Each of the OMP preparations (5 μg) were run on the Novex Bis-Tris pre-cast gel system with MOPS running buffer according to the manufacturer's instructions (Life Technologies). Ammoniacal silver staining was carried out to visualize proteins. Western blotting was carried out using nitro-cellulose membranes (Bio-Rad) and standard protocols68 . All mouse (AD6 anti-HMW1/2A34 (link); 1F4 anti-Hia35 (link)) and rabbit (LB1 anti-OMP P5 (ref. 28 (link)); chimV4 anti-OMP P5; anti-OMP P5) primary antibodies were used at a dilution of 1:2,500; all chinchilla (anti-OMP P2; anti-LPD27 (link); anti-PDM27 (link); anti-OMP P5/P6) primary antibodies at a dilution of 1:250. Anti-mouse-AP and anti-rabbit-AP secondary antibodies were used at a dilution of 1:5,000 (Sigma-Aldrich); protein A-AP secondary antibody was used at a dilution of 1:500 (Sigma-Aldrich) in blots where chinchilla primary antibodies were used. Blots were developed using SigmaFAST NBT/BCIP tablets according to the manufacturer's instructions (Sigma-Aldrich). All the primary antibodies were raised by the authors laboratories, with specific references for those described previously.
Anti mouse ap and anti rabbit ap secondary antibodies
Manufactured by Merck Group
Anti-mouse-AP and anti-rabbit-AP secondary antibodies are laboratory reagents used in immunoassays and other analytical techniques. They are conjugated with the enzyme alkaline phosphatase (AP), which allows for detection and quantification of target proteins or antigens in samples. These secondary antibodies bind to primary antibodies raised against mouse or rabbit proteins, respectively, enabling signal amplification and visualization of the analyte of interest.
Automatically generated - may contain errors
Lab products found in correlation
Mops running buffer, by Thermo Fisher Scientific (2 mentions)
Novex bis tris pre cast gel system, by Thermo Fisher Scientific (2 mentions)
Protein a ap secondary antibody, by Merck Group (2 mentions)
Sigmafast nbt bcip tablets, by Merck Group (2 mentions)
Nitrocellulose membrane, by Bio-Rad (2 mentions)
2 protocols using anti mouse ap and anti rabbit ap secondary antibodies
1
Protein Expression Analysis by Western Blot
2
Protein Visualization and Immunoblotting
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Each of the OMP preparations (5 μg) were run on the Novex Bis-Tris pre-cast gel system with MOPS running buffer according to the manufacturer's instructions (Life Technologies). Ammoniacal silver staining was carried out to visualize proteins. Western blotting was carried out using nitrocellulose membranes (Bio-Rad) and standard protocols68 . All mouse (AD6 anti-HMW1/2A34 (link); 1F4 anti-Hia35 (link)) and rabbit (LB1 anti-OMP P5 (ref. 28 (link)); chimV4 anti-OMP P5; anti-OMP P5) primary antibodies were used at a dilution of 1:2,500; all chinchilla (anti-OMP P2; anti-LPD27 (link); anti-PDM27 (link); anti-OMP P5/P6) primary antibodies at a dilution of 1:250. Anti-mouse-AP and anti-rabbit-AP secondary antibodies were used at a dilution of 1:5,000 (Sigma-Aldrich); protein A-AP secondary antibody was used at a dilution of 1:500 (Sigma-Aldrich) in blots where chinchilla primary antibodies were used. Blots were developed using SigmaFAST NBT/BCIP tablets according to the manufacturer's instructions (Sigma-Aldrich). All the primary antibodies were raised by the authors laboratories, with specific references for those described previously.
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