Bovine serum albumin fbs

Human proximal tubular epithelial cells (HK-2) were purchased from ATCC American. Antibodies were obtained from the following sources: polyclonal anti-PKCδ and polyclonal anti-phospho-PKCδ-Tyr-311 from Cell Signaling Technology (Boston, MA) and monoclonal anti-p66Shc and monoclonal anti-phospho-p66Shc-S36 from Abcam (USA). All secondary antibodiesfor Western blot analysis were from ZSGB-BIO (Beijing, China), and secondary antibodies for immunofluorescence were from Jackson ImmunoResearch (West Grove, PA). Rottlerin was obtained from Enzo life sciences (USA), mitochondria isolation kit was purchased from Beyotime (Jiangsu, China), 4′,6-diamidino-2-phenylindole 9 (DAPI) was from Sigma-Aldrich (St. Louis, MO), PKCδ siRNA (h) was from Santa Cruz (USA), Lipofectamine 2000 and MitoSOX were from Invitrogen (USA), 2,7-dichlorodihydrofluorescein diacetate (DCFH-DA) and dihydroethidium (DHE) were from Sigma-Aldrich (USA), and DAB kit was from CWBIO (Beijing, China). Other reagents, including DMEM medium with low glucose (1000 mg/L), bovine serum albumin (FBS), and trypsin, were obtained from GIBCO (USA).

Human kidney proximal tubular epithelial cells (HK-2) were a cell line purchased from the American Type Culture Collection (ATCC, USA). Antibodies were from the following sources: polyclonal anti-TLR4 from Abcam (USA). Monoclonal anti-PGC-1α, monoclonal anti-caspase-3, and NF-κB p65 were from Cell Signaling Technology (Boston, USA). Polyclonal phospho-NF-κB p65 (ser536) antibody was from Bioworld (USA), and polyclonal anti-cytochrome C was from Proteintech (Wuhan, China). Beta-actin and all secondary antibodies for Western blot and immunofluorescence were from Proteintech (Wuhan, China). TAK242 was from MedChem Express (USA), and parthenolide was from Sigma (USA). Plasmids containing pcDNA4 myc PGC-1α (pcDNA4/PGC-1α) was bought from Addgene (USA). Lipofectamine 2000, MitoTracker Red CMXRos, MitoSOX, and TRIzol were purchased from Invitrogen (USA). PrimeScript™ RT reagent Kit with gDNA Eraser and SYBR® Premix Ex Taq™ (Tli RNase H Plus) were from TaKaRa (Japan). Annexin V-FITC Apoptosis Detection Kit was from Beyotime (Shanghai, China). TMRM Detection Kit was from Genmed Scientifics Inc. (USA). Other reagents, including DMEM/F12 medium, bovine serum albumin (FBS), and trypsin, were obtained from Gibco (USA).

Human kidney proximal tubular epithelial cells (HK-2) were a cell line purchased from the American Type Culture Collection (ATCC, USA). Antibodies were from the following sources: polyclonal anti-TLR4 (19811-1-AP), β-actin (20536-1-AP), β-tubulin (10094-1-AP), GSDMD for HK2 (20770-1-AP), and Caspase 1 (22915-1-AP) were from Proteintech (Wuhan, China); Caspase-1 p20 (ab1872), human GSDMD-N (ab210070), and mouse GSDMD-N (ab219800) were from Abcam; NF-κB p65 (#3034; CST) and Phospho-NF-κB p65 (ser536) (#3031; CST) antibody were from Cell Signaling Technology. All secondary antibodies for western blot were from Proteintech (Wuhan, China). TAK-242 was from MedChem Express (HY-11109; USA), and parthenolide was from Sigma (USA). FAM-FLICA Caspase 1 Assay Kit was from ICT (USA). IL-1β ELISA kit was from Huamei (Wuhan, China). Other reagents, including DMEM/F12 medium, bovine serum albumin (FBS) and trypsin were obtained from Gibco (USA).