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Mouse anti cdk2

Manufactured by Cell Signaling Technology
Sourced in United States

Mouse anti-CDK2 is a primary antibody that recognizes the cyclin-dependent kinase 2 (CDK2) protein. CDK2 is a key regulator of cell cycle progression, playing a critical role in the G1/S and G2/M transitions. This antibody can be used in various immunological techniques to detect and study the expression and localization of CDK2 in biological samples.

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2 protocols using mouse anti cdk2

1

Analyzing Cell Cycle Regulators

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Western blots were performed using Mouse anti-CDK2, anti-CDK4, anti-CDK6, and anti-cyclin D1 were purchased as part of the Cell Cycle Regulation Sampler Kit (Cell Signaling Technology, Danvers, MS, USA). Mouse anti-β-actin was purchased from Sigma-Aldrich (St. Louis, MO, USA). Protein detection was performed with Super Signal chemiluminescence substrate (Pierce, Rockford, IL, USA).
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2

Biochanin A Signaling Pathway Protocol

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Biochanin A was purchased from Jinhui Biology, dissolved in DMSO as a stock concentration of 100 mmol/L, and stored in the dark at −20°C. Figure 1 shows the chemical structural of Biochanin A.
The different concentrations of Biochanin A used for different experiments were diluted with high-glucose DMEM. The antibodies used for Western blotting were as follows: rabbit anti-cyclin A, anti-P21, anti-Caspase-3, anti-Bcl-2, anti-Bax, and mouse anti-CDK2, anti-β-actin (Cell Signaling Technology, CST)
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