E el r0505c

Manufactured by Elabscience

Sourced in China

The E-EL-R0505c is a lab equipment product manufactured by Elabscience. It is a core component used in research and laboratory applications, but a detailed description cannot be provided while maintaining an unbiased and purely factual approach. Further information about the intended use or specific functionalities of this product is not available.

Automatically generated - may contain errors

Lab products found in correlation

E el r0012c, by Elabscience (3 mentions) E el r0019c, by Elabscience (2 mentions) E el r0015c, by Elabscience (2 mentions) Ab1872, by Abcam (1 mentions) Ab219800, by Abcam (1 mentions) Hemin, by Merck Group (1 mentions) Sc 374615, by Santa Cruz Biotechnology (1 mentions) Znppix, by Merck Group (1 mentions) Multiskan spectrum spectrophotometer, by Thermo Fisher Scientific (1 mentions) Corm 2, by Merck Group (1 mentions) E el r0567c, by Elabscience (1 mentions) Ab134047, by Abcam (1 mentions) E el h6005, by Elabscience (1 mentions) E el h0149c, by Elabscience (1 mentions) E el h1554c, by Elabscience (1 mentions) Elisa reader, by Bio-Rad (1 mentions) Seg886hu, by Cloud-Clone (1 mentions) Seg886ra, by Cloud-Clone (1 mentions) E el r2856c, by Elabscience (1 mentions) E el h0109c, by Elabscience (1 mentions)

6 protocols using e el r0505c

Hemin and CORM-2 Inflammasome Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hemin (no. 51280), zinc protoporphyrin IX (ZnPPIX) (no.282820), Carbon monoxide (CO)-releasing molecule 2 (CORM-2) (no.288144), and dextran 4000 Da (FD-4) (46944) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Dimethylsulfoxide (DMSO) (D3870), phosphate buffer saline (PBS) (P1020), and 4% paraformaldehyde (P1110) were obtained from Beijing Solarbio Science & Technology Co. Ltd. (Beijing, China). CORM-2 was dissolved in 0.5% of DMSO at 37°C in a sterile incubator for 24 h, and the resulting inactive CORM-2 (iCORM-2) was used as a negative control. Antibodies used were to caspase-1 (ab1872; Abcam, Shanghai, China), gasdermin D (GSDMD) (ab219800; Abcam, Shanghai, China), and caspase-11 p20 (sc-374615; Santa Cruz Biotechnology, Texas, USA). Enzyme-linked immunosorbent assay (ELISA) kits included rat tumor necrosis factor-α (TNF-α) (E-EL-R0019c), rat interleukin-1β (IL-1β) (E-EL-R0012c), rat IL-18 (E-EL-R0567c), and rat high mobility group box protein 1 (HMGB1) (E-EL-R0505c) (Elabscience Biotechnology Co., Ltd., Wuhan, China). The ELISA results were analyzed using a Multiskan Spectrum spectrophotometer (Thermofisher Scientific, Waltham, MA, USA).

Wang H., Zhang S., Zhao H., Qin H., Zhang J., Dong J., Zhang H., Liu X., Zhao Z., Zhao Y., Shao M., Wu F, & Zhang W. (2020). Carbon Monoxide Inhibits the Expression of Proteins Associated with Intestinal Mucosal Pyroptosis in a Rat Model of Sepsis Induced by Cecal Ligation and Puncture. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e920668-1-e920668-14.

+ Open protocol

+ Expand

Rat Inflammation Biomarker ELISA Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols

Rat IL-1β (EK301BHS), TNF-α (EK382HS), and IL-6 (EK306/3) enzyme-linked immunosorbent assay (ELISA) kits were purchased from Multisciences Biotech (Hangzhou, Zhejiang, China). Rat HMGB1 (E-EL-R0505c), thromboxane B2 (TXB2, E-EL-R0965c), 6-keto prostaglandin F1 alpha (6-keto-PGF1α, E-EL-0054c) ELISA kits were procured commercially (Elabscience Biotechnology, Wuhan, Hubei, China). Rabbit polyclonal antibodies against HMGB1 (#6893S), receptor for advanced glycation end products (RAGE, #6996S), p-NF-κB p65 (#3033S), NF-κB p65 (#8242S), Janus kinase (JNK; #9252s), p-JNK (#4668s), ERK (#9102S), p-ERK (#4376s), p38 MAPK (#8690S), p-p38 MAPK (#4511S), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; #5174S) were purchased from Cell Signaling Technology (Beverly, MA, United States). Rabbit monoclonal antibodies against VCAM-1 (#ab134047) and mouse monoclonal antibodies against ICAM-1 (#ab171123) were purchased from Abcam (Cambridge, MA, United States).

Zou C., Liu L., Huang C, & Hu S. (2022). Baiying qingmai formulation ameliorates thromboangiitis obliterans by inhibiting HMGB1/RAGE/NF-κB signaling pathways. Frontiers in Pharmacology, 13, 1018438.

+ Open protocol

+ Expand

Quantification of Inflammatory Mediators in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

Twenty-four hours after the last administration, 2 mL of venous blood was collected from each experimental rat by subclavian venipuncture. The concentrations of TgAbs, HMGB1, TNF-α, IL-6, IL-1β and monocyte chemoattractant protein-1 (MCP-1) in rats were detected with ELISA kits according to the manufacturer’s protocol (TgAbs: #m1003044, Kangting, Tianjin, China; HMGB1: E-EL-R0505c, Elabscience, China; TNF-α: EK382/3-96, Multisciences, China; IL-6: ER306/3-96, China; IL-1β: E-EL-R0012c, Elabscience, China; MCP-1: E-EL-R0633c, Elabscience, China). The secretion of HMGB1, TNF-α, IL-6, IL-1β and MCP-1 by cell lines was detected with ELISA kits according to the manufacturer’s protocol (HMGB1: E-EL-H1554c, Elabscience, China; TNF-α, EK182HS-48, Multisciences, China; IL-6, EK106/2-48, Multisciences, China; IL-1β: E-EL-H0149c, Elabscience, China; MCP-1: E-EL-H6005, Elabscience, China).

Guo Q., Qu H., Zhang H, & Zhong X. (2021). Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling. Drug Design, Development and Therapy, 15, 4559-4574.

+ Open protocol

+ Expand

Assessing Inflammatory Markers in Rat Model

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood was collected at 4 weeks after treatment with sivelestat sodium hydrate while rats were under anesthesia (35 mg/kg pentobarbital) and centrifuged at 12,000 × g for 10 min at 4°C. The supernatant was collected and used to determine the levels of TNF-α (EM010-96) and IL-6 (EM004-96) production and using commercial enzyme-linked immunosorbent assay (ELISA; ExCell Bio, Taichang, China) kits and an ELISA reader (Bio-Rad Laboratories, Inc.) at 405 nm. Nitrite concentrations were measured using a commercial kit (A038; Nanjing Jiancheng Biology Engineering Institute, Nanjing, China) and an ELISA reader (Bio-Rad Laboratories, Inc.) at 540 nm. HMGB1 secretion were measured using a commercial kit (E-EL-R0505c; Elabscience Biotechnology Co., Ltd., Wuhan, China) at 450 nM.

Yu X., Zhao L., Yu Z., Yu C., Bi J., Sun B, & Cong H. (2017). Sivelestat sodium hydrate improves post-traumatic knee osteoarthritis through nuclear factor-κB in a rat model. Experimental and Therapeutic Medicine, 14(2), 1531-1537.

+ Open protocol

+ Expand

Multiplex Protein Detection in Biological Fluids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cold‐inducible RNA‐binding protein (SEG886Hu and SEG886Ra, Cloud‐Clone Corp.), mitochondrial transcription factor A (TFAM; SEH050Hu, Cloud‐Clone Corp.), interleukin 6 (IL‐6; E‐EL‐H0109c and E‐EL‐R0015c, Elabscience), interleukin 1 beta (IL‐1β; E‐EL‐R0012c, Elabscience), tumour necrosis factor‐alpha (TNF‐α; E‐EL‐R2856c, Elabscience) and high mobility group protein B1 (HMGB1; E‐EL‐R0505c, Elabscience) ELISA kits were used to detect these molecules in the UW solution, human serum and rat serum.

Liu W., Fan Y., Ding H., Han D., Yan Y., Wu R., Lv Y, & Zheng X. (2021). Normothermic machine perfusion attenuates hepatic ischaemia‐reperfusion injury by inhibiting CIRP‐mediated oxidative stress and mitochondrial fission. Journal of Cellular and Molecular Medicine, 25(24), 11310-11321.

+ Open protocol

+ Expand

Blood Sampling and ELISA Analysis in Rat Model

Cited 3 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

On the 3rd day, after HRV monitoring, 6 rats from each group were anesthetized with isoflurane inhalation [17 (link), 18 (link)]; then, the chest cavity was dissected, and 2 mL of blood was taken from the right atrium for ELISA and 2 mL for flow cytometry (FCM). For ELISA, blood was preserved at room temperature for 2 h prior to centrifugation at 3000 r/min at 4°C for 10 min; then, the upper layer of the serum was separated and stored in the refrigerator at -80°C for detection. For FCM, fresh blood was taken for testing. According to the manufacturer's instructions [19 (link)], the serum levels of TNF-α, IL-1α, IL-10, IL-6, HMGB1, and sCD14 were determined with ELISA.
The main related antibodies include: rat anti-TNF-α (lot number: E-EL-R0019c, manufacturer: Elabscience), rat anti-IL-1α (lot number: E-EL-R0011c, manufacturer: Elabscience), rat anti-IL-6 (lot number: E-EL-R0015c, manufacturer: Elabscience), rat anti-IL-10 (lot number: E-EL-R0016c, manufacturer: Elabscience), rat anti-HMGB-1 (lot number: E-EL-R0505c, manufacturer: Elabscience), and rat anti-sCD14 (lot number: CSB-E11178r, manufacturer: Cusabio).

Li H.B., Zhou Y., Zhao A.H, & Guo L.L. (2020). Exploring the Mechanism on the Medullary Visceral Zone Inhibiting the Cholinergic Anti-inflammatory Pathway Induced by Sepsis. Mediators of Inflammation, 2020, 1320278.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!