Reagents: Imiquimod (3M Pharmaceuticals R-837) was dissolved in sterile acidic deionized distilled water (pH 2.5) at a concentration of 1 mg/ml [18] , sterilized by 0.22 µm membrane filtration and diluted at working concentration in PBS. Corresponding control was identically prepared with the same volume of acidic deionized distilled water (pH 2.5) and PBS, but without Imiquimod. The final pH value of Imiquimod solution and control solutions used to inject the mice was always adjusted between 6.5 and 7.
Imiquimod
Manufactured by 3M
Sourced in United States, United Kingdom
Imiquimod is a laboratory product manufactured by 3M. It is a synthetic compound that can be used in various research and development applications. The core function of Imiquimod is to act as a toll-like receptor agonist, which can stimulate the immune system. Further details on the intended use of this product are not provided.
Automatically generated - may contain errors
Lab products found in correlation
Aldara cream, by 3M (2 mentions)
C57bl 6j mice, by Japan SLC (1 mentions)
Aldara, by 3M (1 mentions)
Km bmb1 25, by Mitutoyo (1 mentions)
Sb225002, by Merck Group (1 mentions)
Tnf α, by MedChemExpress (1 mentions)
Nanozoomer digital pathology ndp view2, by Hamamatsu Photonics (1 mentions)
Trucount tube, by BD (1 mentions)
Liberase, by Roche (1 mentions)
Montanide isa 51 adjuvant, by Seppic (1 mentions)
4 phenylbutyrate 4 pba, by Merck Group (1 mentions)
Powerplex 18d system, by Promega (1 mentions)
13 protocols using imiquimod
1
Imiquimod Solution Preparation Protocol
2
Topical Imiquimod-Induced Psoriasis Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female six-week-old C57BL/6J mice were purchased (Japan SLC Inc., Shizuoka, Japan) and maintained under conventional conditions. Psoriasis-like skin inflammation was induced on the shaved back skin of C57BL/6J mice by the topical application of Aldara cream containing 5% imiquimod (3M Pharmaceuticals, Northridge, CA, USA). The topical application was repeated daily for a week until the appearance of psoriasis-like skin inflammation. To examine the therapeutic effect of recombinant ERDR1 (rERDR1) on psoriasis-like skin inflammation, rERDR1 was injected intraperitoneally once a day for 7 consecutive days. Phosphate buffered saline (PBS) was injected as a vehicle control, while 10 or 100 µg/kg rERDR1 was injected to assess the effects of rERDR1 on the psoriasis-like skin inflammation. Each group was composed of five mice. The experiments were performed as three independent experiments. All experimental procedures with mice were approved by the Institutional Animal Care and Use Committee of Sookmyung Women’s University (Permission date: 18 June 2013; SM-IACUC-2013-0401-002).
3
SHP2 Deletion in Neutrophils
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All the experimental animals were purchased from GemPharmatech Co. Ltd., and animal were maintained in specific pathogen-free conditions at GemPharmatech Co.
Ltd and Experimental Animal Center at Nanjing University. Shp2 flox/flox mice were crossed with S100a8-cre mice on the C57BL/6 background for more than two generations to generate Shp2 flox/flox S100a8-cre mice which SHP2 was conditional knocked out in neutrophils. we also constructed Padi4 knockout on the C57BL/6 background.
Mice were shaved and treated with imiquimod (H20160079, 3M Pharmaceuticals, USA) or petroleum successively. For different experimental needs, 10 mg/kg SHP099, or physiological saline solution was injected intraperitoneally (i.p.). Serum was isolated from the blood of mice which anesthetized with 0.05 mg/kg pelltobarbitalum natricum, and followed by retro-orbital sinus taken. Skin and serum were stored at -80 for future use. All the procedures were carried out in accordance with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health, USA) and ethical regulations of Nanjing university to guarantee animal welfare.
Ltd and Experimental Animal Center at Nanjing University. Shp2 flox/flox mice were crossed with S100a8-cre mice on the C57BL/6 background for more than two generations to generate Shp2 flox/flox S100a8-cre mice which SHP2 was conditional knocked out in neutrophils. we also constructed Padi4 knockout on the C57BL/6 background.
Mice were shaved and treated with imiquimod (H20160079, 3M Pharmaceuticals, USA) or petroleum successively. For different experimental needs, 10 mg/kg SHP099, or physiological saline solution was injected intraperitoneally (i.p.). Serum was isolated from the blood of mice which anesthetized with 0.05 mg/kg pelltobarbitalum natricum, and followed by retro-orbital sinus taken. Skin and serum were stored at -80 for future use. All the procedures were carried out in accordance with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health, USA) and ethical regulations of Nanjing university to guarantee animal welfare.
4
Aldara-Induced Skin Inflammation in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vivo treatments Mice were anesthetized with isoflurane and Aldara treatment was performed by applying daily 8 mg Aldara cream (5% Imiquimod; 3M Pharmaceuticals) on each mouse ear for the duration indicated in the text. DTx (Calbiochem, 500ng per mouse in PBS) was injected intraperitoneally (i.p.) on day 4 and 6 or 10 and 12 after starting the Aldara treatment. Anti-GM-CSF treatment was performed every second day by i.p. injection of 300 mg rat anti-mouse GM-CSF monoclonal Ab.
5
Imiquimod-Induced Psoriasis Mouse Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mouse models of psoriasis were developed in BALB/c mice by 3.125 mg of imiquimod (IMQ; 5% Aldara, 3M Pharmaceuticals, Los Angeles, CA, USA) in 62.5 mg lotion/day per 5 cm2 for six consecutive days. The IMQ-induced mouse models were intra-cutaneously injected with sh-EZH2/sh-NC lentivirus (1 × 109 pfu/100 μL) (GenePharma) for 3 days with the skin samples collected and stored at −80°C. Finally, the clinical score was evaluated by three independent researchers while the scores relating to the degree of erythema, desquamation, and thickness were rated from 0 to 4 (0, none; 1, mild; 2, moderate; 3, severe; 4, very severe).
6
Imiquimod-Induced Psoriasis-Like Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C57BL/6-TG2KO and C57BL/6-TG3KO mice were generated in our laboratory [21 (link)]. All mice used in this study were bred in the animal facility of the University of Roma “Tor Vergata” under specific pathogen-free conditions. All experiments were approved by the Institutional Animal Care and Use Committee (IACUC) and were carried out according to the Italian and European rules (D.L.116/92; C.E. 609/86; European Directive 2010/63/EU). For mice experiments licence n° 817/2016PR (Italian Ministry of Health). Male mice at 8 weeks of age were shaved using an electric shaver on the back skin and received topical applications of imiquimod (3.125 mg) from a commercially available cream (5%) daily for 10 days (Aldara; 3M Pharmaceuticals, St. Paul, MN, USA).
7
Imiquimod-Induced Psoriasis-like Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Aldara cream containing 5% imiquimod (3 M Health Care Limited, NDC 0089-0610-12, UK) was applied daily on the inner side of the mouse ears for 8 days to induce psoriasis-like inflammation [41 (link)]. Ear thickness was measured using a digital micrometer (#KM-BMB1-25; Mitutoyo, Kawasaki, Japan) every other day, and ear swelling was calculated as the difference between the thickness on the first day and the thickness on the measured day. To block the IL-1 receptor, an anti-IL-1R1 antibody was intraperitoneally injected into the mice (Clone: JAMA-147, #BE052, RRID: AB_2661843; Bioxcell, Lebanon, NH) every other day for systemic neutralization (12 mg/kg) and intranasally injected on alternate days for local neutralization (2 mg/kg), all 1 h before Aldara cream application. Control mice were treated with DPBS instead of the antibody. In another experiment, saccharin suspended in DPBS (8 mg/kg, 200 µg/200 µL onto each ear) or SB225002 (Sigma; 1 mg/kg, 10 µg/100 µL onto each ear) was administered onto the ear of mice by plastic bandage daily 1 h before Aldara cream application during the experimental period.
8
Evaluating Immunomodulatory Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Imiquimod (GTH110C, 3 M Health Care Limited, UK) was purchased from Jiangsu Provincial Hospital of Traditional Chinese Medicine. Shi-Bi-Man (SBM) was purchased from Sipimo Biotechnology Co., Ltd (Shenzhen, China). TSG was purchased from Chengdu Purifa Technology Development Co., Ltd. TNF-α was purchased from MedChemExpress USA. Benvitimod was purchased from guanhaobio (Guangdong, China).
9
Topical Delivery of UCB-MNC-sEV for Skin Inflammation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Animal experiments were approved by the ethical committee of the Spanish National Cardiovascular Research Center, and performed according to national and European regulations, respecting animal welfare guidelines and the 3R’s rule. Eight- to twelve-week-old C57BL/6 mice received daily applications of imiquimod (3M Pharmaceuticals, Saint Paul, MN, USA) on their shaved backs, for 6 consecutive days. One hour after every imiquimod application, 3 × 109 particles/cm2 UCB-MNC-sEV dissolved in hydrogel were delivered topically. Epidermal thickness was measured on H&E-stained samples, using the software NanoZoomer Digital Pathology NDP.view2 (Hamamatsu Photonics, Hamamatsu, Japan), from stratum basale to stratum granulosum, averaging 5 measurements per section, for a total of 20 data points per animal. For flow cytometry, skin was digested with 0.25 mg/mL Liberase (Roche, Basel, Switzerland) in serum-free RPMI, for 60 min at 37 °C. Skin cell suspensions were stained with fluorescently labelled antibodies, following Fc Block (Table 2 ). Absolute cell counts were performed using Trucount tubes (BD Biosciences, San Jose, CA, USA). RNA analyses were performed as described above.
10
Adjuvant-assisted Immunotherapy Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GM-CSF (Leukine) was manufactured by Berlex (Seattle, Washington, USA) as a clinical grade reagent, and was purchased from the UVA hospital pharmacy. Montanide ISA-51 adjuvant was purchased from Seppic (Paris, France) in sealed glass vials (3 mL/vial). Montanide ISA-51 had been prepared with oleic acid from a bovine source (‘animal’ formulation) for 23 participants and from a vegetable source for 5 participants.37 (link) DMSO (Cryoserv) was purchased from the manufacturer (Edwards Lifesciences Research Medical.; Irvine, California, USA). Imiquimod (Aldara; 5% cream, 0.25 g/packet) was manufactured by 3M (St. Paul, Minnesota, USA) as a clinical grade reagent and was purchased from the UVA hospital pharmacy.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!