One normal human breast epithelial cell line (MCF-10A) and four BC cell lines (MDA-MB-231, MDA-MB-468, MCF7 and MDA-MB-415) were obtained from the Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China). These cells were cultured in Dulbecco's modified Eagle's medium (DMEM; Gibco-BRL, Grand Island, New York, NY, USA) supplemented with 10% fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, MO, USA). All the cells were maintained at 37°C in a humidified incubator with 5% CO2.
Mcf 7
Manufactured by Shanghai Cell Bank
Sourced in China
MCF-7 is a cell line derived from a human breast adenocarcinoma. It is a commonly used cell line in cancer research and drug development.
Automatically generated - may contain errors
Lab products found in correlation
Mda mb 231, by Shanghai Cell Bank (39 mentions)
Mcf 10a, by Shanghai Cell Bank (19 mentions)
Sk br 3, by Shanghai Cell Bank (16 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (16 mentions)
Mda mb 468, by Shanghai Cell Bank (12 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (9 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (9 mentions)
Bt 549, by Shanghai Cell Bank (8 mentions)
Mda mb 453, by Shanghai Cell Bank (7 mentions)
Penicillin, by Merck Group (6 mentions)
Streptomycin, by Merck Group (6 mentions)
Trypsin, by Thermo Fisher Scientific (6 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (5 mentions)
Bt474, by Shanghai Cell Bank (4 mentions)
Fetal bovine serum (fbs), by Merck Group (4 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (4 mentions)
Mcf 7, by Thermo Fisher Scientific (3 mentions)
Hepg2, by Shanghai Cell Bank (3 mentions)
Smmc 7721, by Shanghai Cell Bank (3 mentions)
Dmem medium, by Thermo Fisher Scientific (3 mentions)
65 protocols using mcf 7
1
Breast Cancer Cell Line Culture
2
Coculture of Human Breast Cancer Cells and Fibroblasts
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Human embryonic skin fibroblasts (ESF) were purchased from the Institute of Basic Medicine, Chinese Academy of Medical Sciences (Beijing, China); human breast cancer cell lines (MAD-MB231 and MCF-7) were purchased from Shanghai Cell Bank, Chinese Academy of Sciences (Shanghai, China).
MCF-7 or MAD-MB231 cells (5×10
5) were seeded on a 6-well plate in 2 mL complete media (DMEM, Hyclone, USA) supplemented with 10% FBS (ExCell Bio, Shanghai, China) and 1% penicillin/streptomycin, and fibroblasts (2×10
5) were seeded on the 0.4 mm polyester membrane of a 6-mm Transwell insert (Corning, Lowell, USA) in 2 mL complete media and placed in a separate culture plate. After 24 h, Transwell insert was subsequently transferred to the culture plate with MCF-7 or MDA-MB-231 cells, and the cells were cultured in 2 mL of complete medium at 37°C in a humidified incubator containing 5% CO
2. The up and down position of human breast cancer cell lines and ESF cells can be switched according to subsequent experiments.
MCF-7 or MAD-MB231 cells (5×10
5) were seeded on a 6-well plate in 2 mL complete media (DMEM, Hyclone, USA) supplemented with 10% FBS (ExCell Bio, Shanghai, China) and 1% penicillin/streptomycin, and fibroblasts (2×10
5) were seeded on the 0.4 mm polyester membrane of a 6-mm Transwell insert (Corning, Lowell, USA) in 2 mL complete media and placed in a separate culture plate. After 24 h, Transwell insert was subsequently transferred to the culture plate with MCF-7 or MDA-MB-231 cells, and the cells were cultured in 2 mL of complete medium at 37°C in a humidified incubator containing 5% CO
2. The up and down position of human breast cancer cell lines and ESF cells can be switched according to subsequent experiments.
3
Culturing Breast Cell Lines
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Normal breast epithelial cell MCF-10A and breast cancer cell lines SK-BR-3, MCF-7, MDA-MB-231, and MDA-MB-468 were purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences. For the experiment, the cells were first thawed and then re-suspended in DMEM medium containing +1% penicillin-streptomycin solution +10% fetal bovine serum. The cells were transferred to culture dishes and observed at 37°C and 5%CO2.
4
Cell Culture Protocol for Breast Cancer Lines
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MCF‐7, SK‐BR‐3, MDA‐MB‐231, and MDA‐MB‐453 cell lines were obtained from Shanghai Cell Bank. All cells were cultured in RPMI‐1640 medium (Invitrogen) containing 10% FCS (Invitrogen), 100 IU/mL penicillin, and 100 μg/mL streptomycin (Sigma‐Aldrich). The cells were grown in sterile culture dishes at 37°C in a 5% CO2 atmosphere and passaged every 2 days, using 0.25% trypsin (Invitrogen) for cell detachment. All cell lines were authenticated using short tandem repeat DNA profiling.
5
Characterization of Breast Cell Lines
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The non-tumorigenic mammary epithelial cell line MCF-10A, and the breast cancer cell lines MCF-7, MDA-MB-231, SK-BR-3, and MDA-MB-453 were obtained from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and identified by short tandem repeat (STR) DNA analysis. MCF-10A cells were cultured in 1:1 Dulbecco’s modified Eagle’s medium (DMEM)/F12 (Gibco, Waltham, MA, USA) supplemented with 5% serum, 10μg/mL insulin (Sigma-Aldrich Co, St. Louis, MO, USA), and 20 ng/mL epidermal growth factor (EGF). MCF-7, SK-BR-3 and MDA-MB-453 cells were cultured in DMEM (Gibco, Waltham, MA, USA) supplemented with fetal bovine serum (FBS). MDA-MB-231 cells were cultured in L15 (Gibco, Waltham, MA, USA) supplemented with 10% FBS. All cells were cultured at 37°C in a humidified incubator with 5% CO2.
6
Establishment and Maintenance of Cancer Cell Lines
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The human breast cancer cell lines MDA-MB-231, MCF-7 and T-47D, human hepatocellular carcinoma (HCC) cell lines HepG2 and SMMC7721, human nasopharyngeal carcinoma cell lines HNE1 and CNE-2Z, human gastric cancer cell line SGC7901, human endometrial cancer cell line ISK, and human non-small cell lung cancer cell line A549 were bought from Shanghai Cell Bank (Shanghai, China). The human colon carcinoma SW480 was obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were obtained, frozen and cultured in our laboratory. The cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) or Roswell Park Memorial Institute (RPMI)-1640 medium (Gibco), supplemented with 10 % fetal bovine serum (FBS), 100 U/mL penicillin, and 100 μg/mL streptomycin. Cells were grown in an atmosphere containing 5%CO2 at 37 °C.
7
Breast Cancer Cell Culture Protocols
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Breast cancer cell lines MDA-MB-231, MCF-7 and 4T1 were purchased from the Shanghai Cell Bank located in the Chinese Academy of Science (Shanghai, China). MDA-MB-231 cells were cultured in DMEM high glucose complete medium, containing 10% foetal bovine serum and 1% penicillin-streptomycin. MCF-7 and 4T1 cells were cultured using RPMI 1640 complete medium containing 10% foetal bovine serum and 1% penicillin-streptomycin. Cells were cultured under conditions of 5% CO2 at 37 °C.
8
Culturing Human Breast Cancer Cells
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Human breast cancer cell lines Michigan Cancer Foundation (MCF)-7 and MDA-MB-231 were purchased from the Chinese Academy of Sciences Shanghai Cell Bank. The cells were cultured in Roswell Park Memorial Institute (RPMI) 1640 complete medium (Gibco, Carlsbad, California, USA) at 37 °C in an incubator containing 5% CO2. The complete medium comprised 10% fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (100 μg/ml).
9
Cell Culture Conditions for Cancer
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Breast cancer MCF-7, MBA-MB-435, and T47D cells and lung cancer A549, H460, and Calu-1 cells were obtained from the Shanghai Cell Bank (China). All cells were cultured in a 5% CO2 incubator (with a HEPA class 100 filter) in RPMI 1640 or DMEM medium of 100 U/mL of penicillin-streptomycin and 10% fetal bovine serum. Medium was replaced three times a week. Cells were used during the exponential growth phase of all experiments.
10
Cell Culture Protocols for Various Cell Lines
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293T, Raji, SU-DHL-4, Daudi, Nalm6, Jurkat, Panc-1,U937, PC-3, and MCF-7 cell lines (Shanghai Cell Bank). High glucose DMEM (SH30022.01B, Hyclone) was used to culture the 293T, Panc-1, and MCF-7 cell line. The remaining hematological tumor cell lines were cultured in RPMI-1640 medium (SH30809.01B, Hyclone). All cell lines were incubated at 37°C with 5% CO2.
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