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Ck04 20

Manufactured by Dojindo Laboratories
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CK04-20 is a laboratory equipment product manufactured by Dojindo Laboratories. It serves as a cell counting device, providing a reliable method for determining the number of cells in a sample.

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Lab products found in correlation

Infinite m200 pro, by Tecan (1 mentions) Cck 8 kit, by Dojindo Laboratories (1 mentions) Sirna reagent, by Horizon Discovery (1 mentions) Human ifn γ quantikine elisa kit, by R&D Systems (1 mentions)

4 protocols using ck04 20

1

Cell Proliferation Assay with U0126

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Cells were plated on laminin-coated 96-well plates at a density of 1000–3000 cells/well. At designated time points, CCK8 reagent (Dojindo, CK04-20) was added into each well according to manufacturer’s instructions. After incubation for 3–5 hours, OD450 absorbance of each well was screened by a microplate reader (Tecan, infinite M200PRO). For cell response to U0126, cells were plated out for 24 hours before U0126 was added into the wells.
Yang R., Chen L.H., Hansen L.J., Carpenter A.B., Moure C.J., Liu H., Pirozzi C.J., Diplas B.H., Waitkus M.S., Greer P.K., Zhu H., McLendon R.E., Bigner D.D., He Y, & Yan H. (2017). Cic loss promotes gliomagenesis via aberrant neural stem cell proliferation and differentiation. Cancer research, 77(22), 6097-6108.
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2

Cell Growth Assay Using CCK-8

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Stably or transiently transfected cells were used for cell growth assays. The proliferation assay was performed in accordance with the manufacturer’s instructions (CCK8-Kit; CK04-20, Dojindo, Rockville, MD).
Kang M.H., Choi H., Oshima M., Cheong J.H., Kim S., Lee J.H., Park Y.S., Choi H.S., Kweon M.N., Pack C.G., Lee J.S., Mills G.B., Myung S.J, & Park Y.Y. (2018). Estrogen-related receptor gamma functions as a tumor suppressor in gastric cancer. Nature Communications, 9, 1920.
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3

siRNA-mediated proliferation assay in H23 cells

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Dharmacon siRNA reagent (#G-005805-01) was delivered into H23 cell through Reverse transfection method67 (link). Briefly, siRNA and lipid (Lipofectamine® 2000 Reagent, 11668019) were mixed and added to 96-well plates. Then, H23 cells were added to the plates (10,000 cells per well). Cell proliferation rate was measured on day 3 by CCK8 assay (CK04-20, Dojindo Molecular Technologies, Inc.). During screening, siRNAs targeting EGFP (5′- CGTGATCTTCACCGACAAGAT-3′) are included in the upper-most and lower-most rows of each plate, which served as negative control. The screening was conducted once.
Gao L., Hu Y., Tian Y., Fan Z., Wang K., Li H., Zhou Q., Zeng G., Hu X., Yu L., Zhou S., Tong X., Huang H., Chen H., Liu Q., Liu W., Zhang G., Zeng M., Zhou G., He Q., Ji H, & Chen L. (2019). Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition. Nature Communications, 10, 1665.
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4

Anti-CD3 Antibody-Mediated T Cell Activation

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LEAF purified anti-human CD3 antibody (clone UCHT1, BioLegend, 300438) was immobilized (5 μg/ml) on an assay plate overnight at 4°C. After removal of unbound anti-human CD3 antibody, uncharacterized PBMC (C.T.L., CTL-UP1, lot no. HHU20180509) effector cells (3 × 104 cells per well), indicated doses of purified antibodies, and HCC1954 target cells (1 × 104 cells per well) were added and incubated for 72 hours at 37°C in a humidified incubator with 5% CO2. Supernatants were collected and assayed for IFN-γ secretion using a human IFN-γ Quantikine ELISA kit (R&D Systems, SIF50). Cell viability was analyzed by measuring absorbance at 450 nm after adding cell counting kit-8 (Dojindo, CK04-20) according to the manufacturer’s protocol.
You G., Lee Y., Kang Y.W., Park H.W., Park K., Kim H., Kim Y.M., Kim S., Kim J.H., Moon D., Chung H., Son W., Jung U.J., Park E., Lee S., Son Y.G., Eom J., Won J., Park Y., Jung J, & Lee S.W. (2021). B7-H3×4-1BB bispecific antibody augments antitumor immunity by enhancing terminally differentiated CD8+ tumor-infiltrating lymphocytes. Science Advances, 7(3), eaax3160.
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