From the cervix, cervical exfoliated cell specimens were collected from each participant using a Cyto-Brush (QIAGEN, Gaithersburg, MD, USA) for HPV DNA assays. According to the age of the participant and the stage of cervical cancer, the samples were divided into five groups (<25, 25–34, 35–44, 45–54, and ≥55).
Cytobrush
Manufactured by Qiagen
Sourced in United States
The Cytobrush is a lab equipment product designed for collecting cell samples. It is a sterile, single-use brush that is used to obtain cellular material from various body sites for analysis.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using cytobrush
1
Cervical Exfoliated Cell Collection
2
Cervical Exfoliated Cells Screening Protocol
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Another cervical exfoliated cells sample was collected by cyto-brush (QIAGEN, Valencia, CA) among women with HPV infection. ThinPrep 2000 (Hologic Inc.) and SurePath liquid-based Pap test (BD, USA) were used for the TCT test which was performed by Kingmed Center for Clinical Laboratory Corporation (Guangzhou, China). Cytological slides were read by three cytopathologist. Cervical cell samples were categorized according to the Bethesda System (2001)
[18 (link),19 (link)] as follows: normal; atypical squamous cells of unknown significance (ASCUS); low-grade squamous intraepithelial neoplasia (LSIL); and high-grade squamous intraepithelial lesion (HSIL) or worse (i.e., cases of squamous cell carcinoma were combined with HSIL cases for the purposes of analysis).
[18 (link),19 (link)] as follows: normal; atypical squamous cells of unknown significance (ASCUS); low-grade squamous intraepithelial neoplasia (LSIL); and high-grade squamous intraepithelial lesion (HSIL) or worse (i.e., cases of squamous cell carcinoma were combined with HSIL cases for the purposes of analysis).
3
Oral Mouthwash and Cytobrush Sampling Protocol
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Self‐collected oral mouthwash samples were collected using the NHANES methodology (Gillison et al., 2012 (link)). A 50‐ml sterile collection cup filled with 10 ml of Scope (original mint flavor) was provided to each participant. They were asked to rinse/gargle with the mouthwash for 30 seconds, spit the mouthwash into the collection cup while trying not to spill the liquid, and close the cup tightly. Afterward, participants used a cytobrush (QIAGEN) to self‐collect an oral sample (Read et al., 2012 (link)). They gently brushed the gums' inner and outer areas for at least 10 strokes. Samples were obtained using the digeneHc2 DNA collection device from QIAGEN. Both samples were performed during the visit and supervised by the study coordinators.
4
Cervical Specimen Collection and Cytology Evaluation
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The present study was approved by the Ethics Committee of the School of Medicine, Jinan University (Guangzhou, China). Cervical specimens were collected with a Cytobrush (Qiagen, Inc., Valencia, CA, USA) and placed into a ThinPrep Pap test vial (Hologic, Inc., Marlborough, MA, USA) containing 20 ml PreservCyt Solution (Hologic, Inc.). Referral Pap specimens were used for the ThinPrep cytologic test, which was performed by Kingmed Center for Clinical Laboratory Corporation (Guangzhou, China) using the ThinPrep 2000 System (Hologic, Inc.) and evaluated for routine screening cytology. A Pap smear was positive for a squamous intraepithelial lesion (SIL) if there was a low (LSIL) or high (HSIL) grade SIL, as classified according to the Bethesda Classification System (16 (link)). Excluding cases with other types of uterine disease and without a history of hysterectomy, a total of 164 cases [120 LSIL (73.2%) and 44 HSIL (26.8%)] were recruited. A total of 296 control samples without an intraepithelial lesion or malignancy were recruited from the area of residence of the cases. The study was explained to each individual and written consent for their participation was obtained.
5
Cervical Cell Collection and Screening
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Cervical exfoliated cells were collected by cytobrush (Qiagen China Co., Ltd, Shanghai, China) from ectocervix and endocervix of the uterus of every woman by cervical scrapings. These were then fixed in TCT cytological solution for 15 min. ThinPrep 2000 (Hologic Inc., USA) and a SurePath liquid-based Pap test (BD, USA) were used for the TCT test. ThinPrep smears were screened by two independent cytopathologists. The smears were stained using standard Pap methodology and classified using the The Bethesda System (TBS; 2001) criteria [19 (link),20 (link)] as follows: negative for intraepithelial lesion or malignancy (NILM); atypical squamous cells of unknown significance (ASCUS); atypical squamous cells-high grade (ASC-H); low-grade squamous intraepithelial neoplasia (LSIL); and high-grade squamous intraepithelial lesion (HSIL) or worse.
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