Alexa fluor 488 or 568 goat anti rabbit

Manufactured by Thermo Fisher Scientific

Alexa Fluor 488 or 568 goat anti-rabbit is a fluorescently labeled secondary antibody used for detection and visualization in various immunoassays and imaging techniques. It is produced in goats and specifically recognizes and binds to rabbit primary antibodies. The Alexa Fluor 488 and 568 dyes provide bright, photostable fluorescent signals that can be detected using appropriate filter sets.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 568 donkey anti mouse, by Thermo Fisher Scientific (1 mentions) Rpte cells, by Lonza (1 mentions) Regm bulletkit, by Lonza (1 mentions) Lysotracker red dnd 99, by Thermo Fisher Scientific (1 mentions) Low melting temperature agarose, by Lonza (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) A11122, by Merck Group (1 mentions) To pro 3, by Thermo Fisher Scientific (1 mentions) Anti insulin, by Abcam (1 mentions) Anti smooth muscle actin, by Merck Group (1 mentions) Anti cytokeratin, by Abcam (1 mentions) Anti smooth muscle actin, by Abcam (1 mentions) Anti glucagon, by Agilent Technologies (1 mentions) Anti gfp, by Thermo Fisher Scientific (1 mentions)

3 protocols using alexa fluor 488 or 568 goat anti rabbit

Generating and Characterizing BK Polyomavirus in Renal Proximal Tubular Epithelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

RPTE cells were obtained from Lonza and used at passage 6 and 7 for all experiments. RPTE cells were grown in renal epithelial basal media supplemented with the REGM bulletkit (Lonza).
BK-Dunlop inserted into a pGEM vector (kindly provided by M. Imperiale) was digested using BamHI, then purified and re-ligated. The re-ligated genome was transfected into a T75 flask of RPTE cells in 5% FCS REGM. After three weeks, the cells were split into three T150 flasks and left for a further three weeks before harvesting. The cells were freeze thawed three times to release the virus and assayed using a fluorescent focus assay (protocols based on [57 (link)]).
The primary antibodies used were PAb597 against SV40 VP1 (kindly provided by W. Atwood), P5G6 against BKPyV VP1 (kindly provided by D. Galloway), ab53977 against SV40 VP1 (Abcam), ab53983 against SV40 VP2 and VP3 (Abcam), PAb416 against SV40 T-antigen (Abcam), H4A3 against LAMP-1 (Developmental Studies Hybridoma Bank) and ab6160 against tubulin [YL1/2] (Abcam). Secondary antibodies used were Alexa Fluor 568 donkey anti-mouse and Alexa Fluor 488 or 568 goat anti-rabbit (Invitrogen). LysoTracker red DND-99 was obtained from Life Technologies.

Evans G.L., Caller L.G., Foster V, & Crump C.M. (2015). Anion homeostasis is important for non-lytic release of BK polyomavirus from infected cells. Open Biology, 5(8), 150041.

+ Open protocol

+ Expand

In situ Hybridization and Immunostaining of Embryos

Check if the same lab product or an alternative is used in the 5 most similar protocols

Embryos were fixed in 4% paraformaldehyde (PFA) in PBS. In situ hybridization was carried out as described [62 (link)]. Images were acquired and morphometric measurements were carried out manually with Fiji software.
Immunostaining was performed using a standard protocol. The following antibodies were used: anti-phospho-Histone H3 antibody (1:3,000, rabbit, Upstate, 06–570), and Alexa Fluor 488 or 568 goat anti-rabbit (1:500, Invitrogen). Embryos were counterstained with 4',6-diamidino-2-phenylindole (DAPI, 0.1 μg/mL, Invitrogen), mounted in 0.75% low melting temperature agarose (Lonza) in 0.3% Danieau’s solution (LMTD agarose), and imaged with the Quorum spinning disk confocal microscope (SDCM) using a 10x objective lens (10x). A Z-stack of over 200 μm was acquired at a step size of 3 μm and projected in Fiji. The number of nuclei was quantified using Analyze Particles plugin in Fiji.

Liu Y., Sepich D.S, & Solnica-Krezel L. (2017). Stat3/Cdc25a-dependent cell proliferation promotes embryonic axis extension during zebrafish gastrulation. PLoS Genetics, 13(2), e1006564.

+ Open protocol

+ Expand

Immunostaining of Chick/Quail Grafts

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated grafts were fixed in 4% formaldehyde in PBS. Cryosections were cut at 5µm thickness. Immunohistochemical analysis of sectioned chick or quail tissue was as published (25 (link)). The following primary antibodies were applied overnight at 4°C: Anti-GFP (Invitrogen A11122, 1:200), Anti-smooth muscle actin (Sigma A2547 1:200), Anti-smooth muscle actin (Abcam Ab5694 1:200), Anti-sarcomeric myosin (MF20), QCPN (DSHB undiluted), 8F3 (DSHB 1:25), Anti-cytokeratin (Abcam Ab9377, 1:100), QH1 (DSHB, 1:200), Anti-insulin (Abcam Ab63820, 1:100) and Anti-glucagon (DAKO, A0565, 1:200). The following secondary antibodies were applied at a 1:500 dilution for 90 min at room temperature: Alexa fluor 488 or 568 Goat anti-rabbit (Invitrogen); Alexa fluor 488 or 568 Goat anti-mouse (Invitrogen). TOPRO-3 (Invitrogen T3605) at 1 µmol/L was applied with secondary antibody. Sections were imaged in Z-stacks using a LSM510 META Confocal with 0.4 µm optical slices. All IHC images presented in figures are Z-projections. The mesothelial layer was distinguished by morphology combined with cytokeratin staining. Nuclei within the mesothelial layer were manually identified and then subsequently identified as QCPN positive or negative.

Winters N.I., Williams A.M, & Bader D.M. (2014). Resident progenitors, not exogenous migratory cells, generate the majority of visceral mesothelium in organogenesis. Developmental biology, 391(2), 125-132.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!