Male C57BL/6 mice were obtained from Daehan Biolink (DBL, Seoul, Korea). The mice had ad libitum access to water and food in the laboratory animal facility at PNU. Eight-week-old mice were randomly divided into 2 groups for lysophosphatidylinositol analysis (Figure 6 ). Control C57BL/6 mice (n = 5) were fed with a normal chow diet for 4 weeks while high-fat diet C57BL/6 mice (n = 5) were fed a synthetic diet supplemented with 60% w/w) fat (HFD, Efeed, Korea) for 4 weeks (Figure 6 ). The other sets were randomly divided into 5 groups for fatty liver induction and CID16020046 treatment (Figure 7 ): Control C57BL/6 mice fed a normal chow diet for 4 weeks (n = 5), high-fat diet C57BL/6 mice fed a synthetic diet supplemented with 60% (w/w) fat (HFD, Efeed, Korea) for 4 weeks (n = 5), high-fat diet C57BL/6 mice treated with CID16020046 treatment for the last week (n = 5), C57BL/6 mice treated with O-1602 (1 mg/kg, i.p. injection three times per week) for 4 weeks (n = 5), and C57BL/6 mice treated with O-1602 for 4 weeks plus CID16020046 (1 mg/kg, i.p. injection, five consecutive days for the last week) (n = 5) (Figure 7 ). The animal protocol used in this study was reviewed and approved by the PNU Institutional Animal Care Committee with respect to the ethics of the procedures and animal care (PNU-20192335).
Male c57bl 6 mice
Manufactured by Daehan Biolink
Sourced in United States
Male C57BL/6 mice are a widely used inbred strain of laboratory mice. They are a common model organism for various research applications.
Automatically generated - may contain errors
Lab products found in correlation
Stereotaxic frame, by Kopf Instruments (2 mentions)
Hamilton syringe 30 s needle, by KD Scientific (2 mentions)
Syringe pump, by KD Scientific (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (2 mentions)
Standard irradiated chow, by Purina (2 mentions)
Male icr mice, by Daehan Biolink (1 mentions)
Chloral hydrate, by Merck Group (1 mentions)
Rompun, by Bayer (1 mentions)
Ketamine, by Bayer (1 mentions)
High fat diet (hfd), by Research Diets (1 mentions)
Male sprague dawley rats, by Daehan Biolink (1 mentions)
34 protocols using male c57bl 6 mice
1
Dietary Modulation of Fatty Liver in Mice
2
Animal Models for Hair Growth and Inflammation
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Male C57BL/6 mice for hair growth study and male ICR mice for inflammation study were purchased from the Daehan Biolink (Eumseong, Korea), and housed in a room with constant environmental conditions (23±2℃; 45-65% relative humidity; 12-hour light-dark cycle; 150-300 lux brightness). Pellet feed and purified water were available ad libitum. All the animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) of Chungbuk National University (CBNU), Korea, and conducted according to the Standard Operation Procedures (SOP) of the Laboratory Animal Research Center, CBNU.
3
LPS-induced Sepsis Model in C57BL/6 Mice
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Male C57BL/6 mice (6 weeks old) were obtained from Daehan Biolink Co. (Daejeon, Republic of Korea). All animals were housed in accordance with the guidelines for the care and use of laboratory animals. The guidelines were adopted and promulgated by Sangji University according to the requirements demonstrated by the National Institutes of Health. All the experimental protocols were approved based on the Institutional Animal Care and Use Committee (IACUC) of Sangji University before the beginning of the study (IACUC Animal approval protocol No.2020-10). The mice were housed in a cage and fed standard laboratory chow in the animal room with 12 h dark/light cycles and constant condition (20 ± 5°C temperature, 40–60% humidity) for a week. The mice randomly were assigned to one of five groups (n = 6 per group). The C57BL/6 mice were intraperitoneal injected with PBS or LPS (30 mg/kg dissolved in PBS). MF (50 and 100 mg/kg) was injected intraperitoneally 1 h before LPS injection. Survival was monitored for 108 h after LPS administration. Four hours after LPS injection, peripheral blood and liver samples were obtained from each mouse.
4
Mice Acclimation and Experimental Protocols
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Male C57BL/6 mice (age: 6–7 weeks old; weight: 20–25 g) were used in this study (Daehan Biolink, Eum-seong, Korea). Mice were acclimated for at least 1 week before the experiments and maintained with a 12-h light/dark cycle with free access to water and food. The animals were randomly divided, and five mice were placed in each cage (room temperature 25°C and humidity of 55%) in a clean room. Their weight was monitored every 2 days (Supplementary Figure S1 ). This study was carried out in accordance with recommendations in the guidelines for the Care and Use of Laboratory Animals of the National Institutes of Health. All experimental protocols used in this study were approved by the Institutional Animal Care and Use Committee (IACUC) at Daejeon University (approval no. DJUARB2016-40).
5
Topical Application of Nelumbinis Semen Extract Enhances Hair Growth
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Male C57BL/6 mice (4-week-old) purchased from Dae-Han Biolink Co. (Eumsung, Chungbuk, Korea) were allowed to acclimate for 3 weeks. The mice were housed in cages at 23 ± 1°C and 55 ± 5% humidity, with a 12 h light and dark cycle and free access to food and water. All experimental protocols followed the guidelines established for the management and handling of laboratory animals and were approved by the Institutional Animal Ethics Committee of Chung-Ang University, Korea (registration no. 201900112). Twenty-one mice (n = 7/group) were randomized into the DMSO (NC), Nelumbinis Semen (NS) extract, and 3% minoxidil (PC) treatment groups. The backs of the mice were shaved with animal clippers at 7 weeks of age. Afterward, 200 μl DMSO, 200 μl of NS extract (1,000 ppm), or 3% minoxidil was administered daily on the dorsal skin for 3 weeks using a brush, as described in previous studies [23 (link), 24 (link), 26 (link)]. At 21 days, the mice were anesthetized via intraperitoneal injection of 1.2% avertin to obtain dorsal skin samples.
6
Kainic Acid-Induced Hippocampal Injury in Mice
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Male C57BL/6 mice (8 weeks old, 22-23 g) were obtained from Daehan Biolink (Eumseong, Korea). Mice were housed in a controlled environment and fully provided with food and water. All surgical experiments were performed in accordance with approved animal protocols and guidelines established by the Animal Care Committee of Kyungpook National University (number KNU 2012-37). Anesthetized C57BL/6 mice using chloral hydrate (360 mg/kg, Sigma, St. Louis, MO) were positioned in a stereotaxic frame (David Kopf Instruments, Tujunga, CA). As previously described with modifications [18 (link)], each mouse received a unilateral injection of KA (0.2 μg in 4 μL PBS; Sigma) using a 10 μL Hamilton syringe (30 S needle) attached to a syringe pump (KD Scientific, New Hope, PA) into the hippocampus (AP: −2.0 mm; ML: −1.2 mm; DV: −1.5 mm, relative to the bregma). Mice treated with 4 μL PBS (Sigma), which showed no effect on neurotoxicity in the hippocampus, were used as controls. After the injection, the needle was left in place for an additional 5 min before being slowly retracted.
7
Silibinin Mitigates Kainic Acid-Induced Toxicity
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Male C57BL/6 mice (8-week-old, 20~25 g) were obtained from Daehan Biolink (Eumseong, Korea). The mice were housed in a controlled environment and provided with ad libitum food and water. Mice were divided into six groups as follows: i) Group 1: PBS-treated group (control group); ii) Group 2: 200 mg/kg silibinin-treated group; iii) Group 3: KA-treated group; iv) Group 4: KA+50 mg/kg silibinin-treated group; v) Group 5: KA+100 mg/kg silibinin-treated group; vi) Group 6: KA+200 mg/kg silibinin-treated group. All animal experiments were performed in accordance with approved animal protocols and guidelines established by the Animal Care Committee of Kyungpook National University (No. KNU 2016-42).
8
Animal Housing and Handling Protocol
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Male C57BL/6 mice (body weights 25-27 g) and male SD rats (200-220 g) were procured from Daehan Biolink (Eumseong, Korea), and housed in a room with constant environmental conditions (23±2℃; 55±10% relative humidity; 12-hour light-dark cycle; 150-300 lux brightness). Pellet feed and purified water were available ad libitum. All the animal experiments were conducted according to the Standard Operation Procedures (SOP), and approved by the Institutional Animal Care and Use Committee of Chungbuk National University, Korea. For biosafety, the investigators were fully protected with sterilized clothes, masks, and gloves on SOP.
9
Mouse Model for Pain Research
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Male C57BL/6 mice, aged six weeks postnatal were provided by the Daehan Biolink (Emsung, Korea). The animals were housed in a controlled vivarium on an artificial 12-h light-dark cycle (light cycle: 7 AM to 7 PM) with ad libitum access to chow and water (n = 5/cage). All experimental protocols were approved by the Kyung Hee University Animal Care and Use Committee (KHUASP (SE) 20-147; approved April 2020) and conducted in accordance with the ethical guidelines of the International Association for the Study of Pain [19 (link)].
10
Acute MPTP-Induced Parkinson's Model
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Male C57BL/6 mice (6 weeks old, 20–23 g) were obtained from Daehan Biolink Co. Ltd. (Chungbuk, Korea). The animals were maintained under temperature- and light-controlled conditions (20–23 °C under a 12 h light cycle) and were provided food and water ad libitum. The animals were randomly allocated to three groups of 10 to 12 animals: to a vehicle control group, an MPTP control group, or an MPTP + 1 μmol/kg IAGIP group. All animals were acclimatized for 1 week before drug administration. MPTP was injected i.p. at 20 mg/kg four times at 2 h intervals to induce acute PD symptoms, and IAGIP (1 μmol/kg dissolved in PBS) was injected i.v. four times at 2, 6, 24, and 48 h after the last MPTP injection. A schematic of the in vivo experiments is provided in Figure 4 A. The animal protocol used in this study was reviewed and approved beforehand by the Institutional Animal Care Committee of Pusan National University (PNU-IACUC; Approval Number PNU-2021-2903).
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