Human d dimer elisa kit

The concentrations of serum D-dimer, C-reactive protein, ferritin, cardiac troponin I and interleukin 6 were determined with a human D-dimer ELISA kit, a human CRP ELISA kit, a human ferritin ELISA kit, a human cardiac troponin I ELISA kit and an IL-6 ELISA kit (Abcam, Cambridge, UK) measured on a PHOmo microplate reader (Autobio Diagnostics, Zhengzhou, China) according to the manufacturer's instructions. For hospitalized patients, day 1, day 7, day 14 and day 28 samples were used, while for ambulatory patients, day 1, day 14 and day 28 were used. The detection limits for D-dimer, C-reactive protein, ferritin, cardiac troponin I and interleukin 6 were 2.36 ng/mL, 5.36 pg/mL, 5 ng/mL, 4.4 pg/mL and 3 pg/mL respectively.

We assessed plasma samples by using commercially available kits. For plasminogen activator inhibitor 1 (PAI-1) we used Human Serpin E1/PAI-1 Quantikine ELISA Kit (R&D Systems, https://www.rndsystems.com) or Human PAI-1 Platinum Kit (eBioscience, https://www.thermofisher.com). We used Human t-Plasminogen Activator/tPA Quantikine ELISA Kit (R&D Systems) to measure tissue plasminogen activator (tPA). We used Human Thrombomodulin/BDCA-3 Quantikine ELISA Kit (R&D Systems) or Human Thrombomodulin ELISA Kit (Innovative Research Inc., https://www.innov-research.com) to measure thrombomodulin (THBD) and Human Thrombin-Antithrombin Complex ELISA Kit (Abcam, https://www.abcam.com) to measure thrombinantithrombin (TAT) complexes. To assess endothelial protein C receptor (EPCR) we used Human EPCR DuoSet ELISA Kit (R&D Systems), for D-dimer we used Human D-Dimer ELISA Kit (Abcam), for a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS-13) we used Human ADAMTS13 Quantikine ELISA Kit (R&D Systems), for P-selectin we used Human CD62P ELISA Kit (Abcam), for hepatocyte growth factor (HGF) we used Human HGF Quantikine ELISA Kit (R&D Systems), for von Willebrand factor (vWF) we used vWF Human ELISA Kit (ThermoFisher, https://www.thermofisher.com), and for tissue factor we used Human Coagulation Factor III/Tissue Factor Quantikine ELISA Kit (R&D Systems).