The EDU assay was carried out using a BeyoClick™ EdU-488 Proliferation Detection Kit (Beyotime, Suzhou, China). In brief, PC cells were cultured in 6-well plates and were allowed to adhere. The primary culture medium was removed and fresh medium was added. Then, 10μM EDU was added into each well and cells were cultured in 37°C for 2.5h. After that, cells were fixed in 4% paraformaldehyde (Beyotime, Suzhou, China) for 15 min and permeabilization using 0.3% Triton X-100 (Boster, Wuhan, China) for 8 min. Then, 500μl Apollo dyeing reaction buffer was added for 40 min in the dark. After staining, the nuclei were stained using DAPI for 10 min. The EDU staining was observed under a fluorescence microscope (Zeiss, Oberkochen, Germany).
Beyoclick edu 488 proliferation detection kit
Manufactured by Beyotime
Sourced in China
The BeyoClick™ EdU-488 Proliferation Detection Kit is a tool designed for detecting and quantifying cell proliferation. It utilizes the incorporation of the thymidine analog EdU (5-ethynyl-2'-deoxyuridine) into newly synthesized DNA, which is then detected by a fluorescent dye.
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5 protocols using beyoclick edu 488 proliferation detection kit
1
Quantifying Cell Proliferation with EDU Assay
2
Cell Proliferation Assay Using EDU
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The EDU assay was carried out using a BeyoClick™ EdU-488 Proliferation Detection Kit (Beyotime, Suzhou, China). In brief, while PC cells adherence in 6-well plate, primary medium was removed and fresh medium were added. Then, total 10μM EDU was injected into each well and cells were cultured in 37°C for 2.5h. Following by xation using 4% paraformaldehyde (Beyotime, Suzhou, China) for 15 min and permeabilization using 0.3% Triton X-100 (Boster, Wuhan, China) for 8 min, total 500μl Apollo dyeing reaction buffer was then added for 40 min in dark environment. After staining the nuclear using DAPI for 10 min, the proportion of EDU was detected using uorescence microscopy.
3
Cell Proliferation Assay Using EDU
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EDU assay was carried out using a BeyoClick™ EdU-488 Proliferation Detection Kit (Beyotime, Suzhou, China). In brief, while PC cells adherence in 6-well plate, primary medium was removed and fresh medium were added. Then, total 10μM EDU was injected into each well and cells were cultured in 37°C for 2.5h. Following by xation using 4% paraformaldehyde (Beyotime, Suzhou, China) for 15 min and permeabilization using 0.3% Triton X-100 (Boster, Wuhan, China) for 8 min, total 500μl Apollo dyeing reaction buffer was then added for 40 min in dark environment. After staining the nuclear using DAPI for 10 min, the proportion of EDU was detected using uorescence microscopy.
4
Cell Proliferation Assay Using EDU
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EDU assay was carried out using a BeyoClick™ EdU-488 Proliferation Detection Kit (Beyotime, Suzhou, China). In brief, while PC cells adherence in 6-well plate, primary medium was removed and fresh medium were added. Then, total 10μM EDU was injected into each well and cells were cultured in 37°C for 2.5h. Following by xation using 4% paraformaldehyde (Beyotime, Suzhou, China) for 15 min and permeabilization using 0.3% Triton X-100 (Boster, Wuhan, China) for 8 min, total 500μl Apollo dyeing reaction buffer was then added for 40 min in dark environment. After staining the nuclear using DAPI for 10 min, the proportion of EDU was detected using uorescence microscopy.
5
Cell Proliferation Assay Using EDU
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The EDU assay was carried out using a BeyoClick™ EdU-488 Proliferation Detection Kit (Beyotime, Suzhou, China). In brief, while PC cells adherence in 6-well plate, primary medium was removed and fresh medium were added. Then, total 10μM EDU was injected into each well and cells were cultured in 37°C for 2.5h. Following by xation using 4% paraformaldehyde (Beyotime, Suzhou, China) for 15 min and permeabilization using 0.3% Triton X-100 (Boster, Wuhan, China) for 8 min, total 500μl Apollo dyeing reaction buffer was then added for 40 min in dark environment. After staining the nuclear using DAPI for 10 min, the proportion of EDU was detected using uorescence microscopy.
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