Dopamine hydrochloride

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, Poland, China, United Kingdom

Dopamine hydrochloride is a chemical compound used in laboratory settings. It is a crystalline solid that is soluble in water. Dopamine hydrochloride is commonly used as a reference standard or analytical reagent in various scientific applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Penicillin, by Thermo Fisher Scientific (4 mentions) Streptomycin, by Thermo Fisher Scientific (4 mentions) Hoechst 33342, by Thermo Fisher Scientific (4 mentions) Silver nitrate agno3, by Thermo Fisher Scientific (3 mentions) Doxorubicin hydrochloride, by LC Laboratories (2 mentions) Bto nps, by Thermo Fisher Scientific (2 mentions) Vdf trfe, by Arkema (2 mentions) Formic acid, by Merck Group (2 mentions) Uric acid, by Thermo Fisher Scientific (2 mentions) Ammonium hydroxide solution, by Merck Group (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) Sodium bicarbonate, by Merck Group (2 mentions) Perfluoropentane pfp, by Strem Chemicals (2 mentions) Bovine serum albumin (bsa), by Merck Group (2 mentions) Chitosan, by Merck Group (2 mentions) Tryptic soy broth (tsb), by BD (2 mentions) Fibronectin, by Merck Group (1 mentions) Muse annexin 5 and dead cell assay, by Merck Group (1 mentions) Wst 1 cell proliferation reagent, by Takara Bio (1 mentions)

Close spelling variants of this product

Dopamine (28 citations) Dopamine hydrochloride 99%, (3 citations) Dopamine hydrochloride (DA-HCl; (2 citations) Dopamine hydrochloride (C8H11NO2•HCl, (2 citations) Dopamine hydrochloride salt (2 citations)

57 protocols using dopamine hydrochloride

Dendrimers for Doxorubicin Delivery

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Dopamine hydrochloride was purchased from Alfa Aesar (Gdansk, Poland). Doxorubicin hydrochloride (DOX·HCl) was purchased from LC Laboratories (Boston, MA, USA). PAMAM dendrimers G 3.0, phosphate buffer saline (PBS), Menadione, sodium tetraphenylborate, tris (hydroxymethyl) aminomethane, O-[N-(3-Maleimidopropionyl)aminoethyl]-O′-[3-(N-succinimidyloxy)-3-oxopropyl]heptacosaethylene glycol (molecular weight of 1570.76 g/mol), fetal bovine serum (FBS), penicillin, streptomycin, fibronectin, bovine collagen type I, bovine serum albumin, 0.1% poly-l-lysine, Muse^® Oxidative Stress Assay and MUSE^® Annexin V and Dead Cell assay were purchased from (Merck Darmstadt, Germany). Bronchial Epithelial Basal Medium (BEBM) and BEGM Bullet Kit were purchased from Lonza (Basel, Switzerland). WST-1 Cell Proliferation Reagent was purchased from Takara Bio (Shiga, Japan). Calcein AM, ethidium homodimer-1, Hoechst 33342, Minimum Essential Medium Eagle (MEM), dulbecco’s phosphate buffered saline (DPBS), 4% formaldehyde solution, sodium pyruvate, sodium pyruvate were purchased from Thermo Fisher Scientific (Waltham, MA, USA).

Grześkowiak B.F., Maziukiewicz D., Kozłowska A., Kertmen A., Coy E, & Mrówczyński R. (2021). Polyamidoamine Dendrimers Decorated Multifunctional Polydopamine Nanoparticles for Targeted Chemo- and Photothermal Therapy of Liver Cancer Model. International Journal of Molecular Sciences, 22(2), 738.

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Synthesis of Electrochemical Catalysts

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Cobalt (II) nitrate hexahydrate (98%), iron (II) sulfate heptahydrate (98%), zinc nitrate hexahydrate (99%), 2-methylimidazole (99%) zinc acetate dihydrate (98%), Pt/C (20%), dopamine hydrochloride (99%), 3-tris (hydorxymethyl) aminomethane (99.8%-100.1%), sodium thiocyanate (98%), potassium hydroxide (99.98%), sodium acetate trihydrate (99%), glacial acetic acid (99.9985%), and zinc foil (99.994%) were purchased from Alfa Aesar. Methanol and ethanol were received from Beijing Chemical Work Co. in analytic grade (AR). All chemicals were used as received without further purification. Nafion® solution (5 wt%, DuPont) was obtained from commercial suppliers. Milli-Q ultrapure water (resistance of 18.2 MΩ·cm at 25 °C) was used for all experiments.

Jiang Z., Liu X., Liu X.Z., Huang S., Liu Y., Yao Z.C., Zhang Y., Zhang Q.H., Gu L., Zheng L.R., Li L., Zhang J., Fan Y., Tang T., Zhuang Z, & Hu J.S. (2023). Interfacial assembly of binary atomic metal-Nx sites for high-performance energy devices. Nature Communications, 14, 1822.

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Synthesizing Dopamine-Functionalized PAN Membranes

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Polyacrylonitrile (PAN, Mw = 150,000), dimethylformamide (DMF), and tris (hydroxymethyl) aminomethane (>99%) were purchased from Sigma-Aldrich. Dopamine hydrochloride (99%) and silver nitrate (AgNO₃, 99%) were purchased from Alfa Aesar. Silica nanoparticles used for membrane rejection tests were obtained from XFNano (China). The manufacturer reports a mean particle diameter of 500 nm.

Wang J., Wu Y., Yang Z., Guo H., Cao B, & Tang C.Y. (2017). A novel gravity-driven nanofibrous membrane for point-of-use water disinfection: polydopamine-induced in situ silver incorporation. Scientific Reports, 7, 2334.

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Destabilized ATP Aptamer Purification

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The “destabilized” ATP aptamer (5′- CTGGGGGAGTATTGCGGAGGAAA-3′) oligonucleotide sequence^{24 (link)} was purified using dual HPLC (Biosearch Technologies, CA) and used as received. Tris-2-carboxyethyl-phosphine (TCEP), ferrocenemethanol (FcMeOH), 6-mercapto-1-hexanol (99%), Trizma (tris) base (2-amino- 2-(hydroxymethyl)-1,3-propanediol, magnesium chloride (MgCl₂), sulfuric acid (H₂SO₄), potassium chloride (KCl), hydrogen peroxide (H₂O₂), hydrochloric acid (HCl) sodium chloride (NaCl), adenosine triphosphate (ATP), dopamine hydrochloride (99%) (Alfa Aesar), dimethyl sulfoxide (DMSO), and 10× Tris-EDTA were used as received from Sigma- Aldrich. All solutions were prepared using autoclaved, ultrapure water (18.0 MΩ cm at 25°C) using a Biopak Polisher Millipore ultra purification system (Millipore, Billerica, MA).

Santos-Cancel M., Simpson L.W., Leach J.B, & White R.J. (2019). Direct, Real-Time Detection of Adenosine Triphosphate Release from Astrocytes in 3-Dimensional Culture Using an Integrated Electrochemical, Aptamer-Based Sensor. ACS chemical neuroscience, 10(4), 2070-2079.

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Chemical Reagents and Solvents Characterization

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All of the chemical reagents were purchased from Sigma-Aldrich (Poznan, Poland), except for the dopamine hydrochloride (Alfa Aesar, Gdansk, Poland) and doxorubicin hydrochloride (LC Laboratories, Boston, MA, USA). All reagents and solvents were of reagent-grade quality. For all experiments, Milli-Q deionized water (resistivity 18 MΩ·cm⁻¹) was used. 6-thio-β-cyclodextrin (SH-βCD) was kindly donated by CycloLab, (Budapest, Hungary).

Mrówczyński R., Jędrzak A., Szutkowski K., Grześkowiak B.F., Coy E., Markiewicz R., Jesionowski T, & Jurga S. (2018). Cyclodextrin-Based Magnetic Nanoparticles for Cancer Therapy. Nanomaterials, 8(3), 170.

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Fabrication of Polarized BaTiO3/P(VDF-TrFE) Nanocomposite Membranes

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The BaTiO₃/P(VDF-TrFE) nanocomposite membranes were fabricated based on our previously reported protocol [16 (link)]. Briefly, BTO NPs (99.9%, average particle size of 100 nm, Alfa Aesar) were dispersed ultrasonically in 0.01 mol/L of dopamine hydrochloride (99%, Alfa Aesar) aqueous solution, followed by stirring for more than 12 h at 60 °C to modify the nanoparticles. Subsequently, the modified BTO NPs together with the P(VDF-TrFE) co-polymer powders (70/30 mol% VDF/TrFE, Arkema) were both dissolved in N, N-dimethylformamide (DMF). After at least 3 rounds of repeated stirring and ultrasonication, the stable suspension was then cast into membranes of approximately 30 μm thickness and then dried at 55 °C for 10 h for solvent volatilization. For surface charge polarization, the membranes were annealed at 90 °C and 120 °C respectively for 30 min and were then treated by corona poling at room temperature for 30 min. Therefore, the membranes were differentially polarized with varying surface charges, with non-charged (NC, 55 °C, no poling), low-charged (LC, 55 °C, poling), mid-charged (MC, 90 °C annealing, poling) and high-charged (HC, 120 °C annealing, poling) nanocomposite membranes being obtained.

Li X., Heng B.C., Bai Y., Wang Q., Gao M., He Y., Zhang X., Deng X, & Zhang X. (2022). Electrical charge on ferroelectric nanocomposite membranes enhances SHED neural differentiation. Bioactive Materials, 20, 81-92.

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HPLC Analysis of Amino Acids and Amines

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HPLC-grade acetonitrile and methanol were from Merck. Hydrochloric acid (HCl), glycine and hydroxylamine (>97.5%) were purchased from Reakhim. DEEMM and L-ornithine monohydrochloride (≥99%) was obtained from Fluka. Boric acid (≥99%) was purchased from Hopkin & Williams. Amino acid commercial standards (L-alanine, L-arginine hydrochloride, L-asparagine, L-aspartic acid, L-glutamine, L-glutamic acid, L-glycine, L-histidine, L-isoleucine, L-leucine, L-lysine, L-methionine, L-phenylalanine, L-proline, L-serine, L-threonine, L-tryptophan, L-tyrosine and L-valine, ≥99.5%), γ-aminobutyric acid (≥99%), formic acid (≥95%), putrescine (≥99.5%), phenyethylamine (≥99.5%), tyramine hydrochloride (≥98%), ethylamine hydrochloride (98%), methylamine solution (40%), pyrrolidine (99%), ethanolamine (≥98%) and taurine (≥99%) were purchased from Sigma; dopamine hydrochloride (99%), histamine dihydrochloride (≥98%) were purchased from Alfa Aesar; L(+)-α-aminobutyric acid (98%) were purchased from Acros Organics; β-aminobutyric acid (98%) were purchased from BLD Pharmatec. Ultrapure water was supplied by a Millipore Milli-Q Advantage A10 (Millipore) and 0.75 M borate buffer at pH = 9 was prepared in deionized water.

Marengo A., Maciel L.S., Cagliero C., Rubiolo P, & Herodes K. (2023). Free Amino Acids and Biogenic Amines Profiling and Variation in Wild and Sub-Endemic Cardueae Species from Sardinia and Corse. Plants, 12(2), 319.

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Electrochemical Detection of Biomolecules

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All aqueous solutions were prepared using 18 MΩ·cm water
(NANOpure Diamond, Barnstead; Dubuque, IA, USA) and analytical grade reagents.
Copper (II) sulfate was purchased from Fisher Scientific (Fair Lawn, NJ, USA).
D-glucose was acquired from Aldrich Chemical Company, Inc. (Milwaukee, WI, USA).
Uric acid and dopamine hydrochloride were obtained from Alfa Aesar (Ward Hill,
MA, USA). Acetylsalicylic acid, diclofenac sodium salt, and L-ascorbic acid were
purchased from Sigma Aldrich (St. Louis, MO, USA). D-fructose was acquired from
Lancaster Synthesis, Inc. (Pelham, NH, USA). All reagents were used without
further treatment. Unless specifically stated, the electrochemical measurements
were performed in 100 mM NaOH solution (pH = 13.0). The D-glucose stock
solutions were prepared dissolving the corresponding amount of solid in 100 mM
NaOH solution and stored in the fridge after use. Standard solutions of
L-ascorbic acid (AA), Uric acid (UA), dopamine hydrochloride (DA), D-fructose,
and diclofenac sodium salt were freshly prepared also in 100 mM NaOH solution
before each experiment. Soft drinks used as real samples were obtained from a
local market.

Duran G.M., Benavidez T.E., Giuliani J.G., Rios A, & Garcia C.D. (2016). Synthesis of CuNP-Modified Carbon Electrodes Obtained by Pyrolysis of Paper. Sensors and actuators. B, Chemical, 227, 626-633.

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Hydrogel Synthesis and Characterization

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Acrylamide was purchased from MP Biomedicals LLC (France, purity ≥ 99%, Acrylamide acid <0.001%), lithium bromide (≥99%), sodium bromide (≥99%), sodium bicarbonate (≥99.5%), methacrylate anhydride (99%), N,N′-MethylenebisAcrylamide (MBAA, purity ≥ 99.5%), microcrystalline cellulose (MCC, size exclusion, ca 50 μm particle size), 2,2,6,6-tetramethylpiperidine 1-oxyl (TEMPO, 98%), phenylboronate Acrylamide (PBAAm, 98%), phosphotungstic acid hydrate (PTA, ≤ 0.002% total nitrogen), 2,4,6-trimethylbenzoyl chloride (97%) were bought from Sigam-Aldrich (Germany). Dimethoxyphenylphosphine (97%), dopamine hydrochloride (99%) and 2-butanone (99%) were received from Alfa Aesar (Germany). Sodium hydroxide (99%), borax-NaOH buffer (pH = 10.00, accuracy: ± 0.02 pH, reference temperature 20 °C) and sodium hypochlorite solution (14%, active chlorine) were purchased from TH Geyer (Germany). DI water (conductivity <1 μS/cm, room temperature) was used in all steps and all solvents were used directly from TH Geyer (Germany) without further treatment. Dopamine methylacrylate (DMA) and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) were prepared using the reported method^{54 (link),55 (link)}.

Xu D., Yang Y., Emmerich L., Wang Y, & Zhang K. (2023). Divergent Deborah number-dependent transition from homogeneity to heterogeneity. Nature Communications, 14, 6003.

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Dopamine-Induced Cell Viability Assay

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Dopamine hydrochloride, 99%, was purchased from Alfa Aesar. Dimethyl sulfoxide (DMSO) and 3-(4,5-dimethylthiazol-2yl)-2,5diphenyl tetrazolium bromide (MTT) were purchased from Sigma. Recombinant Mouse FGF basic and Mouse/Rat FGF basic Quantikine ELISA Kit were purchased from R&D Systems. BCA Protein Assay Kit was purchased from Abcam. All other commercially available solvents were obtained from Fisher Scientific Company.

Firoozi N, & Kang Y. (2020). Immobilization of FGF on Poly(xylitol dodecanedioic Acid) Polymer for Tissue Regeneration. Scientific Reports, 10, 10419.

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