Cd14 fitc staining
The CD14 FITC staining is a laboratory equipment product used for the detection and analysis of CD14 proteins on the surface of cells. It utilizes fluorescein isothiocyanate (FITC) as the fluorescent label to visualize and quantify the expression of CD14, a marker commonly associated with monocytes and macrophages. This product allows researchers to study the presence and distribution of CD14-positive cells in various biological samples.
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2 protocols using cd14 fitc staining
Quantification of NIS and PENDRIN Proteins
Isolation and Characterization of Human Monocytes
Peripheral blood monocytes were obtained from leucocyte residues from healthy donors provided by the Blood and Tissue Bank (Barcelona, Spain). Succinctly, peripheral blood mononuclear cells were depleted of CD3+ cells using the RosetteSepTM Human CD3 Depletion Cocktail (StemCell Technologies, Seattle, WA) during a Ficoll-Paque density centrifugation (GE Healthcare, Uppsala, Sweden) and monocytes were then positively selected by MagniSort™ Human CD14 Positive Selection Kit (eBioscience, San Diego, CA), as recommended by the manufacturer. Monocytes were assessed for purity by CD14-FITC staining (BD, San Jose, CA), obtaining >95% CD14+ cells analysed in a Canto II flow cytometer (BD).
Culture medium comprised RPMI 1640 medium supplemented with 100 µg/ml streptomycin, 100 IU/ml penicillin and 5% heat-inactivated human AB serum (H4522, Sigma, St Louis, MO). Cells were counted using PerfectCount Microspheres (Cytognos, Salamanca, Spain) and viability was >94% as determined by 7-AAD exclusion by flow cytometry (FACSCanto II, BD).
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