Bis 2 ethylhexyl phthalate dehp

Both 1,2,4-benzenetricarboxylic acid 1,2-bis(2-ethylhexyl) ester and bis(2-ethylhexyl) phthalate (DEHP) were purchased from Toronto Research Chemicals (ON, Canada). N-hydroxysulfosuccinimide sodium salt (Sulfo-NHS) and N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC-HCl) were purchased from CovaChem (IL, USA). Magnetic beads (Dynabeads MyOne Streptavidin C1) were purchased from Thermo Fisher Scientific (CA, USA). Proprietary bis(2-ethylhexyl) phthalate (DEHP) aptamers were purchased from Base Pair Biotechnologies (TX, USA). A biotin and an 18-atom hexa-ethyleneglycol spacer were added at the 5’-end of the aptamer. Hydroxylamine hydrochloride, sodium hydroxide, silver nitrate, tetraethyl orthosilicate (TEOS), (3-aminopropyl) trimethoxysilane (APTMS), ammonium hydroxide (28%), 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB), sodium chloride (NaCl), sodium phosphate, ethanol, dimethyl sulfoxide (DMSO), and isopropanol were purchased from Sigma Aldrich (MO, USA). Milli-Q ultrapure water (18.2 MΩ cm⁻¹) was used in all the procedures. The mineral water and carbonated drinks in polyethylene terephthalate(PET) bottles were purchased from a local supermarket (TX, USA).