Indirect immunofluorescence staining was performed as described previously (33 (link)–34 (link), 37 (link)). Background fluorescence was determined by applying the secondary conjugated antibody alone and by replacement of the primary antibody with non-immune serum. Slides were successively stained with β-catenin antibody (clone 14; 1:100; BD Biosciences, San Jose, CA), followed by incubation with secondary antibodies labeled with Alexa Fluor 488 Dye (Molecular Probes Life Technologies). Images were collected using a confocal laser scanning system (LSM 510, Karl Zeiss, Gottingen, Germany).
β catenin antibody clone 14
Manufactured by BD
The β-catenin antibody (clone 14) is a laboratory reagent used for the detection and analysis of β-catenin, a key mediator of the Wnt signaling pathway. It is a monoclonal antibody that recognizes the β-catenin protein.
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2 protocols using β catenin antibody clone 14
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Indirect Immunofluorescence Staining Protocol
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Indirect Immunofluorescence Staining Protocol
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Indirect immunofluorescence staining was performed as described previously 35 (link), 36 (link), 39 (link). Background fluorescence was determined by applying the secondary conjugated antibody alone and by replacement of the primary antibody with nonimmune serum. Slides were successively stained with β-catenin antibody (clone 14; 1:100; BD Biosciences), followed by incubation with secondary antibodies labeled with Alexa Fluor 488 Dye (Molecular Probes Life Technologies). Images were collected using a confocal laser scanning system (LSM 510; Karl Zeiss).
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