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Facsaria sorter

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The FACSAria sorter is a high-performance cell sorting instrument designed for cell analysis and separation. It utilizes flow cytometry technology to detect and sort individual cells based on their physical and fluorescent properties.

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Lab products found in correlation

Facsdiva software, by BD (10 mentions) Ter119, by BioLegend (5 mentions) Rpmi 1640, by Thermo Fisher Scientific (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions) Dnase 1, by Merck Group (4 mentions) Fortessa, by BD (4 mentions) Streptavidin conjugated microbeads, by Miltenyi Biotec (4 mentions) Accuri c6 analyzer, by BD (3 mentions) Cflow, by BD (3 mentions) Recombinant gm csf, by Thermo Fisher Scientific (3 mentions) Human monocyte nucleofection kit, by Lonza (3 mentions) Facscanto 2, by BD (3 mentions) Facscalibur, by BD (3 mentions) Accuri c6, by BD (3 mentions) Dxp11 analyzer, by Cytek (3 mentions) Anti cd8α macs beads, by Miltenyi Biotec (3 mentions) 7 aad, by BD (3 mentions) 7 aad, by Merck Group (3 mentions) F4 80, by BioLegend (3 mentions) Cd44 apc and cd24 pe, by BD (2 mentions)

Close spelling variants of this product

FACSAria (4019 citations) FACSAria II cell sorter (972 citations) FACSAria III cell sorter (895 citations) FACSAria II sorter (176 citations) FACSAria IIu sorter (15 citations) BD FACSAria III SORP cell sorter (14 citations) FACSAria II High-Speed Cell Sorter (13 citations) FACSAria™ Fusion high-speed cell sorter (6 citations) FACSAria IIu SORP cell sorter (6 citations) FACSAria II flow sorter (5 citations)

217 protocols using facsaria sorter

1

FACS Sorting of HMLE Stem-Like Cells

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Parental HMLE cells (1 × 107) were in incubated with CD44-APC and CD24-PE (BD Biosciences)-conjugated antibodies and placed on ice for 45 min then washed with blocking buffer (PBS with 1% FBS) twice. HMLE CD24highCD44high and CD24lowCD44high cells were sorted from parental HMLE by FACSAria sorter (BD Biosciences) for further experiments. After sorting, the purity of CD24highCD44low and CD24lowCD44high population was analyzed by Flow Cytometry (99% and 95%, respectively).
Liu Z., Zhang W., Phillips J.B., Arora R., McClellan S., Li J., Kim J.H., Sobol R.W, & Tan M. (2018). Immunoregulatory Protein B7-H3 Regulates Cancer Stem Cell Enrichment and Drug Resistance through MVP-mediated MEK Activation. Oncogene, 38(1), 88-102.
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2

Intracellular Lipid Quantification by Flow Cytometry

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Intracellular lipid content was evaluated via flow cytometry using 4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene (BODIPY 493/503; Life Technologies) (Herber et al., 2010 (link)). Briefly, 5×106 splenic or malignant peritoneal wash cells were stained for surface markers using antibodies that do not overlap with BODIPY 493/503, namely CD11c-APC, CD45-APC-Cy7, and CD11b-Pacific Blue, followed by staining with 500 μl of BODIPY 493/503 at 0.5 μg/ml in PBS for 15 min at room temperature in the dark. BODIPY 493/503 staining was detected in the PE channel. For intracellular cytokine staining, 5 × 106 cells from malignant peritoneal wash samples were stimulated for 6 hr in 10% FBS complete RPMI containing phorbol myristate acetate (PMA), Ionomycin (Calbiochem), and Brefeldin A (BioLegend). Cells were collected and stained for surface markers and intracellular cytokines (BioLegend) according to Foxp3/Transcription Factor Staining buffer set (eBioscience). Flow cytometry was performed on a LSRII instrument (BD Biosciences). Cell populations were sorted from peritoneal washes of OvCa-bearing mice or human ascites or tumor single-cell suspensions with a FACSAria sorter (BD Biosciences), and flow cytometry data were analyzed using FlowJo v.9 or v.10.
Cubillos-Ruiz J.R., Silberman P.C., Rutkowski M.R., Chopra S., Perales-Puchalt A., Song M., Zhang S., Bettigole S.E., Gupta D., Holcomb K., Ellenson L.H., Caputo T., Lee A.H., Conejo-Garcia J.R, & Glimcher L.H. (2015). ER Stress Sensor XBP1 Controls Anti-tumor Immunity by Disrupting Dendritic Cell Homeostasis. Cell, 161(7), 1527-1538.
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3

Lentiviral Lineage Tracing using RIP-Cre/ER and pTrip–loxP

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RIP-Cre/ER and pTrip–loxP-NEO-STOP-loxP-eGFP lentiviruses [3 (link)] were used for lineage tracing. Virus production, cell infection, and tamoxifen treatment were previously described [3 (link)]. eGFP-labeled cells were sorted using a FACS Aria sorter (BD Biosciences) as described [2 (link)].
Nathan G., Kredo-Russo S., Geiger T., Lenz A., Kaspi H., Hornstein E, & Efrat S. (2015). MiR-375 Promotes Redifferentiation of Adult Human β Cells Expanded In Vitro. PLoS ONE, 10(4), e0122108.
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4

Fluorescence-Activated Cell Sorting of Zebrafish CNS Cells

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Cell suspensions were sorted directly into 96 well plates, filled with culture medium only, or pre-seeded with motor neurons (MNs) using a BD FACSAria sorter. For the detection of GFP a 488 nm excitation laser and a 530/30 bandpass filter were used. DsRed was detected with a 582/15 bandpass filter after excitation with a 561 nm laser. VybrantTM DyeCycleTM Violet Stain was detected after 405 nm excitation and a 450/40 bandpass filter. Cellular events were defined by forward and side scatter profile and by the dsRed or GFP signal of the corresponding transgenic line. From this selection, all events that showed incorporation of the VybrantTM DyeCycleTM Violet Stain were gated because this proved to be an ideal selection to separate the small zebrafish CNS cells from remaining cellular and myelin debris. To assess purity and viability of the sorted cells post-sort analysis of the purified cells after addition of Propidium Iodide at a final concentration of 1 μg/mL was performed.
Kroehne V., Tsata V., Marrone L., Froeb C., Reinhardt S., Gompf A., Dahl A., Sterneckert J, & Reimer M.M. (2017). Primary Spinal OPC Culture System from Adult Zebrafish to Study Oligodendrocyte Differentiation In Vitro. Frontiers in Cellular Neuroscience, 11, 284.
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5

Sorting Mammary Epithelial Cell Subsets

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Mice used in this study have been previously described13 (link). YFP+ ER− luminal progenitor cells (LPs) were sorted from MMTV-Cre;Runx1f/f;R26Y and MMTV-Cre;Runx1+/+;R26Y (control) females (2 months of age) and YFP+ ER+ mature luminal cells (MLs) were sorted from MMTV-Cre;Runx1f/f;Rb1f/f;R26Y and MMTV-Cre;Runx1+/+;Rb1f/f;R26Y (control) females (either 2-month or 7-month old). R26Y is a conditional Cre-reporter that expresses YFP upon Cre-mediated recombination. In the compound mice, conditional knockout of Runx1 (and Rb1) in mammary epithelial cells is linked to YFP expression. Breeding with Rb1f/f mice facilitated rescue of the ER+ luminal cell subpopulation upon Rb1 deletion as described13 (link). FACS sorting was performed with a FACSAria sorter (BD Biosciences) using antibodies from eBiosciences including CD24-eFluor450, CD24-eFluor605, CD29-APC, c-Kit-PE-CY7, CD14-PE and biotinylated CD31, CD45, TER119 and Streptavidin-PerCP-CY5.5. All animal work was performed in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health and was approved by the Institutional Animal Care and Use Committee of Boston Children's Hospital where the animals are housed.
Chimge N.O., Little G.H., Baniwal S.K., Adisetiyo H., Xie Y., Zhang T., O'Laughlin A., Liu Z.Y., Ulrich P., Martin A., Mhawech-Fauceglia P., Ellis M.J., Tripathy D., Groshen S., Liang C., Li Z., Schones D.E, & Frenkel B. (2016). RUNX1 prevents oestrogen-mediated AXIN1 suppression and β-catenin activation in ER-positive breast cancer. Nature Communications, 7, 10751.
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6

Isolation and Characterization of Murine Epidermal Stem Cells

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Primary adult epidermal keratinocytes were isolated from the dorsal skin of 8- to 9-week old mice as previously described (42 (link)). Freshly isolated epidermal keratinocytes were suspended in 1% BSA-PBS and stained with FITC-conjugated CD49f-Integrin α6, PE-conjugated Ly-6A/E (Sca-1) and APC-conjugated CD34 antibodies (see below for details regarding dilutions). After washing, cells were stained with 4′,6-Diamidino-2-phenylindole dihydrochloride (DAPI) and then subjected to FACS analysis using a FACS Aria sorter and FACS DiVa 4.1 software (BD Biosciences, San Jose, CA, USA). In all cases, cells flew under 30 psi pressure through a 100 μm nozzle. After eliminating DAPI-positive dead cells, FITC, APC and PE signals were collected through 530/30, 660/20 and 576/26 nm band-pass filters, respectively. Viable α6-positive cells (PE+) were identified and further gated based on their surface expression of CD34 (APC+) and the absence of Sca-1 (FITC-).
Cabañero D., Irie T., Celorrio M., Trousdale C., Owens D.M., Virley D., Albrecht P.J., Caterina M.J., Rice F.L, & Morón J.A. (2016). Identification of an epidermal keratinocyte AMPA glutamate receptor involved in dermatopathies associated with sensory abnormalities. Pain Reports, 1(3), e573.
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7

Phospho-H2AX Quantification in T Cells

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Co-transferred live (PI-) WT and Hmgb2−/− P14 T cells were sorted at >95% purity from spleens and LNs of LCMV Arm or Cl13 infected mice at 8dpi using a BD FACSAria sorter. Cells were lysed and blots were stained for Phospho-H2AX (Ser139) (1:1000) and Histone H3 (1:140000).
Neubert E.N., DeRogatis J.M., Lewis S.A., Viramontes K.M., Ortega P., Henriquez M.L., Buisson R., Messaoudi I, & Tinoco R. (2023). HMGB2 regulates the differentiation and stemness of exhausted CD8+ T cells during chronic viral infection and cancer. Nature Communications, 14, 5631.
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8

Gal8-Expressing RAW 264.7 Macrophage Generation

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RAW 264.7 macrophages were obtained from ATCC and cultured in RPMI 1640 (Gibco) supplemented with 10% FBS (Gibco). For toxicity assays, macrophages were seeded at 15k cells/well in a 96-well plate. Cells were cultured with peptide or micelles for 24 hours and viability was determined by MTS/PMS (Promega) by plate reader. The Gal8-RAW 264.7 cell line was generated through similar means as described.28 (link) Briefly, RAW cells were co-transfected with plasmids containing a transposable Gal8-GFP construct and PiggyBac transposon (gift of Prof. Jordan Green) using Lipofectamine 2000 (3:1 molar ratio transposon:transposase plasmid). Cells were sorted for the top 5% brightest GFP+ singlet cell events using a FACS Aria sorter (BD), expanded, and sorted three more times to yield a population of Gal8-GFPhigh cells.
Sylvestre M., Lv S., Yang L.F., Luera N., Peeler D.J., Chen B.M., Roffler S.R, & Pun S.H. (2021). Replacement of L-amino acid peptides with D-amino acid peptides mitigates anti-PEG antibody generation against polymer-peptide conjugates in mice. Journal of controlled release : official journal of the Controlled Release Society, 331, 142-153.
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9

FACS Sorting of HMLE Stem-Like Cells

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Parental HMLE cells (1 × 107) were in incubated with CD44-APC and CD24-PE (BD Biosciences)-conjugated antibodies and placed on ice for 45 min then washed with blocking buffer (PBS with 1% FBS) twice. HMLE CD24highCD44high and CD24lowCD44high cells were sorted from parental HMLE by FACSAria sorter (BD Biosciences) for further experiments. After sorting, the purity of CD24highCD44low and CD24lowCD44high population was analyzed by Flow Cytometry (99% and 95%, respectively).
Liu Z., Zhang W., Phillips J.B., Arora R., McClellan S., Li J., Kim J.H., Sobol R.W, & Tan M. (2018). Immunoregulatory Protein B7-H3 Regulates Cancer Stem Cell Enrichment and Drug Resistance through MVP-mediated MEK Activation. Oncogene, 38(1), 88-102.
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10

Cell Sorting and Gene Expression Analysis

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Cryopreserved immune cells were thawed and stained with CD56 (NCAM-1) APC, CD3 (UCHT1) BUV395, and CD19 (SJ25C1) PE and sorted to high purity using a FACSAria sorter (BD, Franklin Lakes, NJ). Live cells were sorted into three groups: NK cells (CD56+, CD3-), B cells (CD19+, CD3-, CD56-), and T cells (CD3+, CD56-). RNA was isolated with a RNeasy Micro Kit Plus (Qiagen, Germantown, MD). A cDNA library of mRNA was constructed using SuperScript™ III Reverse Transcriptase Kit (Invitrogen, Waltham, MA). Samples were ran on an ABI One Step machine for the following markers: RPS6, RPS27, RPS29, RPL13, MALAT1, and B2M (30 (link), 31 (link)). deltaCT (dCT) was determined by CT (B2M) – CT (Target).
Hilliard K.A., Throm A.A., Pingel J.T., Saucier N., Zaher H.S, & French A.R. (2022). Expansion of a novel population of NK cells with low ribosome expression in juvenile dermatomyositis. Frontiers in Immunology, 13, 1007022.
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