Allprep isolation kit

Manufactured by Qiagen

Sourced in Germany

The AllPrep Isolation Kit is a product designed for the simultaneous purification of DNA, RNA, and proteins from a single biological sample. It utilizes a unique technology to enable efficient extraction and isolation of these biomolecules.

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Lab products found in correlation

Allprep dna rna ffpe kit, by Qiagen (2 mentions) Beadarray reader, by Illumina (2 mentions) Agilent 2100 bioanalyzer, by Agilent Technologies (2 mentions) Nanodrop 1000 spectrophotometer, by Thermo Fisher Scientific (2 mentions) Human 660w quad beadchip array, by Illumina (1 mentions) Prism 7900ht, by Thermo Fisher Scientific (1 mentions) Magnetic activated cell sorting (macs), by Miltenyi Biotec (1 mentions) Wizard genomic dna purification kit, by Promega (1 mentions) Sh800 cell sorter, by Sony (1 mentions) Totalprep rna amplification kit, by Illumina (1 mentions) Sentrix human 6v3 whole genome expression beadchips sentrix human wg 6, by Illumina (1 mentions) Beadstudio software, by Illumina (1 mentions) Infinium humanmethylation450 beadchip assay, by Illumina (1 mentions) Epitect bisulfite kit, by Qiagen (1 mentions) Qiamp dna mini kit, by Qiagen (1 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Macs column, by Miltenyi Biotec (1 mentions) Allprep dna rna mini kit, by Qiagen (1 mentions) Lymphoprep, by STEMCELL (1 mentions) Allprep dna rna protein mini kit, by Qiagen (1 mentions)

Close spelling variants of this product

AllPrep kit (307 citations) AllPrep DNA/RNA isolation kit (20 citations) AllPrep DNA/RNA FFPE isolation kit (7 citations) AllPrep DNA/RNA/miRNA isolation kit (6 citations) AllPrep DNA/RNA/Protein isolation kit (4 citations) Allprep RNA isolation kit (3 citations) AllPrep DNA and RNA isolation kit (2 citations) AllPrep DNA/RNA/miRNA Universal isolation kit (2 citations)

12 protocols using allprep isolation kit

Genetic Variant Identification in Pancreatic Diseases

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For each sample DNA was extracted from whole blood or from paraffin-embedded pancreatic tissues of patients and controls using the AllPrep Isolation Kit (Qiagen, Hilden, Germany) or the Qiagen-mini kit (Qiagen, Hilden, Germany), according to the manufacturer's protocol. Genotyping was performed using TaqMan (ABI, Applied Biosystems, Foster City, CA, USA) and KASPar (KBioscence, Hoddesdon, UK) technologies. The order of DNA samples was randomized on plates in order to ensure that similar numbers of cases and controls were analyzed in each batch. Detection was performed using an ABI PRISM 7900 HT or Viia7 sequence detection system with SDS 2.2 or Viia7 software (Applied Biosystems, Foster City, CA, USA). Genotyping for British and Dutch controls was performed in the context of a genome-wide association study using the Human 660W-Quad BeadChip array according to manufacturer's instructions (Illumina, San Diego, CA, USA). For quality control, duplicates of 10% of the samples were interspersed throughout the plates. In addition, we discarded all the samples that had a call rate < 75%.

Campa D., Pastore M., Gentiluomo M., Talar-Wojnarowska R., Kupcinskas J., Malecka-Panas E., Neoptolemos J.P., Niesen W., Vodicka P., Fave G.D., Bueno-de-Mesquita H.B., Gazouli M., Pacetti P., Di Leo M., Ito H., Klüter H., Soucek P., Corbo V., Yamao K., Hosono S., Kaaks R., Vashist Y., Gioffreda D., Strobel O., Shimizu Y., Dijk F., Andriulli A., Ivanauskas A., Bugert P., Tavano F., Vodickova L., Zambon C.F., Lovecek M., Landi S., Key T.J., Boggi U., Pezzilli R., Jamroziak K., Mohelnikova-Duchonova B., Mambrini A., Bambi F., Busch O., Pazienza V., Valente R., Theodoropoulos G.E., Hackert T., Capurso G., Cavestro G.M., Pasquali C., Basso D., Sperti C., Matsuo K., Büchler M., Khaw K.T., Izbicki J., Costello E., Katzke V., Michalski C., Stepien A., Rizzato C, & Canzian F. (2016). Functional single nucleotide polymorphisms within the cyclin-dependent kinase inhibitor 2A/2B region affect pancreatic cancer risk. Oncotarget, 7(35), 57011-57020.

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Quantifying HIV RNA and DNA

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RNA and DNA were extracted (Allprep isolation kit, Qiagen) from total blood CD4⁺ T-cells (MACS; Miltenyi; purity >95%). Cell-associated unspliced (CA-US) RNA (lower limit of detection (LLOD)=1 copy/well), and total HIV DNA was quantified as previously described^{[11 (link)]}. Plasma HIV RNA was quantified using both a standard Roche (LLOD=20 copies/mL) and an ultra-sensitive single copy assay (SCA; LLOD=0.13 copies/mL), as previously described^{[11 (link), 12 (link)]}.

EVANS V.A., VAN DER SLUIS R.M., SOLOMON A., DANTANARAYANA A., McNEIL C., GARSIA R., PALMER S., FROMENTIN R., CHOMONT N., SÉKALY R.P., CAMERON P.U, & LEWIN S.R. (2018). PD-1 contributes to the establishment and maintenance of HIV-1 latency. AIDS (London, England), 32(11), 1491-1497.

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FFPE DNA Extraction and Characterization

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DNA was isolated from formalin fixed, paraffin-embedded (FFPE) samples following the manufacturer’s protocol for AllPrep DNA/RNA FFPE kit (Qiagen, Hilden, Germany) as previously described [23 (link), 24 (link)]. Tumor and adjacent normal regions were identified on H&E stained slides and aligned to tissue paraffin blocks. An 18-gauge sterile needle was used to core 2–4 samples from each region. Deparaffinization was performed using the xylene/ethanol method with the only modification being that samples were digested using proteinase K at 56 °C for 20–24 min. DNA isolation was then completed using the Allprep Isolation kit (Qiagen, Hilden, DE) following manufacturer protocol. Each sample was analyzed using a Nanodrop spectrophotometer for purity (260:230 and 260:280 ratios) and concentration was determined using 1uL of sample with the Qubit 2.0 Fluorometer and measured with a bioanalyzer as described [25 (link)]. DNA extraction for MDA8788–6 cell line was performed using Wizard® Genomic DNA Purification Kit (Promega, Madison, WI).

Heft Neal M.E., Birkeland A.C., Bhangale A.D., Zhai J., Kulkarni A., Foltin S.K., Jewell B.M., Ludwig M.L., Pinatti L., Jiang H., McHugh J.B., Marentette L., McKean E.L, & Brenner J.C. (2021). Genetic analysis of sinonasal undifferentiated carcinoma discovers recurrent SWI/SNF alterations and a novel PGAP3-SRPK1 fusion gene. BMC Cancer, 21, 636.

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Sorting Liver CD8+ Lymphocytes by CD69

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Liver lymphocytes from 6 control patients were sorted using fluorescent label-conjugated mouse monoclonal antibodies for CD3, CD8 and CD69 (Supplementary Materials). Liver CD3 + CD8+ lymphocytes were sorted into a CD69+ and CD69- fraction on a Sony SH800 cell sorter (Sony biotechnology Inc., CA, USA). After sorting, RNA was extracted with the AllPrep isolation kit (Qiagen, CA, USA).

Stelma F., de Niet A., Sinnige M.J., van Dort K.A., van Gisbergen K.P., Verheij J., van Leeuwen E.M., Kootstra N.A, & Reesink H.W. (2017). Human intrahepatic CD69 + CD8+ T cells have a tissue resident memory T cell phenotype with reduced cytolytic capacity. Scientific Reports, 7, 6172.

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Transcriptome Analysis of Frozen Tissue

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For RNA isolation, the frozen tissue slices were submerged in liquid nitrogen and gently ground with a polypropylene micropestle (Eppendorf, Hamburg, Germany) in a 2 ml Eppendorf tube. Total RNA was isolated with the AllPrep Isolation kit (Qiagen), following the manufacturer's protocol. RNA integrity was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto). Only samples with an RNA integrity number of at least seven were used for further analyses. The total RNA prepared from individual samples was analysed on Sentrix Human‐6v3 Whole Genome Expression BeadChips (Sentrix Human WG‐6; Illumina). To synthesize first and second strand cDNA and for amplifying biotinylated cRNA, the Illumina Totalprep RNA Amplification kit was used. Hybridisation to the BeadChip was performed according to the manufacturer's instructions. Subsequently, the arrays were scanned with a BeadArray Reader (Illumina).

Bauer A.S., Nazarov P.V., Giese N.A., Beghelli S., Heller A., Greenhalf W., Costello E., Muller A., Bier M., Strobel O., Hackert T., Vallar L., Scarpa A., Büchler M.W., Neoptolemos J.P., Kreis S, & Hoheisel J.D. (2017). Transcriptional variations in the wider peritumoral tissue environment of pancreatic cancer. International Journal of Cancer, 142(5), 1010-1021.

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Genome-wide DNA Methylation Analysis

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DNA was isolated with the AllPrep Isolation kit (Qiagen, Hilden, Germany), following the manufacturer's protocol. We performed on 12 normal and 12 PDAC samples the Infinium Human Methylation 450 BeadChip assay of Illumina (San Diego), which interrogates 485,000 methylation sites across the human genome, using 1 µg of DNA per sample. Bisulfite‐converted DNA (EpiTect Bisulfite kit; Qiagen) acted as template for whole‐genome amplification, enzymatic digestion, followed by a DNA clean‐up process and hybridisation to the BeadChip. The samples were washed and scanned with the BeadArray Reader (Illumina). From the signal intensities, the degree of DNA methylation was analysed using the Illumina Bead studio software.

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ABO Genotyping for Blood Typing

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The ABO blood group was inferred through genotypes with DNA extraction (QIAmp DNA Mini Kit or AllPrep isolation kit, Qiagen). Genotyping was conducted using a polymerase chain reaction–based genotyping system (KASPar SNP, KBiosciences). Genotypes calls were done using real-time polymerase chain reaction (ViiA 7 Real-Time PCR System, ThermoFisher Applied Biosystems). For blood group computation, we used rs505922 to distinguish between O and non-O, and rs8176746 from ABO A and B alleles. Using the genotyping combination of these 2 single-nucleotide variants, the blood group was reconstructed.^{18 (link)}

Capurso G., Crippa S., Vanella G., Traini M., Zerboni G., Zaccari P., Belfiori G., Gentiluomo M., Pessarelli T., Petrone M.C., Campa D., Falconi M, & Arcidiacono P.G. (2020). Factors Associated With the Risk of Progression of Low-Risk Branch-Duct Intraductal Papillary Mucinous Neoplasms. JAMA Network Open, 3(11), e2022933.

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PBMC Isolation and RNA/DNA Extraction

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Blood samples from BCN02 participants were processed using Lymphoprep (STEMCELL technologies) and 2 × 10⁶ dry pelleted PBMCs were frozen until use. DNA and RNA were isolated simultaneously from the same sample (AllPrep DNA/RNA Mini Kit, Qiagen) and were frozen until use. PBMC's were isolated from blood samples from REDUC trial participants using Ficoll separation followed by CD4 T-cells isolation using a CD4 T-cell isolation kit and magnetic-activated cell sorting (MACS) columns (Miltenyi Biotec, purity >95%). Isolated CD4 cells were lysed using AllPrep lysis buffer and lysates were stored at -80°C until RNA was extracted (AllPrep isolation kit, Qiagen).

Oriol-Tordera B., Esteve-Codina A., Berdasco M., Rosás-Umbert M., Gonçalves E., Duran-Castells C., Català-Moll F., Llano A., Cedeño S., Puertas M.C., Tolstrup M., Søgaard O.S., Clotet B., Martínez-Picado J., Hanke T., Combadiere B., Paredes R., Hartigan-O'Connor D., Esteller M., Meulbroek M., Calle M.L., Sanchez-Pla A., Moltó J., Mothe B., Brander C, & Ruiz-Riol M. (2022). Epigenetic landscape in the kick-and-kill therapeutic vaccine BCN02 clinical trial is associated with antiretroviral treatment interruption (ATI) outcome. EBioMedicine, 78, 103956.

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Simultaneous DNA and RNA Extraction

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DNA and RNA from colon and ileum biopsies (stored in RNAlater solution by Thermo Fisher) were isolated using the AllPrep DNA/RNA/Protein mini kit (Qiagen, catalog #80004). Briefly, the biopsies were homogenized in RLT Lysis buffer (Allprep isolation kit, Qiagen, catalog #80204) using stainless-steel beads (5 mm, Qiagen, Catalog #69989) on a Qiagen TissueLyser II. Total RNA and DNA were simultaneously purified from the tissue lysates following the manufacturer's protocol. On-column DNAse digestion was performed during the RNA extraction. Quantification of DNA and RNA was performed using a Nanodrop (ND-1000) spectrophotometer.

Singh S., Giron L.B., Shaikh M.W., Shankaran S., Engen P.A., Bogin Z.R., Bambi S.A., Goldman A.R., Azevedo J.L., Orgaz L., de Pedro N., González P., Giera M., Verhoeven A., Sánchez-López E., Pandrea I., Kannan T., Tanes C.E., Bittinger K., Landay A.L., Corley M.J., Keshavarzian A, & Abdel-Mohsen M. (2024). Distinct intestinal microbial signatures linked to accelerated systemic and intestinal biological aging. Microbiome, 12, 31.

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Nucleic Acid Extraction from Tissues

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Genomic DNA and total RNA were isolated from fresh‐frozen tissue samples with the AllPrep Isolation Kit (Qiagen, Hilden, Germany), following the protocol suggested by the manufacturer. RNA integrity was evaluated using an Agilent 2100 Bioanalyzer (Agilent Technologies, Palo Alto, CA, USA). DNA quantities and qualities were determined using a NanoDrop 1000 spectrophotometer (ThermoFisher Scientific, Waltham, MA, USA).

Manoochehri M., Wu Y., Giese N.A., Strobel O., Kutschmann S., Haller F., Hoheisel J.D., Moskalev E.A., Hackert T, & Bauer A.S. (2020). SST gene hypermethylation acts as a pan‐cancer marker for pancreatic ductal adenocarcinoma and multiple other tumors: toward its use for blood‐based diagnosis. Molecular Oncology, 14(6), 1252-1267.

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