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Nm 003068 trcn0000284362

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NM_003068/TRCN0000284362 is a laboratory equipment product by Merck Group. It is a tool used for scientific research and analysis purposes. No further details can be provided in an unbiased and factual manner without extrapolation.

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Lab products found in correlation

Anti slug antibody, by Cell Signaling Technology (2 mentions)

2 protocols using nm 003068 trcn0000284362

1

Stable Knockdown of SLUG in Lung Cancer Cells

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Lentiviral transduction particles carrying short hairpin RNA (shRNA) sequence against human SNAI2 and control non-target sequence (Sigma-Aldrich; NM_003068/TRCN0000284362 and TRCN0000015389 and SHC002V) were used to knockdown SLUG expression in H460 and A549 cells, according to the manufacturer’s protocol. Briefly, cells were incubated with lentiviral particles in the presence of hexadimethrine bromide (8 µg/mL) for 36 hours. Infected cells were allowed to recover for 48 hours and were cultured for 28 days with puromycin-containing medium (1 µg/mL). The stable knockdown cells were identified by Western blotting using anti-SLUG antibody (Cell Signaling Technology, Beverly, MA; 9585) and were cultured in puromycin-free RPMI 1640 medium.
Luanpitpong S., Li J., Manke A., Brundage K., Ellis E., McLaughlin S.L., Angsutararux P., Chanthra N., Voronkova M., Chen Y.C., Wang L., Chanvorachote P., Pei M., Issaragrisil S, & Rojanasakul Y. (2015). SLUG is required for SOX9 stabilization and functions to promote cancer stem cells and metastasis in human lung carcinoma. Oncogene, 35(22), 2824-2833.
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2

Stable Knockdown of SLUG in Lung Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lentiviral transduction particles carrying short hairpin RNA (shRNA) sequence against human SNAI2 and control non-target sequence (Sigma-Aldrich; NM_003068/TRCN0000284362 and TRCN0000015389 and SHC002V) were used to knockdown SLUG expression in H460 and A549 cells, according to the manufacturer’s protocol. Briefly, cells were incubated with lentiviral particles in the presence of hexadimethrine bromide (8 µg/mL) for 36 hours. Infected cells were allowed to recover for 48 hours and were cultured for 28 days with puromycin-containing medium (1 µg/mL). The stable knockdown cells were identified by Western blotting using anti-SLUG antibody (Cell Signaling Technology, Beverly, MA; 9585) and were cultured in puromycin-free RPMI 1640 medium.
Luanpitpong S., Li J., Manke A., Brundage K., Ellis E., McLaughlin S.L., Angsutararux P., Chanthra N., Voronkova M., Chen Y.C., Wang L., Chanvorachote P., Pei M., Issaragrisil S, & Rojanasakul Y. (2015). SLUG is required for SOX9 stabilization and functions to promote cancer stem cells and metastasis in human lung carcinoma. Oncogene, 35(22), 2824-2833.
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