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Insulin aspart novorapid

Manufactured by Novo Nordisk
Sourced in Denmark, United Kingdom

Insulin aspart (NovoRapid®) is a fast-acting insulin analog developed and manufactured by Novo Nordisk. It is designed to mimic the body's natural insulin response and aid in the management of diabetes.

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2 protocols using insulin aspart novorapid

1

Insulin Absorption Determination Protocol

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Dulbecco’s Modified Eagle’s Medium (DMEM) powder was purchased from Invitrogen (Waltham, MA, USA). Hydrochloric acid (HCl) (1M), sodium hydrogen carbonate (NaHCO3), barium chloride dehydrate (BaCl2, 2H2O), sodium sulfite (Na2SO3), and mucin were bought from Sigma Aldrich. Additionally, fluorescein isothiocyanate (FITC)-labelled insulin stock (Human, recombinant, expressed in yeast, lyophilized powder) was purchased from Sigma Aldrich (St. Louis, MO, USA). HEPES (2-(4-(2-hydroxyethyl)-1-piperazinyl)ethanesulfonic acid) and sodium carbonate (Na2CO3) were from Fisher Scientific (Waltham, MA, USA) and sodium sulfate (Na2SO4) from Merck (Kenilworth, NJ, USA). Insulin aspart (NovoRapid®, Novo Nordisk, Bagsværd, Denmark) was purchased from a local pharmacy. Pepsin was purchased from Promega (Madison, WI, USA).
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2

Biocompatible Polymer for Insulin Delivery

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The material used was a biocompatible Class I photopolymer consisting of methacrylic oligomers and phosphine oxides as photoinitiators branded as Formlabs Dental SG (GoPrint3D, North Yorkshire, UK). The insulin (aspart) (Novorapid) was bought from Novo Nordisk (London, Crawley, UK). The sodium fluorescein was purchased from Sigma Aldrich (Gillingham, Kent, UK). Streptozotocin (≥75% α-anomer basis, ≥98%) and citric J o u r n a l P r e -p r o o f acid were both purchased from Merck Chemical Co. (Darmstadt, Germany). All solvents were of analytical grades.
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