Mkn28

The human GC cell lines (AGS, HGC27, MKN28, MKN45, and MGC-803) and normal human gastric epithelium (GES-1) were obtained from Shanghai Cell Bank and authenticated by STR before shipping. Mycoplasma contamination was tested if concerned. Cells were cultured in DMEM with 10% fetal bovine serum (Gibco, Grand Island, NY, USA). siRNAs targeting METTL3 were synthesized by GenePharma. The shRNAs targeting NDUFA4 or IGF2BP1 were cloned into a pLKO.1 vector and packaged as lentivirus. The sequences of shRNA and siRNAs were listed in Supplementary Table 1.

MKN28, SGC7901 along with BGC823 human cell lines were supplied by Cell Bank of Shanghai (Shanghai, China) and maintained in DMEM containing high glucose and enriched with 10% FBS inactivated with heat. The cells at the logarithmic phase were used in the experiments.

The 293 T cell and human GC cell lines (MKN28, BGC823 and SGC7901) were obtained from the Cell Bank of Shanghai (Shanghai, China) and cultured in Dulbecco's Modified Eagle Medium (DMEM, Life Technologies)/high glucose medium supplemented with 10% heat-inactivated newborn calf serum at 37°C in a humidified incubator under a 5% carbon dioxide atmosphere. All of the experiments were performed with cells in the logarithmic phase of growth.

Human GC cell lines (SGC‐7901, MGC‐803, MKN‐28 and BGC‐823) and a normal gastric epithelium cell line (GES‐1) were purchased from the Cell Bank of Shanghai Institute of Cell Biology, Chinese Academy of Medical Science (Shanghai, China). Cells were cultured in DMEM with 10% Gibco fetal bovine serum (Thermo Fisher Scientific, Waltham, MA, USA) with antibiotics (Sigma‐Aldrich, St. Louis, MO, USA) at 37 °C in 5% CO₂.
Precursor miRNA clones (mimic‐miR‐944), miR‐944 inhibitors (anti‐miR‐944), MACC1 over‐expression plasmid (pcDNA3.1‐MACC1) and corresponding negative control were designed and synthesized by GeneCopoeia (Guangzhou, China). MACC1 siRNA and a scrambled control siRNA were designed and synthesized by Shanghai GenePharma Co., Ltd (Shanghai, China). Cell transfection was performed by using Lipofectamine 2000 (Invitrogen/Thermo Fisher Scientific) according to the supplier's protocol.

Human gastric cancer cell lines (HGC-27, MGC-803, BGC-823, SGC-7901, and MKN-28) and human umbilical vein endothelial cells (HUVECs) were purchased from Cell Bank of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China). Gastric cancer cell lines were grown in Roswell Park Memorial Institute (RPMI) 1640 medium (Sigma-Aldrich, USA) that contained 10 % fetal bovine serum (FBS; HyClone, USA). HUVECs were maintained in DMEM medium containing 10 % FBS. All the media were supplemented with 100 U/ml penicillin and 100 μg/ml streptomycin (Invitrogen, USA) and maintained in 5 % CO₂ at 37 °C.