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Mouse anti rabbit igg conformation specific

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Mouse anti-rabbit IgG (conformation specific) is a secondary antibody used to detect the presence of rabbit primary antibodies in immunoassays. It is designed to recognize the native conformation of rabbit immunoglobulin G (IgG) molecules.

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Lab products found in correlation

Giantin, by Abcam (2 mentions) Phospho s6 ser240 244, by Cell Signaling Technology (2 mentions) Lamp2, by Santa Cruz Biotechnology (2 mentions) Phospho p70 s6 kinase thr389, by Cell Signaling Technology (2 mentions) Slc38a9, by Merck Group (2 mentions) Phospho ulk1 ser757, by Cell Signaling Technology (2 mentions) Lamtor1, by Cell Signaling Technology (2 mentions) Lamtor3, by Cell Signaling Technology (2 mentions) Atp6v1b2, by Abcam (2 mentions) Atp6v1a, by GeneTex (2 mentions) Lbpa z plbpa echelon, by Tebu-Bio (2 mentions) Flag tag (flag), by Merck Group (2 mentions) Raptor, by Cell Signaling Technology (2 mentions) Mms 101p, by Fortrea (2 mentions) Hrp conjugated antibodies, by Jackson ImmunoResearch (2 mentions) Lamp1, by BD (2 mentions) Goat anti rabbit igg, by Abcam (1 mentions) Ki67 antibody, by Abcam (1 mentions) Phospho ikkα β ser176 180, by Cell Signaling Technology (1 mentions) Tnfr1, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

IgG rabbit (9 citations) Rabbit anti-IgG (8 citations) Mouse Anti-rabbit IgG Conformation Specific (L27A9; (5 citations) Conformation-specific anti-rabbit IgG (3 citations) IgG (Mouse) (3 citations)

5 protocols using mouse anti rabbit igg conformation specific

1

Antibody Profiling of Nutrient Sensing Pathways

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Antibodies used were SLC38A9 (HPA043785 Sigma), LAMTOR1 (8975 Cell Signaling), LAMTOR3 (8169 Cell Signaling), RAGA (4357 Cell Signaling), RAGC (5466 Cell Signaling), phospho-p70 S6 Kinase (Thr389) (9234 Cell Signaling), phospho-S6 (Ser240/244) (2215 Cell Signaling), phospho-ULK1 (Ser757) (6888 Cell Signaling), raptor (2280 Cell Signaling), ATP6V1B2 (ab73404 Abcam), ATP6V1A (GTX110815 GeneTex), mouse anti-rabbit IgG (conformation specific) (3678 Cell Signaling), LAMP1 (555798 Pharmingen and ab25630 Abcam), LAMP2 (sc-18822 Santa Cruz), CD63 (H5C6 DSHB), LBPA (Z-PLBPA Echelon, Tebu-bio), EEA1 (sc33585 Santa Cruz), Giantin (ab24586 Abcam), p70 S6 kinase (sc-230 Santa Cruz), ULK1 (8054 Cell Signaling), Tubulin (ab7291 Abcam), RCC1 (sc55559 Santa Cruz), HA (H6533 Sigma, 3724 Cell Signaling, MMS-101P Covance or sc-805 Santa Cruz), V5 (ab9116 Abcam), His (A7058 Sigma), FLAG (F7425 Sigma). The secondary antibodies used were goat anti-mouse AlexaFluor568 (A-11004 and A-11031 Molecular probes), goat anti-rabbit AlexaFluor568 (A-11036 Molecular probes), goat anti-mouse AlexaFluor488 (A-11001 Molecular probes), goat anti-rabbit AlexaFluor488 (A-11008 Molecular probes) and HRP-conjugated antibodies (Jackson ImmunoResearch).
Rebsamen M., Pochini L., Stasyk T., de Araújo M.E., Galluccio M., Kandasamy R.K., Snijder B., Fauster A., Rudashevskaya E.L., Bruckner M., Scorzoni S., Filipek P.A., Huber K.V., Bigenzahn J., Heinz L.X., Kraft C., Bennett K.L., Indiveri C., Huber L.A, & Superti-Furga G. (2015). SLC38A9 is a component of the lysosomal amino acid-sensing machinery that controls mTORC1. Nature, 519(7544), 477-481.
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2

Immunoprecipitation and Western Blot of Wheat TaS6K1

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Wheat embryos were ground in liquid nitrogen and then resuspended in lysis buffer A. After centrifugation at 13,000 × g for 10 min, 4 μg of the anti-TaS6K1 polyclonal antibody was added to the cleared supernatant and incubated with rotation for 6 h. Then, 20 μl of a 50% slurry of protein G-Sepharose was added, and the incubation was continued for 4 h. Captured immunoprecipitates were washed three times with lysis buffer. Samples were resolved by SDS-PAGE, and proteins were transferred to a PVDF membrane and visualized by immunoblotting with either the anti-TaS6K1 polyclonal antibody or phospho-AtS6K antibody (p-T449; Abcam, Cambridge, United Kingdom). A goat anti-rabbit IgG (Abcam, Cambridge, United Kingdom) or mouse anti-rabbit IgG (Conformation Specific; Cell Signaling Technology, Danvers, MA, United States) antibody, respectively, was used to detect the primary antibodies.
Smailov B., Alybayev S., Smekenov I., Mursalimov A., Saparbaev M., Sarbassov D, & Bissenbaev A. (2020). Wheat Germination Is Dependent on Plant Target of Rapamycin Signaling. Frontiers in Cell and Developmental Biology, 8, 606685.
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3

Immunoprecipitation and Western Blot Analysis

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Intervertebral disc tissue samples were treated with ice-cold IP lysis buffer on ice for 30 min, centrifuged at 12,000 g for 30 min. The supernatant of the lysate was collected. About 1 μg protein antibody and 50 μL protein A-agarose beads were added to the appropriate amount of lysate and incubated at 4°C overnight. Afterwards, the agarose beads were centrifuged to the bottom of the tube at 4°C, 3,000 rpm for 3 min. The supernatant was extracted out and the agarose beads were washed 3–4 times with 1 ml lysis buffer. Lysis buffers were carefully collected and added 2 × SDS loading buffer and boiled for 10 min. Finally, western blot was used for analysis.
Mouse Anti-rabbit IgG (Conformation Specific) (1:5,000, No. 5127, Cell Signaling Technology, Beverly, MA, United States) was used as second antibody.
Dai F., Yu P., Yu Z., Jiang H., Ma Z, & Liu J. (2021). Yiqi Huoxue Recipe Delayed Intervertebral Disc Degeneration by Activating Autophagy. Frontiers in Pharmacology, 12, 705747.
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4

Antibody Profiling of Nutrient Sensing Pathways

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Antibodies used were SLC38A9 (HPA043785 Sigma), LAMTOR1 (8975 Cell Signaling), LAMTOR3 (8169 Cell Signaling), RAGA (4357 Cell Signaling), RAGC (5466 Cell Signaling), phospho-p70 S6 Kinase (Thr389) (9234 Cell Signaling), phospho-S6 (Ser240/244) (2215 Cell Signaling), phospho-ULK1 (Ser757) (6888 Cell Signaling), raptor (2280 Cell Signaling), ATP6V1B2 (ab73404 Abcam), ATP6V1A (GTX110815 GeneTex), mouse anti-rabbit IgG (conformation specific) (3678 Cell Signaling), LAMP1 (555798 Pharmingen and ab25630 Abcam), LAMP2 (sc-18822 Santa Cruz), CD63 (H5C6 DSHB), LBPA (Z-PLBPA Echelon, Tebu-bio), EEA1 (sc33585 Santa Cruz), Giantin (ab24586 Abcam), p70 S6 kinase (sc-230 Santa Cruz), ULK1 (8054 Cell Signaling), Tubulin (ab7291 Abcam), RCC1 (sc55559 Santa Cruz), HA (H6533 Sigma, 3724 Cell Signaling, MMS-101P Covance or sc-805 Santa Cruz), V5 (ab9116 Abcam), His (A7058 Sigma), FLAG (F7425 Sigma). The secondary antibodies used were goat anti-mouse AlexaFluor568 (A-11004 and A-11031 Molecular probes), goat anti-rabbit AlexaFluor568 (A-11036 Molecular probes), goat anti-mouse AlexaFluor488 (A-11001 Molecular probes), goat anti-rabbit AlexaFluor488 (A-11008 Molecular probes) and HRP-conjugated antibodies (Jackson ImmunoResearch).
Rebsamen M., Pochini L., Stasyk T., de Araújo M.E., Galluccio M., Kandasamy R.K., Snijder B., Fauster A., Rudashevskaya E.L., Bruckner M., Scorzoni S., Filipek P.A., Huber K.V., Bigenzahn J., Heinz L.X., Kraft C., Bennett K.L., Indiveri C., Huber L.A, & Superti-Furga G. (2015). SLC38A9 is a component of the lysosomal amino acid-sensing machinery that controls mTORC1. Nature, 519(7544), 477-481.
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5

Comprehensive Immunoblotting Protocol

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Cleaved caspase 3, JNK1, phospho JNK1 (Thr183/Tyr185), IKKα, phospho IKKα/β (Ser176/180), IKKε, phospho IKKε (Ser172), IκB, NFκB, PKC phospho substrate, Akt, phospho Akt (Ser473), PKCα, PKCζ, phospho PKC pan (βII Ser660), TNFR1, TNFR2, Fas, DR3, DR5, TRAF2, TRADD, FADD and mouse anti-rabbit IgG (conformation specific) antibodies were obtained from Cell Signaling Technology. PTPRN2 (HPA026656, SAB4502542 and HPA006900), FLAG(M2), HA, β-actin, MEMO1, PARP1 monoclonal (C-2-10), and α-tubulin monoclonal antibodies were obtained from Sigma. Ki-67 antibody was obtained from Abcam. c-Myc antibody was obtained from Santa Cruz Biotechnology. μ-calpain antibody was obtained from EMD Millipore. Sintaxin 6, Calnexin and E cadherin antibodies were obtained from BD Biosciences. Phospho TRAF2 (Ser11) antibody was kindly provided by Dr. Hasem Habelhah at the University of Iowa (USA).
Sorokin A.V., Nair B.C., Wei Y., Aziz K.E., Evdokimova V., Hung M.C, & Chen J. (2015). Aberrant expression of proPTPRN2 in cancer cells confers resistance to apoptosis. Cancer research, 75(9), 1846-1858.
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