U0126 (MEK/MAPK inhibitor, 8 μg/10 μl; Promega, Madison, WI, USA), CPZ (TRPV1 inhibitor, 0.4 μg/10 μl; Sigma, St. Louis, MO, USA), DPCPX (A1R inhibitor, 3 μg/10 μl; Tocris Bioscience, Bristol, BS110 QL, UK) were used to inhibit molecular activation in the skin layer. Each solution was injected into the GB34 acupoint 15 min before acupuncture treatment according to the previously described66 (link). Briefly, mice were slightly anesthetized with ether, then each inhibitor was injected into the GB34 acupoint (i.d., 10 µL/each acupoint) using a 0.5 mL syringe (BD Biosciences, San Jose, CA, USA). The control mice were injected with an equal volume of vehicle.
0.5 ml syringe
Manufactured by BD
Sourced in United States
The 0.5 mL syringe is a laboratory equipment used to accurately measure and transfer small volumes of liquids. It has a capacity of 0.5 milliliters and is designed for precise fluid handling in various scientific applications.
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Lab products found in correlation
2 protocols using 0.5 ml syringe
1
Molecular Inhibition at Acupoint GB34
2
Intestinal Lymph Collection and Analysis
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Collection of intestinal lymph from the mesenteric lymphatic duct was performed according to previously described methods (62 , 63 (link)), with some modifications. Briefly, to facilitate visualization of the lymphatic duct, 8 μl of olive oil (Sigma-Aldrich) per gram of body weight was administered by oral gavage to mice 1 hour before general anesthesia using 2% isoflurane. The abdomen was opened by midline incision, and the intestine and colon were retracted to the left side of the animal to identify and locate the mesenteric lymphatic duct. Under a microsurgical microscope (OPMI MD with Universal S3 Stand; Carl Zeiss), a 30-gauge needle linked to 0.5-ml syringe (BD Biosciences) was inserted to the lymphatic duct and advanced for 2 to 3 mm. About 10 to 20 μl of intestinal lymph were obtained from each mouse. Five percent intestinal lymph in complete DMEM was incubated with confluent transformed mouse intestinal epithelial cells (31 (link)) for 16 hours. Cell-cell junctions were analyzed by staining for E-cadherin and ZO-1. Five percent DSS was used as positive control for epithelial cell-cell junction disruption.
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