Glucose Deprivation/Reoxygenation-Induced Injury via Improving Mitochondrial Function. International Journal of Molecular Sciences, 20(23), 6086.
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7

Sleep Restriction in ICR Mice

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All experiments were conducted following the Guide for the Care and Use of Laboratory Animals published by the Animal Welfare Committee of the Agricultural Research Organization, China Agricultural University (approval no. AW18079102-2). The origin, sex, and adaptation process of animals were the same as those described earlier [35 (link)]. Briefly, male ICR mice (8 weeks of age; Vital River Laboratory Animal Technology Co. Ltd., Beijing, China) were prepared for the experiment. After a week of adaptation, mice were divided into the sleep restriction (SR) and control (CON) groups.
The device and method of sleep restriction were the same as we used before [36 (link)]. The sleep restriction of the mice was from 12:00 p.m. to 8:00 a.m. the following day, and the sleep deprivation treatment was canceled within 4 h from 8:00 a.m. to 12:00 p.m. The treatment lasted 4 weeks. We set light-on time 7:00 a.m. as ZT0.
Li W., Wang Z., Cao J., Dong Y, & Chen Y. (2022). Role of Sleep Restriction in Daily Rhythms of Expression of Hypothalamic Core Clock Genes in Mice. Current Issues in Molecular Biology, 44(2), 609-625.
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8

Lethal Dose of IMB-0523 in Mice

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ICR mice (weight = 18–22 g; age = 4 weeks; n = 6 mice per group; 3 males, 3 females) were purchased from Vital River Laboratories (Beijing, China). Mice were injected with 500, 385, 296, and 228 mg/kg IMB-0523 intraperitoneally in a single-dose experiment. Survival and body weight were monitored daily for 7 days. The median lethal dose (LD50) of IMB-0523 was calculated according to the Karber method. All animal experiments were performed in full compliance with the protocols approved by the Animal Ethics Committee of the Institute of Medicinal Biotechnology (Beijing, China). All procedures were conducted in accordance with the guidelines of the Institutional Animal Care and Use Committees of the Chinese Academy of Medical Sciences.
Cui A.L., Sun W.F., Zhong Z.J., Jin J., Xue S.T., Wu S., Li Y.H, & Li Z.R. (2020). Synthesis and Bioactivity of N-(4-Chlorophenyl)-4-Methoxy-3-(Methylamino) Benzamide as a Potential Anti-HBV Agent. Drug Design, Development and Therapy, 14, 3723-3729.
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9

Pathogen-free Housing of ICR Mice

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ICR mice, obtained from Beijing Vital River Laboratory Animal Technology Co., Ltd., were housed under specific pathogen-free conditions in individual ventilated cage. The animals were raised and cared for according to the guidelines of the National Science Council of the Republic of China.
Wang H., Guo T., Yang Y., Yu L., Pan X, & Li Y. (2019). Lycorine Derivative LY-55 Inhibits EV71 and CVA16 Replication Through Downregulating Autophagy. Frontiers in Cellular and Infection Microbiology, 9, 277.
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10

Pharmacokinetics of NZX in Mice

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The 6-week-old, specific-pathogen-free, female ICR mice (starting weight, 23 to 27 g) were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd (Beijing, China). The animals were acclimatized for 2 to 3 days before the experiment. The pharmacokinetics of NZX was evaluated in uninfected female ICR mice that received a single tail vein NZX dose of 5, 10, or 20 mg/kg, respectively. After NZX administration, blood samples were collected by orbital venous from five mice per time point at 0.083 to 2 h intervals over 6 h. After centrifugation at 4,000 rpm for 20 min at 4°C, the serum was immediately separated and frozen at −80°C until analysis. Analyses of total serum concentrations of NZX were conducted by a microbiologic assay using S. aureus ATCC 6538p (CICC 10307) as the test strain (Andes et al., 2009 (link)). The developed microbial method proved to be simple, precise, and stable. The limit of quantification (LOQ) and detection (LOD) in the microbiologic assay were 0.5 μg/ml and 0.25 μg/ml, respectively. The protein binding of NZX in serum was measured by the ultrafiltration method using Centrifree Filters (Merck Millipore Ltd., Product code: 4104), according to previously described methods by Brinch et al. (2009 (link)). The concentration of NZX in the ultrafiltrate was determined by HPLC methods.
Zheng X., Yang N., Mao R., Hao Y., Teng D, & Wang J. (2022). Pharmacokinetics and Pharmacodynamics of Fungal Defensin NZX Against Staphylococcus aureus-Induced Mouse Peritonitis Model. Frontiers in Microbiology, 13, 865774.
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