C57BL/6NCR (strain code 556) (CD45.2+) and B6-Ly5.1/Cr (stain code 564) (CD45.1+) were purchased from Charles River Laboratories. Rosa26mTmG, Pf4-cre, Kitlfl/fl, Lepr-cre, and KitlGFP/+ mice were from The Jackson Laboratories. Cxcr4fl/fl, Lyz2-cre, Il7ra-cre, CD4-cre, and Rosa26tdtomato/+ mice were from internal colonies. Flk2-cre mice were a gift from Dr. E. Camilla Forsberg (University of California, Santa Cruz). Although Flk2-cre transgene is inserted into Y-chromosome, our previous work showed the hematopoietic cell composition in bone marrow and secondary lymphoid organs of Flk2-cre transgenic mice is indistinguishable from their littermate controls. All mice analyzed were 8-20 week old, were maintained under specific pathogen-free conditions at Yale Animal Resources Center.
Kitlfl fl
Manufactured by Jackson ImmunoResearch
Kitlfl/fl is a laboratory reagent used in scientific research. It is a genetic construct that contains the Kitl gene flanked by loxP sites, which allow for conditional manipulation of the gene expression in cells or tissues of interest. The core function of Kitlfl/fl is to facilitate the study of the Kitl gene and its role in various biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Pf4 cre, by Jackson ImmunoResearch (1 mentions)
C57bl 6ncr, by Charles River Laboratories (1 mentions)
Rosa26mtmg, by Jackson ImmunoResearch (1 mentions)
Lepr cre, by Jackson ImmunoResearch (1 mentions)
B6 ly5.1 cr, by Charles River Laboratories (1 mentions)
D12492, by Research Diets (1 mentions)
Cl 316 243, by R&D Systems (1 mentions)
Adipoq cre, by Jackson ImmunoResearch (1 mentions)
2 protocols using kitlfl fl
1
Murine Hematopoietic Cell Lineages
2
Adipose-Specific Regulation of KitL
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All mice used in this study were purchased from the Jackson Laboratory, including Kitlfl/fl (stock n. 017861), Adipoq-Cre (stock n. 010803), Osx1-Cre (stock n. 006361), and KitlEGFP (stock n. 017860). All animals were kept on a 14 hour (h):10 h light: dark cycle in the animal facility at the University of Minnesota, Minneapolis, MN, USA. Mice were group-housed, with free access to water and either a standard chow diet or 60% high fat diet (Research Diets, D12492). 1 mg/kg BW of CL-316, 243 (R&D Systems, #1499, diluted in saline) were intraperitoneally (i.p.) injected when indicated. For thermoneutral housing, mice born at 22°C were transferred to a room maintained continuously at 30°C at the age indicated. All procedures involving animals were conducted within Institutional Animal Care and Use Committee guidelines under approved protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!