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119 protocols using ms excel

1

Quantitative Analysis of Fly Tissues

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Experiments were performed at least in duplicates (for each biological replicate, n ≥ 2). Assays were done after pooling isolated male/female somatic tissues from 10 to 20 flies, unless otherwise stated. For statistical analysis, the MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 19.0 for Windows, NY, USA) were used. Statistical significance was evaluated using one-way analysis of variance (ANOVA). Data points correspond to the mean of the independent experiments and error bars denote standard deviation (SD); significance at P < 0.05 or P < 0.01 is indicated in graphs by one or two asterisks, respectively. For LC-MS experiments (n = 3) differences in Log2 ratio values at P < 0.05 were accepted.
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2

Experimental Statistical Analysis Protocol

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Experiments were performed at least in duplicate unless otherwise indicated in figure legends. For statistical analyses, MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 19.0 for Windows) were used. Statistical significance was evaluated using one-way analysis of variance (ANOVA). Data points correspond to the mean of the independent experiments and error bars denote standard deviation (SD).
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3

Statistical Analysis of Qualitative and Quantitative Data

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Chi-square test was used as test of significance for qualitative data. Independent t-test was used as test of significance to identify the mean difference between two quantitative variables. P (probability that the result is true) < 0.05 was considered as statistically significant after assuming all the rules of statistical tests. Statistical software: MS Excel, SPSS version 22 (IBM SPSS Statistics, Somers NY, USA) was used to analyze data.
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4

Phosphoproteomic and Cytokine Secretion Analysis

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Experiments were performed at least in duplicates, and shown data points correspond to the mean of independent experiments. Statistical analysis was performed by using the MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 19.0 for Windows); significance was evaluated using one‐way analysis of variance (ANOVA). Error bars indicate standard deviation (SD); significance at P < .05 or P < .01 is indicated in graphs or heatmaps by one or two asterisks, respectively. Significance for the phosphoproteomic and cytokine/chemokine secretion set of experiments was estimated as a combination of median fluorescence intensity (MFI) value above 600 and fold change (FC) value above 0.3 when compared to control samples.
Additional methods are available in Supporting Information.
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5

Statistical Analysis of Experimental Data

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Experiments were performed at least in duplicates unless otherwise indicated in figure legends. For statistical analyses MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 19.0 for Windows) were used. Statistical significance was evaluated using one-way analysis of variance (ANOVA). Data points correspond to the mean of the independent experiments and error bars denote standard deviation (SD.); significance at P<0.05 or P<0.01 is indicated in graphs by one or two asterisks, respectively.
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6

Statistical Analysis of Experimental Data

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Experiments were performed in duplicates or triplicates as indicated in Figure legends. For statistical analyses MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 19.0 for Windows, Armonk, NY, USA) were used. Statistical significance was evaluated using one-way analysis of variance (ANOVA). Data points correspond to the mean of the independent experiments and error bars denote standard deviation (SD).
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7

Statistical Analysis of Quantitative Data

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Data was analyzed using the Statistical Package for the Social Sciences (SPSS) 22 version software from Microsoft excel data sheet. Categorical data was represented in the form of proportions and frequencies. Qualitative data for the test of significance was done by utilizing Chi-square test or Fischer's exact test (for 2 × 2 tables only).
Continuous data were represented as standard deviation and mean. To identify the mean difference between the two quantitative variables, an independent t test was used as a test of significance. Graphical representation of data, MS Excel and MS word was used to obtain various types of graphs. P value (probability that the result is true) of <0.05 was considered as statistically significant after assuming all the rules of statistical tests. Statistical software: MS Excel, SPSS version 22 (IBM SPSS Statistics, Somers NY, USA) was used to analyze data.
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8

Statistical Analysis of Soft Benefits

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Data entry was done by using Ms-Excel (2016) and for descriptive and inferential statistics IBM SPSS V. 20 was used. However, the calculation of soft benefit was done by using the formula suggested by (Tomalty et al., 2010) and cited by (Kumar et al., 2019; (link)Tomalty et al., 2010) . The details of the calculation of soft benefits are as follows:
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9

Statistical Analyses of Biological Replicates

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Experiments were performed at least in duplicates (biological replicates). For statistical analyses the MS Excel and the Statistical Package for Social Sciences (IBM SPSS; version 19.0 for Windows) were used. Statistical significance was evaluated using one-way analysis of variance (ANOVA). Data points correspond to the mean of the independent experiments and error bars denote standard deviation (S.D.); significance at P < 0.05 or P < 0.01 is indicated in graphs by one or two asterisks, respectively. For flies' longevity/survival curves and statistical analyses, the Kaplan-Meier procedure and log-Rank (Mantel-Cox) test were used (see Table S3).
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10

Statistical Analysis of Cross-Sectional Survey Data

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Data obtained from the survey were analyzed using MS Excel and SPSS software. The cross-sectional study of this research involved both descriptive and inferential methods. The descriptive analysis consisted of frequency, percentage, cumulative percentage, etc. The Cross-Tabulation technique was employed to display a breakdown of the data. A chi-square test for independence also called Pearson’s chi-square test of association was used to determine whether there was a significant relationship between two categorical variables (Franke et al. 2012 (link); Rana 2015 (link)). The chi-square test was calculated by using the formula,
χn=OiEi2Ei
With a degree of freedom (DF) = (r-1) × (c-1), where c denotes the number of levels for one categorical variable, and r denotes the number of levels for the other categorical variable. Likewise, Oi represents observed frequency and Ei represents the expected frequency under the null hypothesis which was computed separately for each level of one categorical variable at each level of the other categorical variable. Based on the test statistics and the DF, the P-values were also determined at a 0.05 level of significance.
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