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Iview dab detection kit

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The IVIEW DAB Detection Kit is a laboratory reagent used for the detection and visualization of target proteins in biological samples. The kit provides the necessary components for a chromogenic immunohistochemical staining procedure, allowing for the identification and localization of specific proteins within tissue sections or cell preparations.

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Lab products found in correlation

Benchmark xt, by Roche (31 mentions) Bond max, by Leica (9 mentions) Benchmark ultra, by Roche (5 mentions) Hematoxylin, by Roche (5 mentions) Glial fibrillary acidic protein (gfap), by Agilent Technologies (5 mentions) Ventana benchmark xt, by Roche (4 mentions) Protease 1, by Roche (4 mentions) Bluing reagent, by Roche (4 mentions) Benchmark ultra platform, by Roche (4 mentions) Pathvysion her2 dna probe kit, by Abbott (4 mentions) Tissue ia image analysis software, by Leica (4 mentions) Scn400 slide scanner, by Leica (4 mentions) Ventana benchmark, by Roche (3 mentions) Bond polymer refine detection kit, by Leica (3 mentions) Ventana benchmark ultra, by Roche (3 mentions) Cell conditioning solution cc1, by Roche (3 mentions) Cc1 solution, by Roche (3 mentions) Protease 2, by Roche (3 mentions) Nanozoomer scanner, by Hamamatsu Photonics (3 mentions) Nanozoomer digital pathology system ndpview, by Hamamatsu Photonics (3 mentions)

Spelling variants of this product

DAB detection kit (57 citations) Ventana iView DAB Detection Kit (7 citations) IView DAB (4 citations) DAB kit (4 citations) IView Universal DAB Detection kit (4 citations) IVIEW 3,3-diaminobenzidine (DAB) detection kit (3 citations) IView DAB Map detection kit (2 citations)

139 protocols using iview dab detection kit

1

Quantification of T Cells in Stroke Infarcts

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Histochemical and immunohistochemical staining was performed on sections cut to 6 µm from paraffin-embedded tissue blocks. Randomly chosen sections of every patient were deparaffinated and rehydrated and subsequently stained with H&E. Immunohistochemistry for CD3 (polyclonal rabbit anti-human CD3, diluted 1:50, A0452; Dako) was performed on sections adjacent to H&E-stained sections with the Ventana Benchmark GX automated staining system using a CC1 (Roche) pretreatment and the iView DAB Detection Kit (Roche). Sections were counterstained with hematoxylin and coverslipped with Entellan (Merck) as mounting medium. Age of infarcts was estimated by two experienced and independent neuropathologists (T. Arzberger and P.T. Nelson) on H&E sections according to published criteria (Mena et al., 2004 (link)). CD3 stains were scanned with Zeiss Axio Scan Z1 using a 20× objective. The infarct area was demarcated, and the absolute numbers and coordinates of intra- and extralesional CD3+ T cells were assessed manually using Qupath (version 0.2.2). Cell density was calculated as number of cells per cm2 of defined lesion area. The R index for quantification of cell clustering per area was calculated as described above in R (version 3.6.0). Intralesional cell density was correlated with time after stroke onset for each sample.
Heindl S., Ricci A., Carofiglio O., Zhou Q., Arzberger T., Lenart N., Franzmeier N., Hortobagyi T., Nelson P.T., Stowe A.M., Denes A., Edbauer D, & Liesz A. (2021). Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies. The Journal of Experimental Medicine, 218(8), e20202411.
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2

Immunohistochemical Pepsin Detection in Laryngeal Tissue

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Biopsy specimens of the laryngeal tissue were obtained during microlaryngoscopy procedures. Paraffin-embedded sections (2–3-μm thick) were prepared from the biopsy samples (Ventana Medical Systems, AZ, USA) and analyzed at the Department of Pathology by a single pathologist. Immunohistochemical analysis was performed after endogenous peroxidase blocking with hydrogen peroxide (Ventana) and antigen revitalization in CC1 buffer (Ventana). A pepsin antibody (NB100-66518, Novus Biologicals, CO, USA, diluted at a ratio of 1:100) was used as the primary antibody to detect pepsin. The incubation period for the primary antibody was 32 min. The iView DAB Detection Kit (Roche, Switzerland) was used to visualize the antigens. The presence of any antibody positivity in the cytoplasm of the cells was considered to be pathological and the sample was evaluated as pepsin positive.
Formánek M., Jančatová D., Komínek P., Tomanová R, & Zeleník K. (2017). Comparison of Impedance and Pepsin Detection in the Laryngeal Mucosa to Determine Impedance Values that Indicate Pathological Laryngopharyngeal Reflux. Clinical and Translational Gastroenterology, 8(10), e123-.
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3

Cytospin-based Cell Staining Protocol

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Immediately after sorting, 10,000 cells in 15 µl PBS buffer were added to a Cytospin carrier and subjected to centrifugation onto glass slides (80 g for 3 min). Slides were then air-dried and stained either next day or stored at −20 C until fixation and staining. The slides were subjected to routine haematoxylin-eosin (HE) staining. In short, slides of cells were briefly stained with haematoxylin and then by eosin solution, followed by alcohol dehydration. Some cells were also stained for peroxidase activity with the iVIEW DAB detection kit (Roche) according to the manufacturer’s instructions.
Guslund N.C., Solbakken M.H., Brieuc M.S., Jentoft S., Jakobsen K.S, & Qiao S.W. (2020). Single-Cell Transcriptome Profiling of Immune Cell Repertoire of the Atlantic Cod Which Naturally Lacks the Major Histocompatibility Class II System. Frontiers in Immunology, 11, 559555.
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4

Pediatric Brain Tumor Biopsy Protocol

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The Mayfield cranial stabilization and fixation system manufactured by Integra (Cincinnati, OH) is used to position the pediatric patient for surgical biopsy. The Stealth Vertek biopsy device, manufactured by Medtronic (Minneapolis, MN) is employed to collect specimens. Eight cores are typically acquired, as described in the Results section. Each needle core biopsy collected for cell culture and direct orthotopic implants is stored in RPMI (containing 500 mL RPMI-1640 with L-glutamine, and no phenol red (ThermoFisher cat.# 11835-030), 50 mL aliquot fetal calf serum (HyClone SH30070.03), 5 mL penicillin-streptomycin solution (ThermoFisher 15140-122), and 5 mL gentamicin (Sigma G1272)) at 4°C until ready for continued processing. Standard hematoxylin and eosin (H&E) staining is performed on primary biopsy tissue to assess tumor cell morphology. Staining for the diagnostic H3 K27M protein is performed with a 1:1200 antibody dilution (EMD Millipore cat.# ABE419) using the iView DAB detection kit (Roche cat. #760-091) on the Ventana Benchmark Ultra automated platform.
Biery M.C., Noll A., Myers C., Morris S.M., Winter C.A., Pakiam F., Cole B.L., Browd S.R., Olson J.M, & Vitanza N.A. (2020). A Protocol for the Generation of Treatment-naïve Biopsy-derived Diffuse Intrinsic Pontine Glioma and Diffuse Midline Glioma Models. Journal of experimental neurology, 1(4), 158-167.
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5

Immunohistochemical Profiling of Immune Markers

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The following primary antibodies and dilutions were used for immunohistochemistry: CD3 (1:100, rat monoclonal SP7, DCS Innovative Diagnostics, CI597R06), CD8+ (1:200, mouse monoclonal C8/144B, M710301-2), HDAC1 (ready to use, rabbit polyclonal, ABCAM, ab15316) and pan-cytokeratin (1:250, mouse monoclonal AE1/AE3, DAKO, M3515). The tissue sections were pre-treated with EDTA-buffer solution (pH 8.6; Cell Conditioning Solution, Roche, 950-124) at 95°C for 36 min. Immunohistochemical stainings were performed on an automated immunostainer according to the manufacturer's instructions (Ventana Medical Systems Inc.., Tucson, AZ, USA) using the iView DAB detection kit (Roche, 760-091) for single stain protocols. For double staining of HDAC1 and pan-cytokeratin, Ultraview universal alkaline phosphatase red detection kit (Roche, 760-501) was used in a second step.
Peper J.K., Bösmüller H.C., Schuster H., Gückel B., Hörzer H., Roehle K., Schäfer R., Wagner P., Rammensee H.G., Stevanović S., Fend F, & Staebler A. (2015). HLA ligandomics identifies histone deacetylase 1 as target for ovarian cancer immunotherapy. Oncoimmunology, 5(5), e1065369.
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6

Evaluating Tumor Immune Profiles via IHC

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Immune profiles (hot, cold, or excluded) were assigned after H&E evaluation by a board-certified pathologist. For 10 cases from each group, we performed IHC on 4-μm-thick sections of a formalin-fixed paraffin-embedded (FFPE) specimen after deparaffinization. We employed the IView DAB Detection Kit on a Ventana BenchMark (Roche, Basel, Switzerland). Immunostaining was performed followed by microwave-based antigen retrieval as previously described (29 (link)).
The following antibodies were used:
Idel C., Ribbat-Idel J., Klapper L., Krupar R., Bruchhage K.L., Dreyer E., Rades D., Polasky C., Offermann A., Kirfel J., Perner S, & Wollenberg B. (2021). Spatial Distribution of Immune Cells in Head and Neck Squamous Cell Carcinomas. Frontiers in Oncology, 11, 712788.
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7

Immunohistochemical and MSI Analysis of PDACs

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IHC staining of PDACs were performed using the standard streptavidin-biotin-peroxidase procedure. MMR IHC antibodies included MLH1 (Ventana, clone M1), MSH2 (Cell Marque, clone G219.1129), MSH6 (Ventana, clone 44), and PMS2 (BD Biosciences, clone A16.4). Detection systems were Bond Polymer Refine Detection (Newcastle Upon Tyne, UK) for MLH1 and PMS2, and iVIEW DAB Detection Kit (Roche Diagnostics, Indianapolis, IN) for MSH2 and MSH6. Tumors showing total absence of nuclear staining, with adjacent benign tissue showing nuclear staining, were scored as negative for expression of that protein. An experienced pathologist at MSKCC (JS) interpreted the histopathology and IHC results.
MSI analysis was performed using either the five microsatellite loci (BAT-25, BAT-26, NR-21, NR-24, and MONO-27) by PCR reaction or MSIsensor analysis. With MSI PCR testing, fluorescently labeled products were detected and sized by capillary electrophoresis. Microsatellite instability at ≥ 2 loci was defined as MSI-H, instability at a single locus was defined as MSI-L, and no instability at any of the loci tested was defined as MSS. MSIsensor interrogated the length distribution of all genomic microsatellite loci included in the MSK-IMPACT capture region across tumor and matched normal(12 (link)). MSI-H was defined as >10% of the microsatellite loci showing microsatellite instability.
Hu Z.I., Shia J., Stadler Z.K., Varghese A.M., Capanu M., Salo-Mullen E., Lowery M.A., Diaz LA J.r., Mandelker D., Yu K.H., Zervoudakis A., Kelsen D.P., Iacobuzio-Donahue C.A., Klimstra D.S., Saltz L.B., Sahin I.H, & O’Reilly E.M. (2018). Evaluating Mismatch Repair Deficiency in Pancreatic Adenocarcinoma: Challenges and Recommendations. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(6), 1326-1336.
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8

Immunohistochemical Detection of Erythrocytes

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To detect erythrocytes, the paraffin sections were incubated with anti-glycophorin A antibody JC 159 (Mouse Monoclonal Antibody, ref: Mob 066-05, Diagnostic BioSystems, Nanterre, France) at a 1/500 dilution using a Ventana Benchmark autostainer (Ventana Medical Systems, Inc., Tucson, AZ). Briefly, after removing the paraffin and washing them with reaction buffer, the sections were incubated with a primary antibody for 32 minutes at room temperature and then incubated with the reagent from an iVIEW DAB detection kit (Roche Diagnostics, Meylan, France). The sections were counterstained with hematoxylin and post-counterstained with bluing reagent. A negative control incorporating an irrelevant monoclonal antibody was run in parallel. The other paraffin sections were stained with HPS on a Tissue-Tek Prisma autostainer (Sakura, CA, US) and microscopically examined (DM2500 microscope Leica, Vienna, Austria).
Barbieri R., Mai B.H., Chenal T., Bassi M.L., Gandia D., Camoin-Jau L., Lepidi H., Aboudharam G, & Drancourt M. (2020). A 2,000–year-old specimen with intraerythrocytic Bartonella quintana. Scientific Reports, 10, 10069.
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9

Automated Immunohistochemistry Staining of Tissue Sections

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Formalin-fixed, paraffin-embedded 5 μm tissue sections were stained in batches for CD4, CD8, and CD163 in a central laboratory at the Johns Hopkins Hospital according to standard automated protocols. Deparaffinization and rehydration were performed, followed by antigen retrieval and antibody staining. CD4 and CD8IHC was performed using the Ventana Benchmark Ultra autostaining system (Roche) using mouse monoclonal anti-CD8 (C8144B) antibody (Cell marque) and rabbit monoclonal anti-CD4(Sp35) antibody (Roche), followed by detection with the iVIEW DAB Detection Kit (Roche). CD163 IHC was performed on the Leica Bond MAX autostaining system (Leica Biosystems) using anti-CD163 (10D6) antibody (Leica Biosystems) followed by detection with Bond Polymer Refine Detection kit (Leica Biosystems). For tissue section imaging, slides were imaged using a Ventana iScan HT slide scanner (Roche) and processed using the Ventana Virtuoso software (Roche) (Figure S6D).
Clark D.J., Dhanasekaran S.M., Petralia F., Pan J., Song X., Hu Y., da Veiga Leprevost F., Reva B., Lih T.S., Chang H.Y., Ma W., Huang C., Ricketts C.J., Chen L., Krek A., Li Y., Rykunov D., Li Q.K., Chen L.S., Ozbek U., Vasaikar S., Wu Y., Yoo S., Chowdhury S., Wyczalkowski M.A., Ji J., Schnaubelt M., Kong A., Sethuraman S., Avtonomov D.M., Ao M., Colaprico A., Cao S., Cho K.C., Kalayci S., Ma S., Liu W., Ruggles K., Calinawan A., Gümüş Z.H., Geiszler D., Kawaler E., Teo G.C., Wen B., Zhang Y., Keegan S., Li K., Chen F., Edwards N., Pierorazio P.M., Chen X.S., Pavlovich C.P., Hakimi A.A., Brominski G., Hsieh J.J., Antczak A., Omelchenko T., Lubinski J., Wiznerowicz M., Linehan W.M., Kinsinger C.R., Thiagarajan M., Boja E.S., Mesri M., Hiltke T., Robles A.I., Rodriguez H., Qian J., Fenyö D., Zhang B., Ding L., Schadt E., Chinnaiyan A.M., Zhang Z., Omenn G.S., Cieslik M., Chan D.W., Nesvizhskii A.I., Wang P, & Zhang H. (2019). Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma. Cell, 179(4), 964-983.e31.
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10

Immunohistochemical Staining of CDK7 and pMED1

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Immunohistochemical staining was performed on 4 μm thin sections of FFPE tissue. Sections were deparaffinized and antigens were heat-mediated retrieved, as previously described [43 (link)]. Automated staining was applied using the automated Ventana BenchMark staining system and the IViewDAB Detection Kit (both Roche, Basel, Switzerland). For the staining of CDK7 and pMED1, the following antibodies were used at the indicated dilution after successful control tissue staining according to data sheets: CDK7 (mouse monoclonal, CDK7 (MO1) Mouse mAb, 1:500, Cell Signaling, Danvers, MA, USA) and pMED1 (rabbit polyclonal, Anti-TRAP220/MED1 (phospho T1457) antibody, 1:100, Abcam, Cambridge, UK). Slides were then counterstained with hematoxylin and bluing reagent.
Jagomast T., Idel C., Klapper L., Kuppler P., Offermann A., Dreyer E., Bruchhage K.L., Ribbat-Idel J, & Perner S. (2022). CDK7 Predicts Worse Outcome in Head and Neck Squamous-Cell Cancer. Cancers, 14(3), 492.
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