Middlebrook 7h10 mb 7h10 agar

Isolates of Mycobacterium abscessus, Mycobacterium chelonae, Mycobacterium gordonae, and Mycobacterium peregrinum maintained in our culture collection were used. All were isolated from zebrafish facilities in the United States and were previously identified based on hsp65 gene sequencing as previously described (Kent et al. 2004 ; Poort et al. 2006 (link); Whipps et al. 2007a (link); Whipps et al. 2007b (link)). All isolates were grown at 28-30°C for seven days on solid-phase Middlebrook 7H10 (MB 7H10) agar (BD Biosciences 262710) supplemented with oleic albumin dextrose catalase (OADC, BD Biosciences 211886), prior to preparation for disinfection treatments.

Isolates maintained in our culture collection are described in Table 1. All organisms have previously been identified based on hsp65 gene sequencing as described previously (Kent et al. 2004 (link); Poort et al. 2006 (link); Whipps et al. 2007a (link); Whipps et al. 2007b (link)). In addition to these isolates, reference cultures of rapidly growing Mycobacterium salmoniphilum (ATCC13758) and slow growing M. marinum (ATCC927) are also included in this study. All isolates were grown on solid-phase Middlebrook 7H10 (MB 7H10) agar (BD Biosciences 262710) supplemented with oleic albumin dextrose catalase (OADC, BD Biosciences 211886) at 28-30°C for seven (rapid-growing) or 14 (slow-growing) days prior to MIC testing.