Trace 1300 series gc

The ACEOs from rhizome and leaf were analyzed by Thermo Scientific™ TRACE™ 1300 Series GC operated with a split mode injector, Thermo Scientific AI/AS 1310 Series autosampler, and Thermo Scientific™ ISQ™ Series GC-Single Quadrupole MS. The following were the specifications used for analysis: column: TG Wax MS (acid-deactivated polyethylene glycol) 30 m, 0.25 mm i.d., 0.25 μm film thickness (Thermo Scientific, USA); temperature program: from 60°C to 150°C at 3°C/min and from 150°C to 240°C at 7°C/min; injector temperature: 240°C; injection volume: 1.0 μL; inlet pressure: 86.3 kPa; carrier gas: He; flow rate: 1.000 mL/min; injection mode: split (50 : 1); mass interface temp.: 250°C; MS mode: EI; detector voltage: 70 eV; mass range: 40–450; and interval: 0.2 sec. Data handling was made through Xcalibur software. The relative amount of individual components of the total oil is expressed as percentage peak area relative to total peak area. Qualitative identification of the different constituents was performed by comparison of their relative retention times and mass spectra with those of authentic reference compounds, or by retention indices (RI) and mass spectra. Compound identification was done by comparing the National Institute of Standards and Technology (NIST 2014) library data of the mass spectra peaks with those reported in the literature.

Gas chromatography-mass spectrometry (GC-MS) analyses of the samples were performed using a Thermo Scientific Trace 1300 Series GC coupled to a Thermo Scientific Quantum XLS Ultra MS. The GC capillary column used was a Phenomenex Zebron ZB–5MS (30 m, 0.1 µm film thickness, inner diameter 0.25 mm). Compounds were transferred splitless to the GC column at an injector temperature of 300 °C. Helium was used as the carrier gas at a flow rate of 1.5 mL/min. The GC temperature program was as follows: 80 °C (hold 1 min), 80 °C to 310 °C at 5 °C/min (hold 20 min). Electron ionization mass spectra were recorded at 70 eV electron energy in full scan mode (mass range m/z 50–600, scan time 0.42 s). Peak areas were integrated using Thermo Xcalibur software version 2.2 (Thermo Fisher Scientific Inc., Waltham, MA, USA).