Serum bone metabolism markers and sex hormones, including total 25-hydroxyvitamin D (25OHD), intact parathyroid hormone (PTH), Beta-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX), osteocalcin (OC), total testosterone, estradiol, luteinizing hormone (LH), follicle stimulating hormone (FSH), and sex hormone-binding globulin (SHBG) were measured using an automated Roche electrochemiluminescence system (Roche Diagnostic GmbH), following both the manufacturer's protocol and specialized assay laboratory quality control procedures. Early morning urine samples were collected at the patients' first visit. Urinary levels of PGE2 and PGE-M were detected using competitive enzyme-linked immunosorbent assays (ELISA; Cayman Chemical, Ann Arbor, MI, USA), which was described in our previous study 12 . The values were normalized to creatinine according to the manufacturer's instructions (Item 500141 for PGE2, Item 514531 for PGE-M, Item 500701 for creatinine; Cayman Chemicals, Ann Arbor, MI, USA).
Electrochemiluminescence system
Manufactured by Roche
Sourced in Germany, Switzerland
The Roche electrochemiluminescence system is a laboratory instrument designed for sensitive and accurate detection and quantification of various analytes in biological samples. The system utilizes electrochemical principles to generate luminescent signals, which are then measured and interpreted to provide analytical results.
Automatically generated - may contain errors
Lab products found in correlation
7600 020 automatic biochemistry analyzer, by Hitachi (3 mentions)
Elisa, by Cayman Chemical (1 mentions)
Standard kit, by Cayman Chemical (1 mentions)
Lunar prodigy dual energy x ray absorptiometry densitometer, by GE Healthcare (1 mentions)
Csb e12776r, by Cusabio (1 mentions)
Cobas integra 400 plus, by Roche (1 mentions)
Beckman cx 7 biochemical autoanalyzer, by Beckman Coulter (1 mentions)
Tartrate resistant acid phosphatase assay kit, by Beyotime (1 mentions)
1 25 oh 2d 125i ria kit, by DiaSorin (1 mentions)
7600 series automatic biochemical analyzer, by Hitachi (1 mentions)
19 protocols using electrochemiluminescence system
1
Serum and Urinary Markers of Bone Metabolism
2
Bone Turnover Biomarkers in Primary Hyperostosis
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Blood samples and 24 h urine were drawn after an overnight fast for biochemistry measurements. The Roche electrochemiluminescence system measured beta-C-telopeptides of type I collagen (β-CTX) (E170, Roche Diagnostics, Basel, Switzerland). Serum-free soluble receptor activator of nuclear factor-kappaB ligand (sRANK), osteoprotegerin (OPG), and DKK1 were measured by an enzyme-linked immunosorbent assay (ELISA) using standard kits (Human sRANKL (Total), Human osteoprotegerin, Biovendor, Brno, Czech Republic; Human DKK-1 Quantikine, R&D Systems). Urinary PGE2 and PGEM levels were measured by competitive ELISA using standard kits (Cayman Chemicals, Ann Arbor, Michigan, USA). All biochemical parameters were evaluated in PHO patients. Simultaneously, DKK1, sRANKL, and OPG were assessed in HCs.
3
Clinical and Genetic Evaluation of Skeletal Disorders
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Anthropology information (height, weight, age, etc.), comprehensive clinical evaluation, and family history were obtained. Pedigrees of the three families are shown in Figure 1 .
Serum total alkaline phosphatase (ALP), phosphorus, calcium, erythrocyte sedimentation rate (ESR), high‐sensitivity C‐reactive protein (hsCRP), and other biochemical indices were assessed by a Hitachi 7600–020 automatic biochemistry analyzer (HITACHI, Japan). Serum intact parathyroid hormone (iPTH), 25‐hydroxyvitamin D (25OHD), β‐CrossLaps of type 1 collagen‐containing cross‐linked C‐telopeptide (β‐CTX), and osteocalcin (OC) were measured by an automated Roche electrochemiluminescence system (Roche Diagnostic GmbH, Germany).
Bone scintigraphy with 99mTc‐methylene diphosphonate and radiological examinations were performed. Bone mass density (BMD, g/cm2) of the lumbar spine 1–4 (L1–4), left femoral neck and total hip were measured by a lunar prodigy dual‐energy X‐ray absorptiometry densitometer (GE Healthcare, Madison, USA).
Serum total alkaline phosphatase (ALP), phosphorus, calcium, erythrocyte sedimentation rate (ESR), high‐sensitivity C‐reactive protein (hsCRP), and other biochemical indices were assessed by a Hitachi 7600–020 automatic biochemistry analyzer (HITACHI, Japan). Serum intact parathyroid hormone (iPTH), 25‐hydroxyvitamin D (25OHD), β‐CrossLaps of type 1 collagen‐containing cross‐linked C‐telopeptide (β‐CTX), and osteocalcin (OC) were measured by an automated Roche electrochemiluminescence system (Roche Diagnostic GmbH, Germany).
Bone scintigraphy with 99mTc‐methylene diphosphonate and radiological examinations were performed. Bone mass density (BMD, g/cm2) of the lumbar spine 1–4 (L1–4), left femoral neck and total hip were measured by a lunar prodigy dual‐energy X‐ray absorptiometry densitometer (GE Healthcare, Madison, USA).
4
Serum Biomarkers of Bone Metabolism
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Serum testosterone level was measured with an automated Roche electrochemiluminescence system (Roche Diagnostics, Switzerland). Serum levels of calcium (Ca), phosphorus (P), total alkaline phosphatase (ALP, a marker of bone formation), and creatinine (Cr) were measured using an automatic biochemistry analyzer (Cobas Integra 400 plus, Roche kit) [22 (link)]. A commercial ELISA kit (Cat. No. CSB-E12776r, Cusabio, Wuhan, China) was used to detect the levels of C-telopeptide of type I collagen (CTX-I, a bone resorption marker) according to the manufacturer’s instructions, with intra-assay and inter-assay CVs both less than 15%.
5
Assessing Bone Metabolism and RAS Markers
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Blood samples were harvested at the end of the 12-week study period, centrifuged for 15 min at 1,000 × g at 2–8°C and stored at −70°C. Parameters for the test included serum calcium, inorganic phosphorous, and alkaline phosphatase, which were assessed by standard laboratory techniques (Roche Diagnostics GmbH, Germany). Bone turnover markers 1,25(OH)2D3, β-Crosslaps (β-CTX) and total N-terminal propeptide of type I procollagen (T-PINP) were measured using Cobas E411C kits (Roche Diagnostics GmbH) at the same central biochemical laboratory using an automated Roche electrochemiluminescence system (Roche Diagnostics GmbH). The main RAS components (ACE and Ang II) were measured using kits (Abcam, Cambridge, UK; catalog nos. ab155452 and ab47831). Intra-assay and inter-assay CVs of the biochemical parameters, bone turnover markers and main RAS components were <3%.
6
Metabolic and Bone Markers Assessment
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Blood samples were collected following overnight fasting. Triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were determined by colorimetry. Fasting blood glucose (FBG) and glycated hemoglobin (HbA1c) were measured by using the hexokinase method and high-performance liquid chromatography respectively. N-terminal mid-fragment of osteocalcin (N-MID), the most stable form of osteocalcin in serum, and C-peptide were measured by an automated Roche electrochemiluminescence system. The TyG index was determined as ln(fasting TG [mg/dL] × FBG [mg/dL]/2). HOMA-IR was computed with FBG and C-peptide levels using the HOMA calculator v2.2.3 (https://www.dtu.ox.ac.uk/HOMACalculator/ ).
7
Comprehensive Profiling of Paget's Disease
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Clinical data were collected, including sex, onset age, diagnosis age, detailed medical history data (including medication history, previous visiting information and family history). The clinical manifestations (bone pain, bone deformity and local swelling, etc.) and related complications (osteoarthritis, pathological fracture, hearing loss, headache, vision impairment, etc.) of PDB were recorded. In addition, X-ray images and whole bone scintigraphy with 99mTc-methylene diphosphonate (99mTc-MDP) was performed to determine the severity and the involved bone sites.
Serum ALP, phosphorus, calcium, and other biochemical indices were assessed by a Hitachi 7,600-020 automatic biochemistry analyzer (HITACHI, Japan). Serum intact parathyroid hormone (iPTH), 25-hydroxy-vitamin D (25OHD), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX) and osteocalcin (OC) were measured by an automated Roche electrochemiluminescence system (Roche Diagnostic GmbH, Germany).
Serum ALP, phosphorus, calcium, and other biochemical indices were assessed by a Hitachi 7,600-020 automatic biochemistry analyzer (HITACHI, Japan). Serum intact parathyroid hormone (iPTH), 25-hydroxy-vitamin D (25OHD), β-CrossLaps of type 1 collagen containing cross-linked C-telopeptide (β-CTX) and osteocalcin (OC) were measured by an automated Roche electrochemiluminescence system (Roche Diagnostic GmbH, Germany).
8
Biochemical Indicators in Serum
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Biochemical indicators included serum phosphate, total calcium, total alkaline phosphatase (ALP), intact parathyroid hormone (iPTH), 25-hydroxyvitamin D (25OHD), β-isomerized C-terminal telopeptide of type 1 collagen (β-CTX), and serum osteocalcin (OC). Biochemical indexes and BTMs were measured by a Hitachi 7600-020 automatic biochemistry analyzer and an automated Roche electrochemiluminescence system, respectively. Blood samples of 29 patients were drawn in the morning after fasting overnight.
Serum iFGF23 levels were measured by using a two-site ELISA kit (KAINOS Laboratories, Inc., Tokyo, Japan) with a detectable concentration range from 3 to 800 pg/ml. The reference range was 16.1–42.2 pg/ml (22 (link)).
Serum iFGF23 levels were measured by using a two-site ELISA kit (KAINOS Laboratories, Inc., Tokyo, Japan) with a detectable concentration range from 3 to 800 pg/ml. The reference range was 16.1–42.2 pg/ml (22 (link)).
9
Comprehensive Metabolic Profiling in Clinical Trials
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Biochemical variables were measured at each visit. A 12-h overnight fasting blood sample was collected for all participants to determine the bone metabolism, lipid profile, fasting plasma glucose (FPG), fasting insulin, glycated hemoglobin (HbA1c), total serum protein (TSP) and serum albumin (SA). Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) was assessed as fasting insulin (mIU/L) × fasting glucose (mM)/22.5. HOMA-β was calculated as follows: (20 × insulin [mIU/mL])/(glucose [mmol/L]– 3.5), as previously described (23 (link)). Bone metabolism parameters, including parathyroid hormone (PTH), 25-hydroxy vitamin D (25[OH]D), procollagen type I amino-terminal propeptide (P1NP), N-terminal osteocalcin (N-MID), and C-terminal telopeptide of type 1 collagen (β-CTX) were measured using an automated Roche electrochemiluminescence system (Roche Diagnostics GmbH, Germany).
10
Mineral and Bone Metabolism Assessment
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Fasting blood and morning urine samples were collected from all participants. Mineral and bone metabolism markers, including serum calcium, phosphorus, parathyroid hormone (PTH), 25-hydroxyvitamin D (25(OH)D), bone Gla-protein (BGP), and C-terminal telopeptide of type I collagen (β-CTX), were measured using an automated Roche electrochemiluminescence system (Roche Diagnostic Gmbh, Mannheim, Germany) at the central clinical laboratory of Shanghai General Hospital. The urinary levels of calcium were detected, and the values were normalized to creatinine levels (urinary calcium/creatinine ratio; Uca/Ucr) (Siemens Healthcare Diagnostics Inc., Tarrytown, New York, USA) according to the manufacturer's instructions.
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